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Comparative Evaluation of Phytochemicals and Antisickling Activities of Extracts of Fruits of Phoenix Dactylifera and Leaves of Terminalia Catappa
Subject area: Science,Engineering and Technology · Area of research: Science/Chemistry/medicine
Abstract
Dried and blended fruits of Phoenix dactylifera L. and leaves of Terminalia catappa L. were soaked with methanol, the water soluble extracts were obtained by decoction while the methanol soluble extracts was macerated and fractionated with n-hexane, chloroform and ethyl-acetate. The chemical screening of extracts, revealed the presence of alkaloids, anthraquinones, cardiac glycosides, flavonoids, saponins and tannins. The plants extracts were evaluated against sickled red blood cells using p-hydroxybenzoic acid and normal saline as positive and negative controls. The method involves inhibition of sodium metabisulphite induced sickling of HbSS red blood cells, collected from sickle cell patients. The methanol soluble extracts PDME and TCME (5mg/ml) showed antsickling activities of 78.2% and 82.8% at 180min of incubation respectively while the chloroform soluble extracts PDCE and TCCE (5mg/ml) demonstrated antisickling activities of 77.6% and 86.7% respectively at 180min incubation time. The n-hexane soluble extracts, PDHE and TCHE (5mg/ml) showed antisickling activities of 71.8% and 78.3% while that of the ethyl-acetate soluble extracts, PDEE and TCEE(5mg/ml) indicated antisickling activities of 70.2% and 83.5% respectively at 180min incubation time compared to p-hydroxybenzoic acid (5mg/ml) with 76.2% inhibition at 180min time of incubation and the water soluble extracts, PDWE and TCWE (5mg/ml) which showed 10.9% and 12.4% sickled red blood cells at 180min incubation time indicating antisickling activities of 89.1 and 87.6 respectively. Compared to the untreated SS suspension with 83.6% sickled red blood cells at 180mm incubation. These results showed that the fruits of date palm and leaves of Terminalia catappa are good agents for sickle cell disease therapy.
Keywords
phytochemicals, sickle cell anaemia, Antisickling activities, phoenix dactylifera, terminalia catappa.
How to cite this paper
@article{1702877,
author = {Ahmed Momoh Hassan},
title = {Comparative Evaluation of Phytochemicals and Antisickling Activities of Extracts of Fruits of Phoenix Dactylifera and Leaves of Terminalia Catappa},
journal = {Iconic Research And Engineering Journals},
year = {2021},
volume = {5},
number = {1},
pages = {345-360},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1702877.pdf},
abstract = {Dried and blended fruits of Phoenix dactylifera L. and leaves of Terminalia catappa L. were soaked with methanol, the water soluble extracts were obtained by decoction while the methanol soluble extracts was macerated and fractionated with n-hexane, chloroform and ethyl-acetate. The chemical screening of extracts, revealed the presence of alkaloids, anthraquinones, cardiac glycosides, flavonoids, saponins and tannins. The plants extracts were evaluated against sickled red blood cells using p-hydroxybenzoic acid and normal saline as positive and negative controls. The method involves inhibition of sodium metabisulphite induced sickling of HbSS red blood cells, collected from sickle cell patients. The methanol soluble extracts PDME and TCME (5mg/ml) showed antsickling activities of 78.2% and 82.8% at 180min of incubation respectively while the chloroform soluble extracts PDCE and TCCE (5mg/ml) demonstrated antisickling activities of 77.6% and 86.7% respectively at 180min incubation time. The n-hexane soluble extracts, PDHE and TCHE (5mg/ml) showed antisickling activities of 71.8% and 78.3% while that of the ethyl-acetate soluble extracts, PDEE and TCEE(5mg/ml) indicated antisickling activities of 70.2% and 83.5% respectively at 180min incubation time compared to p-hydroxybenzoic acid (5mg/ml) with 76.2% inhibition at 180min time of incubation and the water soluble extracts, PDWE and TCWE (5mg/ml) which showed 10.9% and 12.4% sickled red blood cells at 180min incubation time indicating antisickling activities of 89.1 and 87.6 respectively. Compared to the untreated SS suspension with 83.6% sickled red blood cells at 180mm incubation. These results showed that the fruits of date palm and leaves of Terminalia catappa are good agents for sickle cell disease therapy.},
keywords = {phytochemicals, sickle cell anaemia, Antisickling activities, phoenix dactylifera, terminalia catappa.},
month = {July},
}