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Analytical Method Development and Validation for Simultaneous Estimation of 5-methyl-N-{[2-(morpholin-4-yl)quinolin-3-yl]methyl}-1,3-thiazol-2-amine in Bulk Form
Subject area: Science,Engineering and Technology · Area of research: Analytical Chemistry
Abstract
A reverse phase high performance liquid chromatographic technique for validating 5-methyl-N-[2-(morpholin-4-yl)quinolin-3-yl]methyl-1,3-thiazol-2-amine (MMQMTA) in its original form has been developed. A HYPERSIL (250 x 4.6 mm, 5 m) ID column was used for the chromatography, and the mobile phase was a 60:40 mixture of methanol and ammonium acetate with a flow rate of 1.0 ml/min. The detection wavelength was 235 nm. MMQMTA had a retention time of 6.812 min. The approach yields linearity responses at concentrations of 4?24 mg/ml of MMQMTA. MMQMTA has a detection limit of 0.021 g/ml and an area of 2.61. Area 6.812 and 0.05 g/ml were reported as the MMQMTA limits of quantification, and the MMQMTA recovery rate was determined to be 99.98%. The technique is helpful for ensuring and monitoring the quality of pharmaceutical and bulk formulations.
Keywords
5-methyl-N-{[2-(morpholin-4-yl)quinolin-3-yl]methyl}-1,3-thiazol-2-amine, MMQMTA, RP-HPLC, validation.
How to cite this paper
@article{1703742,
author = {Yogita Shinde, Kalpana Patankar-Jain},
title = {Analytical Method Development and Validation for Simultaneous Estimation of 5-methyl-N-{[2-(morpholin-4-yl)quinolin-3-yl]methyl}-1,3-thiazol-2-amine in Bulk Form},
journal = {Iconic Research And Engineering Journals},
year = {2022},
volume = {6},
number = {3},
pages = {106-111},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1703742.pdf},
abstract = {A reverse phase high performance liquid chromatographic technique for validating 5-methyl-N-[2-(morpholin-4-yl)quinolin-3-yl]methyl-1,3-thiazol-2-amine (MMQMTA) in its original form has been developed. A HYPERSIL (250 x 4.6 mm, 5 m) ID column was used for the chromatography, and the mobile phase was a 60:40 mixture of methanol and ammonium acetate with a flow rate of 1.0 ml/min. The detection wavelength was 235 nm. MMQMTA had a retention time of 6.812 min. The approach yields linearity responses at concentrations of 4?24 mg/ml of MMQMTA. MMQMTA has a detection limit of 0.021 g/ml and an area of 2.61. Area 6.812 and 0.05 g/ml were reported as the MMQMTA limits of quantification, and the MMQMTA recovery rate was determined to be 99.98%. The technique is helpful for ensuring and monitoring the quality of pharmaceutical and bulk formulations.},
keywords = {5-methyl-N-{[2-(morpholin-4-yl)quinolin-3-yl]methyl}-1,3-thiazol-2-amine, MMQMTA, RP-HPLC, validation.},
month = {September},
}