Malaria is endemic and a life-threatening disease been caused by the bite of a female anopheles mosquito which destroys the red blood cells. Nano carrier drug delivery system is of great interest in malaria research for improving the quality of health care delivery. This research work focused on development of inorganic silica nanoparticles as efficient delivery system for antimalarial drugs of Arthemeter (ATM) and Lumefantrine (LFT). The mesoporous silica nanoparticles (MSNPs) both amino modified mobile crystalline matter (aMCM-41) and mobile crystalline matter (MCM-41) were synthesized by co-condensation and sol-gel methods respectively. ATM and LFT antimalarial drugs were loaded in both MCM-41 and aMCM-41 with chloroform as the solvent under varying effects of time (1hr, 3hrs and 6hrs), pH (Neutral and Acidic) and temperature (25oC and 40oC) respectively. The synthesized nano carrier (MCM-41 and aMCM-41) and nanodrugs fit well for their expected properties as depicted from Fourier-Transform infrared spectroscopy (FT-IR), Nitrogen Physiosorption Isotherm, UV-Visible Spectroscopy, in vitro kinetic study and in vivo measurement using P. berghei NK65. The drug loading capacities (DLC) and Entrapment Efficiency (EE) of the nano carriers were determined using UV-Visible spectrophotometry. The FT-IR depicts major functional groups of the silanol group (Si-OH) and silaxone (Si-O) which absorbed at 3450 cm-1 and 964 cm-1 respectively for MCM-41, while after amino functionalization the silanol group was obstructed. The nanodrugs show only the functional groups of MSNPs due to the drugs encapsulation. The synthesized MSNPs (MCM-41 and aMCM-41) have average pore diameter of 5.1617 nm and 2.9778 nm respectively as expected for the mesoporous materials which decreases due to adsorption of ATM encapsulated in MCM-41 and ATM encapsulated in aMCM-41 to 4.395 nm and 2.5551 nm accordingly. ATM encapsulates in MCM-41 and aMCM-41: MCM-41? ATM and aMCM-41? ATM have the highest DLC of 79% and 81% and EE of 65% and 67% respectively when compared with LFT with DLC of 77% and 75% and EE of 50% and 54% respectively attributed to the size effect. The in-vitro kinetic studies of the drugs and their nanodrugs showed that MSNPs loaded ATM has the highest percentage of drugs released compared with LFT. The in-vivo measurement of the combination of ATM and LFT loaded MCM-41 and aMCM-41 shows better bio performance for plasmodia clearance in infected mice on the third day compared to other nanodrugs and the parent drugs.Therefore; this shows the satisfactory of the synthesized nano carrier for the delivery of the antimalarial drugs.
Antimalarial Drugs (Arthemeter and Lumefantrine), Delivery System, Mesoporous Silica Nanoparticles (MSNPs), Co-condensation and Sol-gel, Encapsulate.
Oladipo O. P , S. A Amolegbe , Adewuyi , Akinremi; O.G Adomowo , Balogun; Obaleye "Synthesis and Therapeutic Performance of Mesoporous Silica Nanocarrier for Antimalarial Drug Delivery System" Iconic Research And Engineering Journals Volume 6 Issue 5 2022 Page 76-82
Oladipo O. P , S. A Amolegbe , Adewuyi , Akinremi; O.G Adomowo , Balogun; Obaleye "Synthesis and Therapeutic Performance of Mesoporous Silica Nanocarrier for Antimalarial Drug Delivery System" Iconic Research And Engineering Journals, 6(5)