A Review on Column Chromatographic Techniques as Separation Method

Dr. Tushar P. Dukre; Sagar S. Sangale; Akash K. Shelke; Nilesh M. Chattar; Dhananjay D. Narsale

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A Review on Column Chromatographic Techniques as Separation Method

Dr. Tushar P. Dukre Sagar S. Sangale Akash K. Shelke Nilesh M. Chattar Dhananjay D. Narsale

Subject area: Biological & Medical Sciences · Area of research: Pharmacognosy

Abstract

A technique frequently used to extract compounds from complicated mixtures is column chromatography. Column chromatography, as opposed to planar chromatography, packs the stationary phase or polymer (resins) into a column. The packed stationary phase then is passed through the mobile phase toachieve separation. This division may serve analytical or preparatory purposes. Chromatography, which relies on the interaction of a stationary and a mobile phase, is a crucial method for purifying mixture components.

Keywords

Column chromatography, Mobile phase, Stationary phase, Types of column chromatography, etc.

References

[1] OzlemCoskun, Separation techniques: Chromatography, North Clin Istanbul 2016;3(2):156–60.

[2] http://www.rnlkwc.ac.in/pdf/study-material/physiology/column%20chromatography.pdf

[3] Das M, Dasgupta D. Pseudo-affinity column chromatography based rapid, purification procedure for T7 RNA polymerase. Prep BiochemBiotechnol. 1998; 28: 339-348.

[4] https://microbenotes.com/column-chromatography/

[5] https://www.biomadam.com/types-of-column-chromatography/

[6] Burden DW, Whitney DB. Protein Purification by Column Chromatography. Biotechnology Proteins to PCR. 1995; 93-124.

[7] Lodha Luxminarayan1, Sharma Neha1, Viswas Amit2, A Review on Chromatography Techniques, Asian Journal of Pharmaceutical Research and Development, Vol. 5 (2) March–April. 2017:1-08

How to cite this paper

Dr. Tushar P. Dukre, Sagar S. Sangale, Akash K. Shelke, Nilesh M. Chattar, Dhananjay D. Narsale "A Review on Column Chromatographic Techniques as Separation Method" Iconic Research And Engineering Journals Volume 6 Issue 7 2023 Page 116-120

Dr. Tushar P. Dukre, Sagar S. Sangale, Akash K. Shelke, Nilesh M. Chattar, Dhananjay D. Narsale "A Review on Column Chromatographic Techniques as Separation Method" Iconic Research And Engineering Journals, vol. 6, no. 7, Jan. 2023

Dr. Tushar P. Dukre, Sagar S. Sangale, Akash K. Shelke, Nilesh M. Chattar, Dhananjay D. Narsale (2023). A Review on Column Chromatographic Techniques as Separation Method. Iconic Research And Engineering Journals, 6(7).

Dr. Tushar P. Dukre, Sagar S. Sangale, Akash K. Shelke, Nilesh M. Chattar, Dhananjay D. Narsale "A Review on Column Chromatographic Techniques as Separation Method" Iconic Research And Engineering Journals, vol. 6, no. 7, Jan. 2023.

@article{1704002,
      author = {Dr. Tushar P. Dukre, Sagar S. Sangale, Akash K. Shelke, Nilesh M. Chattar, Dhananjay D. Narsale},
      title = {A Review on Column Chromatographic Techniques as Separation Method},
      journal = {Iconic Research And Engineering Journals},
      year = {2023},
      volume = {6},
      number = {7},
      pages = {116-120},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1704002.pdf},
      abstract = {A technique frequently used to extract compounds from complicated mixtures is column chromatography. Column chromatography, as opposed to planar chromatography, packs the stationary phase or polymer (resins) into a column. The packed stationary phase then is passed through the mobile phase toachieve separation. This division may serve analytical or preparatory purposes.
Chromatography, which relies on the interaction of a stationary and a mobile phase, is a crucial method for purifying mixture components.},
      keywords = {Column chromatography, Mobile phase, Stationary phase, Types of column chromatography, etc.},
      month = {January},
  }