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Isolation of Actinomycetes with Antifungal Activities
Subject area: Science,Engineering and Technology · Area of research: Microbiology
Abstract
Three soil samples were taken from three different sites of dense foliage in Makogi, Ogun State. The total actinomycete population isolates from each of the sites was: site A, 2.4 x 104 cfu/g, site B, 2.0 x 105 cfu/g and site C, 1.8 x 103 cfu/g. Fourteen isolates were obtained and of the fourteen, four isolates identified as Streptomyces anulatus (Act 9), Streptomyces xanthophacus (Act 11), Streptomyces noursei (Act 13), and Streptomyces rimosus (Act 14), have antifungal activities. The fungal test strain used when known plants pathogens, Aspergillus niger, Aspergillus flavus, and Fusarium oxysporum. The antifungal zones of inhibition of the isolates ranged from 5 - 25mm. Streptomyces rimosus had the highest antifungal zone of the inhibition of 25mm against A. niger and also had the least zone of inhibition against F. oxysporum. Antifungal inhibition was also more prominent with A. niger and A. flavus than F. oxysporum. The isolate grew well on cellulose agar plates and had zones of clearing which indicated their ability to degrade cellulose.
How to cite this paper
@article{1705814,
author = {Edokpolo Okiemute Bridget},
title = {Isolation of Actinomycetes with Antifungal Activities},
journal = {Iconic Research And Engineering Journals},
year = {2024},
volume = {7},
number = {11},
pages = {692-706},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1705814.pdf},
abstract = {Three soil samples were taken from three different sites of dense foliage in Makogi, Ogun State. The total actinomycete population isolates from each of the sites was: site A, 2.4 x 104 cfu/g, site B, 2.0 x 105 cfu/g and site C, 1.8 x 103 cfu/g. Fourteen isolates were obtained and of the fourteen, four isolates identified as Streptomyces anulatus (Act 9), Streptomyces xanthophacus (Act 11), Streptomyces noursei (Act 13), and Streptomyces rimosus (Act 14), have antifungal activities. The fungal test strain used when known plants pathogens, Aspergillus niger, Aspergillus flavus, and Fusarium oxysporum. The antifungal zones of inhibition of the isolates ranged from 5 - 25mm. Streptomyces rimosus had the highest antifungal zone of the inhibition of 25mm against A. niger and also had the least zone of inhibition against F. oxysporum. Antifungal inhibition was also more prominent with A. niger and A. flavus than F. oxysporum. The isolate grew well on cellulose agar plates and had zones of clearing which indicated their ability to degrade cellulose.},
month = {May},
}