Antibacterial Effect of Vigna Subterranean (Bambara Nut) Leaf Extract on Bacteria Isolated from Wound Swab

References

[1] Adedayo, B., Anyasi, T., Taylor, M., Rautenbach, F., Le Roes-Hill, M., & Jideani, V. (2021). Phytochemical composition and antioxidant properties of methanolic extracts of whole and dehulled Bambara groundnut (Vigna subterranea) seeds. Scientific Reports, 11, 10.1038/s41598-021-93525-w.

[2] Ajiboye, A. A., & Oyejobi, G. K. (2017). In vitro antimicrobial activities of Vigna subterranean. Journal of Antimicrobials Agents, 3, 1–4.

[3] Akbik, D., Ghadiri, M., Chrzanowski, W., & Rohanizadeh, R. (2014). Curcumin as a wound healing agent. Life Sciences, 116(1), 1–7. doi: 10.1016/j.lfs.2014.08.016

[4] Alzohairy, M. A. (2016). Therapeutics Role of Azadirachta indica (Neem) and Their Active Constituents in Diseases Prevention and Treatment. Evidence-Based Complementary and Alternative Medicine, 2016, 7382506. doi: 10.1155/2016/7382506

[5] Anwar, F., Latif, S., Ashraf, M., & Gilani, A. H. (2007). Moringa oleifera: a food plant with multiple medicinal uses. Phytotherapy Research, 21(1), 17–25. doi: 10.1002/ptr.2023

[6] Bădăluță, V. A., Curuțiu, C., Dițu, L. M., Holban, A. M., & Lazăr, V. (2024). Probiotics in Wound Healing. International Journal of Molecular Sciences, 25(11), 5723. doi: 10.3390/ijms25115723

[7] Bassetti, M., Vena, A., & Castaldo, N. (2023). Classification of Wound Infections. In M. Maruccia, G. Papa, E. Ricci, & G. Giudice (Eds.), Pearls and Pitfalls in Skin Ulcer Management (pp. 34). Springer, Cham. doi: 10.1007/978-3-031-45453-0_34

[8] Biswas, K., Chattopadhyay, I., Banerjee, R. K., & Bandyopadhyay, U. (2002). Biological activities and medicinal properties of neem (Azadirachta indica). Current Science, 82(11), 1336–1345. doi: 10.1128/CMR.12.4.564

[9] Bukar, A., & Oyeyi, T. (2010). Antimicrobial profile of Moringa oleifera Lam. extracts against some food-borne microorganisms. Bayero Journal of Pure and Applied Sciences, 3, 10.4314/bajopas.v3i1.58706.

[10] Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology, 94(3), 223–253. doi: 10.1016/j.ijfoodmicro.2004.03.022

[11] Cáceres, A., Cabrera, O., Morales, O., Mollinedo, P., & Mendia, P. (1991). Pharmacological properties of Moringa oleifera. 1: Preliminary screening for antimicrobial activity. Journal of Ethnopharmacology, 33(3), 213–216. doi: 10.1016/0378-8741(91)90078-r

[12] Chen, K., Lin, J. T., Sun, S. B., et al. (2018). Vacuum-assisted closure combined with a closed suction irrigation system for treating postoperative wound infections following posterior spinal internal fixation. Journal of Orthopaedic Surgery and Research, 13, 321. doi: 10.1186/s13018-018-1024-6

[13] Chundran, N., Husen, I., & Rubianti, I. (2015). Effect of Neem Leaves Extract (Azadirachta Indica) on Wound Healing. Althea Medical Journal, 2, 10.15850/amj.v2n2.535.

[14] Costerton, J. W., Stewart, P. S., & Greenberg, E. P. (1999). Bacterial biofilms: a common cause of persistent infections. Science, 284(5418), 1318–1322. doi: 10.1126/science.284.5418.1318

[15] Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582. doi: 10.1128/CMR.12.4.564

[16] Deepak Singh, & Prashant Upadhyay. (2023). Antiinflammatory and Wound Healing Activity of Curcumin containing Phytosomes, Niosomes and Liposomes in rats. Research Journal of Pharmacy and Technology, 16(6), 2776–2778. doi: 10.52711/0974-360X.2023.00456

[17] Diggle, S. P., & Whiteley, M. (2020). Microbe Profile: Pseudomonas aeruginosa: opportunistic pathogen and lab rat. Microbiology (Reading), 166(1), 30–33. doi: 10.1099/mic.0.000860

[18] Ding, X., Tang, Q., Xu, Z., Xu, Y., Zhang, H., Zheng, D., Wang, S., Tan, Q., Maitz, J., Maitz, P. K., Yin, S., Wang, Y., & Chen, J. (2022). Challenges and innovations in treating chronic and acute wound infections: from basic science to clinical practice. Burns & Trauma, 10, tkac014. doi: 10.1093/burnst/tkac014

[19] Garoy, E. Y., Gebreab, Y. B., Achila, O. O., Tekeste, D. G., Kesete, R., Ghirmay, R., Kiflay, R., & Tesfu, T. (2019). Methicillin-Resistant Staphylococcus aureus (MRSA): Prevalence and Antimicrobial Sensitivity Pattern among Patients-A Multicenter Study in Asmara, Eritrea. Canadian Journal of Infectious Diseases and Medical Microbiology, 2019, 8321834. doi: 10.1155/2019/8321834

[20] Gopalakrishnan, L., Doriya, K., & Kumar, D. S. (2016). Moringa oleifera: A Review on Nutritive Importance and Its Medicinal Application. Food Science and Human Wellness, 5, 49–56. doi: 10.1016/j.fshw.2016.04.001

[21] Gould, I. M., & Bal, A. M. (2013). New antibiotic agents in the pipeline and how they can help overcome microbial resistance. Virulence, 4(2), 185–191. doi: 10.4161/viru.22507

[22] Gunes, H., Gulen, D., Mutlu, R., Gumus, A., Tas, T., & Topkaya, A. E. (2016). Antibacterial effects of curcumin: An in vitro minimum inhibitory concentration study. Toxicology and Industrial Health, 32(2), 246–250. doi: 10.1177/0748233713498458

[23] Guo, Y., Song, G., Sun, M., Wang, J., & Wang, Y. (2020). Prevalence and Therapies of Antibiotic-Resistance in Staphylococcus aureus. Frontiers in Cellular and Infection Microbiology, 10, 107. doi: 10.3389/fcimb.2020.00107

[24] Gupta, A., Mahajan, S., & Sharma, R. (2015). Evaluation of antimicrobial activity of Curcuma longa rhizome extract against Staphylococcus aureus. Biotechnology Reports (Amsterdam), 6, 51–55. doi: 10.1016/j.btre.2015.02.001

[25] Hall-Stoodley, L., Costerton, J., & Stoodley, P. (2004). Bacterial biofilms: from the Natural environment to infectious diseases. Nature Reviews Microbiology, 2, 95–108. doi: 10.1038/nrmicro821

[26] Harris, T., & Jideani, V. (2018). Flavonoids and tannin composition of Bambara groundnut (Vigna subterranea) of Mpumalanga, South Africa. Heliyon, 4(9), e00833. doi: 10.1016/j.heliyon.2018.e00833

[27] Harris, T. (2017). Bambara Groundnut (Vigna subterranean) from Mpumalanga Province of South Africa: Phytochemical and Antimicrobial Properties of Seeds and Product Extracts. Doctoral dissertation, Cape Peninsula University of Technology, Bellville, Cape Town, South Africa.

[28] Hassoun, A., Linden, P. K., & Friedman, B. (2017). Incidence, prevalence, and management of MRSA bacteremia across patient populations—a review of recent developments in MRSA management and treatment. Critical Care, 21, 211. doi: 10.1186/s13054-017-1801-3

[29] Hewlings, S. J., & Kalman, D. S. (2017). Curcumin: A Review of Its Effects on Human Health. Foods, 6(10), 92. doi: 10.3390/foods6100092

[30] Ilyas, F., James, A., Khan, S., et al. (2024). Multidrug-Resistant Pathogens in Wound Infections: A Systematic Review. Cureus, 16(4), e58760. doi: 10.7759/cureus.58760

[31] Jideani, V. A., & Jideani, A. I. O. (2021). Phytonutrients and Antioxidant Activity of Bambara Groundnut. In Bambara groundnut: Utilization and Future Prospects (pp. 10). Springer, Cham. doi: 10.1007/978-3-030-76077-9_10

[32] Jideani, V. A., & Diedericks, C. F. (2014). Nutritional, Therapeutic, and Prophylactic Properties of Vigna subterranea and Moringa Oleifera. In O. Oguntibe (Ed.), Antioxidant-Antidiabetic Agents and Human Health (Vol. 9, pp. 187–201). InTech. doi: 10.5772/57169

[33] John, J. E., Carmeli, Y., Cosgrove, S. E., Fowler, V. G., Bronstein, M. Z., Trivette, S. L., Briggs, J. P., Sexton, D. J., & Kaye, K. S. (2003). Adverse Clinical and Economic Outcomes Attributable to Methicillin Resistance among Patients with Staphylococcus aureus Surgical Site Infection. Clinical Infectious Diseases, 36(5), 592–598. doi: 10.1086/367653

[34] Klompong, V., & Benjakul, S. (2015). Antioxidative and Antimicrobial Activities of the Extracts from the Seed Coat of Bambara Groundnut (Voandzeia subterranea). RSC Advances, 5, 9973–9985. doi: 10.1039/C4RA10955D

[35] Kołpa, M., Słowik, R., Wałaszek, M., et al. (2020). Multimodal strategy in surgical site infections control and prevention in orthopaedic patients – a 10-year retrospective observational study at a Polish hospital. Antimicrobial Resistance and Infection Control, 9, 20. doi: 10.1186/s13756-020-0680-6

[36] Lei, J., Sun, L., Huang, S., Zhu, C., Li, P., He, J., Mackey, V., Coy, D. H., & He, Q. (2019). The antimicrobial peptides and their potential clinical applications. American Journal of Translational Research, 11(7), 3919–3931. doi: 10.1128/AAC.45.4.999-1007.2001

[37] Lewis, K. (2001). Riddle of biofilm resistance. Antimicrobial Agents and Chemotherapy, 45(4), 999–1007. doi: 10.1128/AAC.45.4.999-1007.2001

[38] Lin, D. M., Koskella, B., & Lin, H. C. (2017). Phage therapy: An alternative to antibiotics in the age of multi-drug resistance. World Journal of Gastrointestinal Pharmacology and Therapeutics, 8(3), 162–173. doi: 10.4292/wjgpt.v8.i3.162

[39] Maan, P., Yadav, K. S., & Yadav, N. P. (2017). Wound Healing Activity of Azadirachta indica A. Juss Stem Bark in Mice. Pharmacognosy Magazine, 13, S316–S320. doi: 10.4103/0973-1296.210163

[40] Mbikay, M. (2012). Therapeutic Potential of Moringa oleifera Leaves in Chronic Hyperglycemia and Dyslipidemia: A Review. Frontiers in Pharmacology, 3, 24. doi: 10.3389/fphar.2012.00024

[41] Mirghani, R., Saba, T., Khaliq, H., Mitchell, J., Do, L., Chambi, L., Diaz, K., Kennedy, T., Alkassab, K., Huynh, T., Elmi, M., Martinez, J., Sawan, S., & Rijal, G. (2022). Biofilms: Formation, drug resistance and alternatives to conventional approaches. AIMS Microbiology, 8(3), 239-277. doi: 10.3934/microbiol.2022019

[42] Monk, E. J. M., Jones, T. P. W., Bongomin, F., Kibone, W., Nsubuga, Y., Ssewante, N., Muleya, I., Nsenga, L., Rao, V. B., & van Zandvoort, K. (2024). Antimicrobial resistance in bacterial wound, skin, soft tissue and surgical site infections in Central, Eastern, Southern and Western Africa: A systematic review and meta-analysis. PLOS Global Public Health, 4(4), e0003077. doi: 10.1371/journal.pgph.0003077

[43] Mun, S. H., Joung, D. K., Kim, Y. S., Kang, O. H., Kim, S. B., Seo, Y. S., Kim, Y. C., Lee, D. S., Shin, D. W., Kweon, K. T., & Kwon, D. Y. (2013). Synergistic antibacterial effect of curcumin against methicillin-resistant Staphylococcus aureus. Phytomedicine, 20(8-9), 714–718. doi: 10.1016/j.phymed.2013.02.006

[44] Nasrine, A., Narayana, S., Gulzar Ahmed, M., Sultana, R., Noushida, N., Raunak Salian, T., Almuqbil, M., Almadani, M. E., Alshehri, A., Alghamdi, A., Alshehri, S., & Mohammed Basheeruddin Asdaq, S. (2023). Neem (Azadirachta Indica) and silk fibroin associated hydrogel: Boon for wound healing treatment regimen. Saudi Pharmaceutical Journal, 31(10), 101749. doi: 10.1016/j.jsps.2023.101749

[45] Okafor, J. N. C., Meyer, M., Le Roes-Hill, M., & Jideani, V. A. (2022). Flavonoid and Phenolic Acid Profiles of Dehulled and Whole Vigna subterranea (L.) Verdc Seeds Commonly Consumed in South Africa. Molecules, 27(16), 5265. doi: 10.3390/molecules27165265

[46] Oyeyinka, S. A., Abdulsalm, A. O., El-Imam, A. M., Oyeyinka, A. T., Olagunju, O. F., Arise, A. K., Kolawole, F. L., Adedeji, E. O., & Njobeh, P. B. (2021). Total Phenolic Content, Antioxidant, anti-inflammatory and anti-microbial Potentials of Bambara Groundnut (Vigna subterranean L.) Seed Extract. British Food Journal, 0637. doi: 10.1108/BFJ-07-2020-0637

[47] Phan, T. T., See, P., Tran, E., Nguyen, T. T., Chan, S. Y., Lee, S. T., & Huynh, H. (2003). Suppression of insulin-like growth factor signalling pathway and collagen expression in keloid-derived fibroblasts by quercetin: its therapeutic potential use in the treatment and/or prevention of keloids. British Journal of Dermatology, 148, 544–552. doi: 10.1128/AAC.45.4.999-1007.2001

[48] Qin, S., Xiao, W., Zhou, C., Pu, Q., Deng, X., Lan, L., Liang, H., Song, X., & Wu, M. (2022). Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics. Signal Transduction and Targeted Therapy, 7(1), 199. doi: 10.1038/s41392-022-01056-1

[49] Raina, R., Prawez, S., Verma, P., & Pankaj, N. K. (2008). Medicinal Plants and their Role in Wound Healing. VetScan, 3.

[50] Ramatsetse, E., Ramashia, S., & Mashau, M. (2022). A review on health benefits, antimicrobial and antioxidant properties of Bambara groundnut (Vigna subterranean). International Journal of Food Properties, 26, 91–107. doi: 10.1080/10942912.2022.2153864

[51] Rathi, B. S., Bodhankar, S. L., & Baheti, A. M. (2006). Evaluation of aqueous leaves extract of Moringa oleifera Linn for wound healing in albino rats. Indian Journal of Experimental Biology, 44(11), 898–901. doi: 10.1128/AAC.45.4.999-1007.2001

[52] Rockwood, J. L., Anderson, B. G., & Casamatta, D. (2013). Potential uses of Moringa oleifera and an examination of antibiotic efficacy conferred by M. oleifera seed and leaf extracts using crude extraction techniques available to underserved indigenous populations. International Journal of Phototherapy Research, 3, 61–71.

[53] Romani, A., Ieri, F., Turchetti, B., Mulinacci, N., Vincieri, F. F., & Buzzini, P. (2006). Analysis of condensed and hydrolysable tannins from commercial plant extracts. Journal of Pharmaceutical and Biomedical Analysis, 41, 415–420.

[54] Romero, L. C., & Cunha, M. d. L. R. d. S. (2021). Insights into the epidemiology of community-associated methicillin-resistant Staphylococcus aureus in special populations and at the community-healthcare interface. The Brazilian Journal of Infectious Diseases, 25(6), 101636. doi: 10.1016/j.bjid.2021.101636

[55] Rossolini, G. M., Arena, F., Pecile, P., & Pollini, S. (2014). Update on the antibiotic resistance crisis. Current Opinion in Pharmacology, 18, 56–60. doi: 10.1016/j.coph.2014.09.006

[56] Roy, R., Tiwari, M., Donelli, G., & Tiwari, V. (2018). Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action. Virulence, 9(1), 522–554. doi: 10.1080/21505594.2017.1313372

[57] Sidhu, G. S., Mani, H., Gaddipati, J. P., Singh, A. K., Seth, P., Banaudha, K. K., Patnaik, G. K., & Maheshwari, R. K. (1999). Curcumin enhances wound healing in streptozotocin induced diabetic rats and genetically diabetic mice. Wound Repair and Regeneration, 7(5), 362–374. doi: 10.1046/j.1524-475x.1999.00362.x

[58] Silva, N. C. C., & Júnior, A. (2009). Biological properties of medicinal plants: A review of their antimicrobial activity. Journal of Venomous Animals and Toxins including Tropical Diseases, 16, 402–413. doi: 10.1590/S1678-91992010000300006

[59] Sisay, A., Seid, A., Tadesse, S., et al. (2024). Assessment of bacterial profile, antimicrobial susceptibility status, and associated factors of isolates among hospitalized patients at Dessie Comprehensive Specialized Hospital, Northeast Ethiopia. BMC Microbiology, 24, 116. doi: 10.1186/s12866-024-03224-5

[60] Subapriya, R., & Nagini, S. (2005). Medicinal properties of neem leaves: a review. Current Medicinal Chemistry-Anti-Cancer Agents, 5(2), 149–156. doi: 10.2174/1568011053174828

[61] Swanson, T., Keast, D., Bain, K., & Bain, M. (2020). Preventing and treating infection in wounds: translating evidence and recommendations into practice. Wounds International, 11(4). doi: 10.1128/AAC.45.4.999-1007.2001

[62] Taahir, H. (2017). Bambara Groundnut (Vigna subterranean) from Mpumalanga Province of South Africa: Phytochemical and Antimicrobial Properties of Seeds and Product Extracts. MSc Thesis, Cape Peninsula University of Technology.

[63] Thapa, R. K., Kim, J. O., & Kim, J. (2023). Antimicrobial strategies for topical biofilm-based wound infections: past, present, and future. Journal of Pharmaceutical Investigation, 53, 627–641. doi: 10.1007/s40005-023-00628-9

[64] Tiloke, C., Phulukdaree, A., & Chuturgoon, A. A. (2013). The antiproliferative effect of Moringa oleifera crude aqueous leaf extract on cancerous human alveolar epithelial cells. BMC Complementary and Alternative Medicine, 13, 226. doi: 10.1186/1472-6882-13-226

[65] Tomeh, M. A., Hadianamrei, R., & Zhao, X. (2019). A Review of Curcumin and Its Derivatives as Anticancer Agents. International Journal of Molecular Sciences, 20(5), 1033. doi: 10.3390/ijms20051033

[66] Udeh, E. L., Nyila, M. A., & Kanu, S. A. (2020). Nutraceutical and antimicrobial potentials of Bambara groundnut (Vigna subterranean): A review. Heliyon, 6(10), e05205. doi: 10.1016/j.heliyon.2020.e05205

[67] van Walraven, C., & Musselman, R. (2013). The Surgical Site Infection Risk Score (SSIRS): A Model to Predict the Risk of Surgical Site Infections. PLoS One, 8(6), e67167. doi: 10.1371/journal.pone.0067167

[68] Ventola, C. L. (2015). The antibiotic resistance crisis: part 1: causes and threats. P T, 40(4), 277–283. doi: 10.1128/AAC.45.4.999-1007.2001

[69] Verma, A. R., Vijayakumar, M., Mathela, C. S., & Rao, C. V. (2009). In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves. Food and Chemical Toxicology, 47(9), 2196–2201. doi: 10.1016/j.fct.2009.06.005

[70] Wanyama, A. W., Orwa, J. A., Njenga, P. K., & Irungu, B. N. (2017). Evaluation of cytotoxicity, antimicrobial activities and minerals composition of Vigna subterranea (L.) Verdc. (Bambara Groundnut) extracts. African Journal of Health Sciences, 30(2), 88–104. doi: 10.1128/AAC.45.4.999-1007.2001

[71] Wanyama, A. W. (2018). Evaluation of Phytoconstituents, Antioxidants Potential, Cytotoxic, Antimicrobial Activities and Mineral Composition of Vigna subterranea (L) Verdic. Extracts. Doctoral dissertation, JKUAT–COHES.

[72] Wanyama, A. W., Orwa, J. A., Njenga, P. K., & Irungu, B. N. (2017). Evaluation of Cytotoxicity, Antimicrobial Activities and Minerals Composition of Vigna subterranea (L.) Verdc. (Bambara Groundnut) Extracts. African Journal of Health Sciences, 30, 88–104.

[73] Yousefian, F., Hesari, R., Jensen, T., Obagi, S., Rgeai, A., Damiani, G., Bunick, C. G., & Grada, A. (2023). Antimicrobial Wound Dressings: A Concise Review for Clinicians. Antibiotics (Basel), 12(9), 1434. doi: 10.3390/antibiotics12091434

[74] Zhang, Q. Y., Yan, Z. B., Meng, Y. M., Hong, X. Y., Shao, G., Ma, J. J., Cheng, X. R., Liu, J., Kang, J., & Fu, C. Y. (2021). Antimicrobial peptides: mechanism of action, activity and clinical potential. Military Medical Research, 8(1), 48. doi: 10.1186/s40779-021-00343-2

@article{1706449,
      author = {Silas Kelechi Henry, Anorue Stanley Chukwuchebem, Ndubueze Winners Chizaram},
      title = {Antibacterial Effect of Vigna Subterranean (Bambara Nut) Leaf Extract on Bacteria Isolated from Wound Swab},
      journal = {Iconic Research And Engineering Journals},
      year = {2024},
      volume = {8},
      number = {4},
      pages = {478-498},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1706449.pdf},
      abstract = {Wound infections pose a significant global health challenge, exacerbated by the alarming rise of multidrug-resistant (MDR) bacteria. The increasing ineffectiveness of conventional antibiotics necessitates the exploration of alternative therapeutic strategies, particularly those derived from natural sources. This research investigates the antibacterial properties of Vigna subterranea (Bambara nut) leaf extract against common wound-infecting bacteria, focusing on MDR strains. The study employed a comprehensive approach, including phytochemical analysis, antibacterial testing using the disk diffusion and microdilution methods, and a comparative analysis against standard antibiotics. Vigna subterranea leaf extract demonstrated moderate antibacterial activity against Staphylococcus aureus and Escherichia coli, with MIC values ranging from 500 ?g/mL to 600 ?g/mL. The extract exhibited limited effectiveness against Pseudomonas aeruginosa. Phytochemical analysis revealed the presence of bioactive compounds such as flavonoids, tannins, saponins, and alkaloids, suggesting their potential contribution to the observed antibacterial properties. These findings highlight the potential of Vigna subterranea leaf extract as a natural antibacterial agent for wound management, particularly against Staphylococcus aureus. The extract's safe profile, broad availability, and potential synergistic effects with existing treatments make it a promising candidate for development as a topical wound treatment. Further research is crucial to elucidate the mechanisms of action, optimize its therapeutic potential, and establish its clinical relevance through in vivo and clinical trials. This research contributes to the growing body of knowledge on plant-based antimicrobial agents, providing a potential solution to the critical challenge of antibiotic resistance in wound infections. The exploration of Vigna subterranea leaf extract offers a promising avenue for developing sustainable and accessible treatment options for wound management, particularly in resource-limited settings.},
      month = {October},
  }