Optimization of Bioethanol Production from Cassava Wastewater Using Response Surface Methodology

References

[1] Amoikon, T. L. S., Aké, M. D. F., Djéni, N. T., Grondin, C., Casaregola, S., & Djè, K. M. (2019). Diversity and enzymatic profiles of indigenous yeasts isolated from three types of palm wines produced in Côte d'Ivoire. Journal of Applied Microbiology, 126(2), 567-579.

[2] Antia, O., Olosunde, W., & Ekong, E. (2021). Investigation into some physicochemical properties of cassava processing effluent. International Research Journal of Advanced Engineering and Science, 6(2), 134-137.

[3] Antia, U. E., Akan, O. D., Stephen, N. U., Eno-Ibanga, C. K., & Akpan, N. G. (2018). Isolation and Screening of Yeast Isolates Indigenous Palm Wine for Ethanol Production. Philippine Journal of Science, 147(3).

[4] Archibong, E. J., Ifeanyi, E. O., Okafor, O. I., Okafor, U. I., Ezewuzie, C. S., Ezembor, G. C., & Anakwenze, V. N. (2016). Ethanol production from cassava wastes (pulp and peel) using alcohol-tolerant yeast isolated from palm wine. American Journal of Life Science Research, 4(3), 92-97.

[5] Bartošová, A., & Blinová, L. (2017). Study of Wastewaters Contaminated with Heavy Metals in Bioethanol Production. Research Papers Faculty of Materials Science and Technology Slovak University of Technology, 25(40), 63-71.

[6] Chantawan, N., Moungprayoon, A., Lunprom, S., Reungsang, A., & Salakkam, A. (2022). High-solid dark fermentation of cassava pulp and cassava processing wastewater for hydrogen production. International Journal of Hydrogen Energy, 47(96), 40672-40682.

[7] Chidubem-Nwachinemere, N. O., Orji, M. U., Achugbu, A. N., Achugbu, O. E., Okoli, U. O., Chidimma, O., & Ozioma, N. A. (2023). Physicochemical Composition of Cassava Mill Effluents in Five Processing Plants in Anambra State, Nigeria. World Wide Journal of Multidisciplinary Research and Development, 9(05), 31-34.

[8] de Gois Araújo Tavares, T., Peiter, F. S., Chaves, T. C., Gois, G. N. S. B., & de Amorim, E. L. C. (2023). Effect of thermophilic temperatures on hydrogen and ethanol production in anaerobic fluidised bed reactor from cassava wastewater. Brazilian Journal of Chemical Engineering, 40(1), 115-127.

[9] Ejimofor, C. F., Nnamdi, N., Odira, J., Chikaodili, A. E., & Onyinye, M. A. (2023). Isolation and characterisation of yeast associated with palm wine fermentation. Asian Journal of Food Research and Nutrition, 2(3), 25-35.

[10] Ekop, I. E., Simonyan, K. J., & Evwierhoma, E. T. (2019). Utilization of cassava wastes for value-added products: An overview. International Journal of Scientific Engineering and Science, 3(1), 31-39.

[11] Ejimofor Chiamaka Frances, E., Nnamdi Enoch, N., Odira Johnson, O., Chikaodili Eziamaka, A. E., & Onyinye Ann, M. (2023). Isolation and characterisation of yeast associated with palm wine fermentation. Asian Journal of Food Research and Nutrition, 2(3), 25-35.

[12] Ezeigbo, O. R., Ukabi, C. F., Ike-Amadi, C. A., & Ekaiko, M. U. (2015). Determination of starch and cyanide contents of different species of fresh cassava tuber in Abia State, Nigeria. British Biotechnology Journal, 6(1), 10.

[13] Frances, C. E., Nnamdi Enoch, N., Odira Johnson, O., Chikaodili Eziamaka, A. E., & Onyinye Ann, M. (2023). Isolation and characterisation of yeast associated with palm wine fermentation. Asian Journal of Food Research and Nutrition, 2(3), 25-35.

[14] Ferraro, V., Piccirillo, C., Tomlins, K., & Pintado, M. E. (2016). Cassava (Manihot esculenta Crantz) and yam (Dioscorea spp.) crops and their derived foodstuffs: safety, security and nutritional value. Critical reviews in food science and nutrition, 56(16), 2714-2727.

[15] Gilmore, K. R., & Luong, H. V. (2016). Improved method for measuring total dissolved solids. Analytical Letters, 49(11), 1772-1782.

[16] Hawaz, E., Tafesse, M., Tesfaye, A., Beyene, D., Kiros, S., Kebede, G., ... & Muleta, D. (2022). Isolation and characterisation of bioethanol-producing wild yeasts from bio-wastes and co-products of sugar factories. Annals of Microbiology, 72(1), 39.

[17] Ikuemonisan, E. S., Mafimisebi, T. E., Ajibefun, I., & Adenegan, K. (2020). Cassava production in Nigeria: trends, instability and decomposition analysis (1970–2018). Heliyon, 6(10), eo5089.

[18] Izah, S. C., Bassey, S. E., & Ohimain, E. I. (2018). Impacts of cassava mill effluents in Nigeria. Journal of Plant and Animal Ecology, 1(1), 14-42.

[19] Kotoka, F., Tulashie, S. K., & Setsoafia, D. D. (2017). Production of bioethanol from liquid waste from cassava dough during gari processing. Biofuels, 10(4), 493–501.

[20] Niyomvong, N. (2019). Ethanol production from cassava stem using Saccharomyces cerevisiae TISTR 5339 through simultaneous saccharification and fermentation. Agriculture and Natural Resources, 53(6), 667-673.

[21] Nizzy, A. M., & Kannan, S. (2022). A review on the conversion of cassava wastes into value-added products towards a sustainable environment. Environmental Science and Pollution Research, 29(46), 69223-69240.

[22] Olaoye, R. A., Afolayan, O. D., Adeyemi, K. A., Ajisope, L. O., & Adekunle, O. S. (2020). Adsorption of selected metals from cassava processing wastewater using cow-bone ash. Scientific African, 10, e00653.

[23] Olaniyi, O. O., Akinlami, O. R., Adeleke, B. S., Alabi, G. O., & Akinyele, B. J. (2019a). Isolation and characterisation of alcohol-tolerant Saccharomyces cerevisiae from palm wine (Raffia palm). Annals of Food Science and Technology, 20(4), 701-709.

[24] Olaniyi, O. S., Donkor, N. K., & Adebayo, A. A. (2019b). Impact of cassava-based feed on the gut health of fish: A review. Aquaculture Reports, 17, 100215.

[25] Otekunrin, O. A., & Sawicka, B. (2019). Cassava, a 21st-century staple crop: How can Nigeria harness its enormous trade potentials. Acta Scientific Agriculture, 3(8), 194-202.

[26] Onyediako, P. O., & Adiele, J. G. (2022). Enhanced Cassava Production for Food Security and Economic Development in Nigeria: A review. Nigeria Agricultural Journal, 53(3), 204-211.

[27] Oghenejoboh, K. M., Orugba, H. O., Oghenejoboh, U. M., & Agarry, S. E. (2021). Value added cassava waste management and environmental sustainability in Nigeria: A review. Environmental Challenges, 4, 100127.

[28] Pothiraj, C., Arun, A., & Eyini, M. (2015). Simultaneous saccharification and fermentation of cassava waste for ethanol production. Biofuel Research Journal, 2(1), 196-202.

[29] Saggi, S. K., & Dey, P. (2019). An overview of simultaneous saccharification and fermentation of starchy and lignocellulosic biomass for bio-ethanol production. Biofuels, 10(3), 287-299.

[30] Srimuang, T., & Polprasert, S. (2019). The utilisation of cassava pulp and cassava wastewater for bioethanol production. RMUTP Research Journal, 13(2), 167-175.

[31] Sukasem, N., Hareemao, T., & Sudawong, C. (2017). The mimic of fractional distillation technology for the development of a homegrown pot distillery for ethanol distillation. Energy Procedia, 138, 985-990.

[32] Ugwuodo, C. B., Anike, E. N., Ukochukwu, H., & Nwosu-Obieogu, K. (2021). Optimisation of process parameters in ethanol production from cassava waste slurry. LAUTECH Journal of Engineering and Technology, 15(2), 10–20.

[33] Umo, A. M., Egemba, K. C., Bassey, E. N., & Etuk, B. R. (2013). Optimisation of the ethanol fermentation of cassava wastewater using the response surface method. Global Journal of Engineering Research, 12, 13–23.

[34] Zhang, P., Hai, H., Sun, D., Yuan, W., Liu, W., Ding, R., & Chen, C. (2019). A high throughput method for total alcohol determination in fermentation broths. BMC Biotechnology, 19, 1-8.

[35] Zheng, T., Lei, F., Li, P., Han, C., Liu, S., & Jiang, J. (2019). High rhamnolipid production from cassava residues by simultaneous saccharification and fermentation with low enzyme loading. Journal of Biobased Materials and Bioenergy, 13(5), 635-642.

@article{1706833,
      author = {Amaoma J. N, Onwukwe, C. D., Stanley, H. O.},
      title = {Optimization of Bioethanol Production from Cassava Wastewater Using Response Surface Methodology},
      journal = {Iconic Research And Engineering Journals},
      year = {2025},
      volume = {8},
      number = {7},
      pages = {14-33},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1706833.pdf},
      abstract = {Nigeria, a leading global producer of cassava, generates substantial amounts of processing waste, including wastewater. This readily available feedstock presents an opportunity to address the environmental challenges associated with improper waste disposal. Cassava wastewater, if not managed effectively, can contaminate water sources and contribute to soil degradation. This study investigated the optimization of bioethanol production from cassava wastewater using Response Surface Methodology (RSM). Four independent variables, namely temperature (25-45 ?C), pH (6-8), incubation time (3-7 days), and wastewater concentration (25-100%), were evaluated using a Central Composite Design. A two-factor interaction (2FI) model was found to be statistically significant (p < 0.0001), with an adjusted R? of 0.9463 and a predicted R? of 0.8809, indicating a good fit. The model predicted maximum ethanol yield (17.34% v/v) at 45 ?C, pH 8, 6 days? incubation, and 100% wastewater concentration. Validation experiments confirmed the model's accuracy, with an observed ethanol yield of 16.50% ? 0.87% (v/v), which was within the 95% prediction interval. Concurrent analysis revealed significant reductions in heavy metal concentrations (e.g., lead from 0.032 mg/L to 0.0021 mg/L) and total solids (from 119.65 mg/L to 3.71 mg/L) in the wastewater after fermentation. These findings demonstrate the feasibility of bioethanol production from cassava wastewater, offering a sustainable solution for waste management and a promising avenue for renewable energy generation.},
      keywords = {Bioethanol, cassava, waste water, palm wine, saccharomyces cerevisae, RSM, Optimisation, and renewable energy},
      month = {January},
  }