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Sesquiterpenes: The Major Bioactive Constituent of the Nigerian Chromolaena odorata (Siam Weed) Leaf Extracts
Subject area: Science,Engineering and Technology · Area of research: Natural Products Chemistry
Abstract
Employing phytochemicals from medicinal plants in drug synthesis is crucial since drug-resistant infections and diseases pose serious threat to life. This study investigated the chemical composition and antimicrobial activity of the Nigerian Chromolaena odorata based on its ethnomedical use. The crude extract was obtained by extracting the air-dried, powdered leaves of Chromolaena odorata using methanol. Using standard procedures, the extract was tested for phytochemicals. The fractions of the crude extract that were soluble in hexane, chloroform, and ethylacetate were separated. The activity of the crude extract and its fractions against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Candida albicans was assessed. By using GC-MS analysis, the chemical components of the chloroform-soluble fraction that exhibited the strongest inhibitory activity were identified. The phytochemical screening showed that saponins and glycosides were absent, but terpenoids, alkaloids, tannins, steroids/sterols, and volatile oils were present. The gram-negative bacterium E. coli was insensitive to the crude extract and fractions, while they showed inhibitory activity against the gram-positive bacteria S. aureus and B. subtilis. The only extracts that showed activity against Candida albicans were the ethylacetate and chloroform soluble fractions. Terpenes with known biological activity were the primary compounds identified by GC-MS analysis of the bioactive chloroform-soluble fraction extract. Terpenes may be responsible for the plant extracts' action. These results support the traditional use of Chromolaena odorata and show its potential as a natural source of antimicrobial compounds.
Keywords
Antimicrobial Activity, Chromolaena Odorata, Phytochemicals, Sesquiterpenes
References
[1] Newman, D. J., Craggs, G. M. (2012). Natural Products as Sources of New Drugs Over the 30 years from 1981 to 2010. Journal of Natural Products, 75(3), 311-335.
[2] Harvey, A. L., Edrada-Ebel, R., Quinn, R. J., & Robertson, L. P. (2015). The role of Natural Products in Drug Discovery and Development in the next Millennium. Natural Product Reports, 32(3), 394-426.
[3] Gurib-Fakim, A. (2016). Medicinal plants: Traditions of Yesterday and Drugs of Tomorrow. Molecular Aspects of Medicine, 27(1), 1-93.
[4] Patel, A., Kumar, V., Chakraborty, D., Singh, N., & Chakraborty, S. (2007). Purification and characterization of an antifungal compound produced by Bacillus licheniformis BC98, and its antagonistic effects on Fusarium oxysporum. Journal of Microbiology and Biotechnology, 17(2), 193-203.
[5] Adedapo, A. A., & Jimoh, F. O. (2018). Ethnobotanical Survey of Chromolaena odorata (L.) King & H. E. Robins (Asteraceae) in Nigeria. International Journal of Plant & Soil Science, 25(1), 1-10.
[6] Phetburom, N., Chopjitt, P., Dulyasucharit, R., Nontunha, N., Daenprakhom, K., Ongarj, P., ... & Boueroy, P. (2025). Antimicrobial activity of Chromolaena odorata crude extracts against Streptococcus suis. Microbial Pathogenesis, 107799.
[7] Thophon, S. H. S., Waranusantigul, P., Kangwanrangsan, N., & Krajangsang, S. (2016). Antimicrobial activity of Chromolaena odorata extracts against bacterial human skin infections. Modern Applied Science, 10(2).
[8] Obi, L. K., & Okwute, S. K. (2023). Chemical and Biological Investigations of the Leaf Extracts of Andrographis paniculata (Acanthaceae). Journal ISSN, 2766, 2276.
[9] Juliani Jr, H. R., Biurrun, F., Koroch, A. R., Oliva, M. M., Demo, M. S., Trippi, V. S., & Zygadlo, J. A. (2002). Chemical constituents and antimicrobial activity of the essential oil of Lantana xenica. Planta Medica, 68(08), 762-764.
[10] Ngu, T. N., Hanh, D. T., Trong, P. V., Bao, P. H., Hien, N. T., My, N. Q., ... & To, D. C. (2024). Chemical Composition, Biological Activities, and Docking Studies of Essential Oil from Eupatorium odoratum L. Collected in Dak Lak, Vietnam. Tropical Journal of Natural Product Research, 8(11).
[11] Araujo, F. M., Dantas, M. C., e Silva, L. S., Aona, L. Y., Tavares, I. F., & de Souza-Neta, L. C. (2017). Antibacterial activity and chemical composition of the essential oil of Croton heliotropiifolius Kunth from Amargosa, Bahia, Brazil. Industrial crops and products, 105, 203-206.
How to cite this paper
@article{1710173,
author = {Kayode Confidence Praise, Obi Leonard Kelechukwu, Adewumi Chizoma Nwakego, Aboh Sandra Ojochegbe},
title = {Sesquiterpenes: The Major Bioactive Constituent of the Nigerian Chromolaena odorata (Siam Weed) Leaf Extracts},
journal = {Iconic Research And Engineering Journals},
year = {2025},
volume = {9},
number = {2},
pages = {435-443},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1710173.pdf},
abstract = {Employing phytochemicals from medicinal plants in drug synthesis is crucial since drug-resistant infections and diseases pose serious threat to life. This study investigated the chemical composition and antimicrobial activity of the Nigerian Chromolaena odorata based on its ethnomedical use. The crude extract was obtained by extracting the air-dried, powdered leaves of Chromolaena odorata using methanol. Using standard procedures, the extract was tested for phytochemicals. The fractions of the crude extract that were soluble in hexane, chloroform, and ethylacetate were separated. The activity of the crude extract and its fractions against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Candida albicans was assessed. By using GC-MS analysis, the chemical components of the chloroform-soluble fraction that exhibited the strongest inhibitory activity were identified. The phytochemical screening showed that saponins and glycosides were absent, but terpenoids, alkaloids, tannins, steroids/sterols, and volatile oils were present. The gram-negative bacterium E. coli was insensitive to the crude extract and fractions, while they showed inhibitory activity against the gram-positive bacteria S. aureus and B. subtilis. The only extracts that showed activity against Candida albicans were the ethylacetate and chloroform soluble fractions. Terpenes with known biological activity were the primary compounds identified by GC-MS analysis of the bioactive chloroform-soluble fraction extract. Terpenes may be responsible for the plant extracts' action. These results support the traditional use of Chromolaena odorata and show its potential as a natural source of antimicrobial compounds.},
keywords = {Antimicrobial Activity, Chromolaena Odorata, Phytochemicals, Sesquiterpenes},
month = {August},
}