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Screening for Drought Tolerance in Cowpea (Vignaungiculata(L) Walp) At Seedling and Flowering Stages Under Screen House Condition: Selecting for Recovery Ability Traits

Abimiku, Obed Enab Ndor, Emmanuel Aliyu, Daniel

Subject area: Science,Engineering and Technology  ·  Area of research: Agriculture

DOI: 10.64388/IREV9I4-1711450-5354

Abstract

Prolonged drought of cowpea at the seedling stage can affect survival and productivity. One of the most important screening techniques used in cowpea selection for drought tolerance is screening at the seedling and flowering stages. This study sought to evaluate cowpea genotypes for their resistance to seedling and flowering stages during drought stress. Twenty four accessions of cowpea (Vigna unguiculata L. Walp) were collected from the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria and Seed center, University of Agriculture, Makurdi to evaluated for their drought tolerance at seedling stages at preliminary trial. Fourteen were selected as the accessions proved to be tolerant and were further used The experiment was laid out in a Completely Randomized Design (CRD) with three replications at the screen house of the Department of Crop Production Technology of College of Agriculture, Science and Technology Lafia, Nasarawa stata, Nigeria. Drought stress was imposed for 3 and 5 weeks after the full expansion of the first trifoliate leaf and first flower initiation. Soil Plant Analysis Development (SPAD) chlorophyll meter readings of unifoliate, first, and second trifoliate leaves of each genotype was measured on a weekly basis due to the predictive ability of chlorophyll content on drought tolerance. Analysis of variance (ANOVA) revealed high significant differences among accessions for wilting and recovery traits, stomatal conductance, relative water content (RWC), terminal leaflet length (TLL) and width (TLW), stem girth, and yield parameters under drought stress. AC02, AC04, AC07 and AC14 were observed to be highly susceptible. AC04, ACO6 and AC11 were moderately susceptible. AC03 and AC09 were moderately tolerant, while AC10 and AC12 were the highly tolerant accessions. The moderately tolerant and the highly tolerant accessions showed a combination of superior resistance to wilting, superior recovery rates, and superior yield attributes. Accessions that showed high tolerance and great variability can be used as parents in future for breading programmes.

Keywords

Cowpea Accessions, Vigna ungiculata, drought tolerance, Recover traits and Heritability.

References

[1] Abimiku, O.E, Bello, L.L, Omoiugui, L Vange T. (2020). Combining Ability and Heterosis for Grain Yield and Yield Related Component in Maize Resistant to Striga Hermonthica (Del) Benth in Southern Guinea Savannah of Nigeria. World Journal of Innovative Research, (WJIR) ISSN: 2454- 8236. Vol.8- Issue 3, March 2020. Pages 42-48

[2] Abdou Razakou, I.B.Y., Mensah, B., Addam, K.S., Akromah, R. 2013. Using morpho– physiological parameters to evaluate cowpea varieties for drought tolerance, International Journal of Agricultural Science Research, 2(5): 153–162.

[3] Agbicodo, E.M., Fatokun, C.A., Muranaka, S., Visser, R.G.F., Linden van der, C.G. 2009. Breeding drought-tolerant cowpea: constraints, accomplishments, and future prospects, Euphytica, 167: 353–370.

[4] Ajayi, A.T., Gbadamosi, A.E., Olumekun, V.O. 2017b. Correlation and principal component analyses of important traits of cowpea seedlings under drought stress. 41st Genetics Society of Nigeria Conference, 15–19 October, p. 314–321.

[5] Ajayi, A.T., Gbadamosi, A.E., Olumekun, V.O. 2018. Screening for Drought Tolerance in Cowpea [Vigna unguiculata (L.) Walp] at the seedling stage under screen house condition, International Journal of BioSciences and Technology, 11(1): 1–19.

[6] Ajayi, A.T. 2020. Relationships among drought tolerance indices and yield characters of cowpea [Vigna unguiculata (L.) Walp], International Journal of Scientific Research in Biological Sciences, 7(5): 93–103.

[7] Ajayi, A.T., Gbadamosi, A.E., Osekita, O.S., Taiwo, B.H., Fawibe, A. B., Adedeji, I., Omisakin, T. 2022. Genotype × environment interaction and adaptation of cowpea genotypes across six planting seasons, Frontiers in Life Sciences and Related Technologies, 3: 7–15. https://doi.org/10.51753/flsrt.1036051

[8] Alidu, M.S., Asante, I.K., Tongoona, P., Ofori, K., Danquah, A., Padi, F.K. 2019. Development and screening of cowpea recombinant inbred lines for seedling drought tolerance, Journal of Plant Breeding and Crop Science, 11(1): 1–10. https://doi.org/10.5897/jpbcs2018.0768

[9] Al-Naggar, A.M.M., Soliman, S.M., Hashimi, M.N. 2011. Tolerance to drought at flowering stage of 28 maize hybrids and populations, Egyptian Journal of Plant Breeding, 15(1): 69– 87.

[10] Badu-Apraku, B., Obesesan, O., Abiodun, A., Obeng-bio, E. 2021. Genetic gains from selection for drought tolerance during three breeding periods in extra-early maturing maize hybrids under drought and rainfed environments, Agronomy, 11(831). https://doi.org/10.3390/ agronomy11050831

[11] Ezin, V., Gloria, A., Tosse, C., Chabi, B., Ahanchede, A. 2021. Adaptation of cowpea [Vigna unguiculata (L.) Walp] to water deficit during vegetative and reproductive phases using physiological and agronomic characters, International Journal of Agronomy, 2021(96653122021). https://doi.org/10.1155/2021/9665312

[12] Garg, B.K., Burman, U., Kathju, S. 2005. Comparative water relations, photosynthesis and nitrogen metabolism of arid legumes under water stress, Journal of Plant Biology, 32: 83– 93.

[13] Gomes AMF, Nhantumbo N, Ferreira-Pinto M, Massinga R, Ramalho JC, Ribeiro-Barros A. Breeding E ite Cowpea [Vigna unguiculata (L.) Walp] Varieties for Improved Food Security and Income in Africa: Opportunities and Challenges. IntechOpen. 2019; doi: dx.doi.org/10.5772/intechopen.84985.

[14] Hall AE, Cisse N, Thiaw S, Elawad HOA, Ehlers JD, Ismail AM, Fery RL, Roberts PA, Kitch LW, Murdock LL, Boukar O, Phillips RD, McWatters KH. Development of Cowpea Cultivars and Germplasm by the Bean/Cowpea CRSP. Field Crops Research. 2003; 82:103–134.

[15] Harshani, H. K. A., Fernando, K.M.C. 2021. Root system attributes, morphology, and yield of cowpea [Vigna unguiculata (L.) Walp] under moisture stress, Tropical Agricultural Research and Extension, 24(3). https://doi.org/10.4038/tare.v24i3.5512

[16] Ibitoye, D.O. (2015). Genetic analysis of drought tolerance in cowpea [Vigna unguiculata (L.) Walp]. Ph.D. Thesis. School of Agriculture, University of Ghana, Legon, Ghana.

[17] IBPGR. 1983. International board for plant genetic resources cowpea descriptors. Rome, Italy.

[18] Mathews, R.B., Azam-Ali, S.N., Peacock, J.M. 1990. Response of four sorghum lines to midseason drought, Field Crops Responses, 5: 297–308.

[19] Nkouannessi, M. 2005. The genetic, morphological, and physiological evaluation of African cowpea genotypes. MSc Dissertation. University of Free State, Faculty of Natural and Agricultural Sciences, Bloemfontein, South Africa.

[20] Nunes, C., Moreira, R., Pais, I., Semedo, J., Sim, F., Veloso, M.M., Scotti-campos, P. 2022. Cowpea physiological responses to terminal drought–comparison between four landraces and a commercial variety, Plants, 11: 593. https://doi.org/10.3390/ plants110505

[21] Ogbaga, C.C., Stepien, P., Johnson, G.N. 2014. Sorghum (Sorghum bicolor) varieties adopt strongly contrasting strategies in response to drought, Physiologia Plantarum, https://doi.org/10.1111/ppl.12196

[22] Pungulani, L.M., James, P.M., Warren, M.W., Mackson, B. 2013. Improvement of leaf wilting scoring system in cowpea [Vigna unguiculata (L.) Walp]: from qualitative scale to quantitative index, Australian Journal of Crop Science, 7(9): 1262–1269.

[23] Ravelombola W, Shi A,, Qin J, Weng Y, Bhattarai G, Zia B, Zhou W, and Mou B. Investigation on Various Aboveground Traits to Identify Drought Tolerance in Cowpea Seedlings. American Society for Horticultural Science.2018; 53:1757-1765. https://doi.org/10.21273/HORTSCI13278-18.

[24] Santos, R., Carvalho, M., Rosa, E., Carnide, V., Castro, I. 2020. Root and agro-morphological traits performance in cowpea under drought stress, Agronomy, 10: 1604. http://dx.doi.org/10.3390/agronomy10101604

[25] Shanmugam, S., Boyett, V.A., Id, M.K. 2021. Enhancement of drought tolerance in rice by silencing of the OsSYT-5 gene, PlosOne, 16(10): e0258171.

[26] Singh SK, Kakani VG, Surabhi GK, Reddy KR. Cowpea (Vigna unguiculata [L.] Walp.) Genotypes Response to Multiple Abiotic Stresses. Journal of Photochemistry and Photobiology. 2010; 100:135-146. doi: 10.1016/j. jphotobiol.2010.05.013.

[27] Singh, C.M., Singh, P., Tiwari, C., Purwar, S., Kumar, M., Pratap, A., Singh, S., Chugh, V., Mishra, A.K. 2021. Improving drought tolerance in mungbean [Vigna radiata (L.) Wilczek]: morpho-physiological, biochemical, and molecular perspectives, Agronomy, 11: 1534. https://doi.org/10.3390/ agronomy11081534

[28] Watanabe, I. S., Hakoyama, T., Terao, T., Singh B. B. (1997). Evaluation methods for drought tolerance in cowpea. In: Advances in cowpea research Eds. B. B. Singh, D. R. Mohan Raj, K. E. Dashiell, Jackai L. E N. Copublication of International Institute of Tropical Agriculture (IITA) and Japan International Research Centre for Agricultural Sciences (JIRCAS), Ibadan, Nigeria, Pp 142 – 146.

[29] Wasae, A. 2021. Evaluation of drought stress tolerance based on selection indices in haricot bean varieties exposed to stress at different, International Journal of Agronomy, 2021: 6617874. https://doi.org/10.1155/2021/6617874

How to cite this paper

Abimiku, Obed Enab, Ndor, Emmanuel, Aliyu, Daniel "Screening for Drought Tolerance in Cowpea (Vignaungiculata(L) Walp) At Seedling and Flowering Stages Under Screen House Condition: Selecting for Recovery Ability Traits" Iconic Research And Engineering Journals Volume 9 Issue 4 2025 Page 1228-1241 https://doi.org/10.64388/IREV9I4-1711450-5354
Abimiku, Obed Enab, Ndor, Emmanuel, Aliyu, Daniel "Screening for Drought Tolerance in Cowpea (Vignaungiculata(L) Walp) At Seedling and Flowering Stages Under Screen House Condition: Selecting for Recovery Ability Traits" Iconic Research And Engineering Journals, vol. 9, no. 4, Oct. 2025, doi: https://doi.org/10.64388/IREV9I4-1711450-5354
Abimiku, Obed Enab, Ndor, Emmanuel, Aliyu, Daniel (2025). Screening for Drought Tolerance in Cowpea (Vignaungiculata(L) Walp) At Seedling and Flowering Stages Under Screen House Condition: Selecting for Recovery Ability Traits. Iconic Research And Engineering Journals, 9(4). doi: https://doi.org/10.64388/IREV9I4-1711450-5354
Abimiku, Obed Enab, Ndor, Emmanuel, Aliyu, Daniel "Screening for Drought Tolerance in Cowpea (Vignaungiculata(L) Walp) At Seedling and Flowering Stages Under Screen House Condition: Selecting for Recovery Ability Traits" Iconic Research And Engineering Journals, vol. 9, no. 4, Oct. 2025. Crossref, https://doi.org/10.64388/IREV9I4-1711450-5354
@article{1711450,
      author = {Abimiku, Obed Enab, Ndor, Emmanuel, Aliyu, Daniel},
      title = {Screening for Drought Tolerance in Cowpea (Vignaungiculata(L) Walp) At Seedling and Flowering Stages Under Screen House Condition: Selecting for Recovery Ability Traits},
      journal = {Iconic Research And Engineering Journals},
      year = {2025},
      volume = {9},
      number = {4},
      pages = {1228-1241},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1711450.pdf},
      abstract = {Prolonged drought of cowpea at the seedling stage can affect survival and productivity. One of the most important screening techniques used in cowpea selection for drought tolerance is screening at the seedling and flowering stages. This study sought to evaluate cowpea genotypes for their resistance to seedling and flowering stages during drought stress.  Twenty four accessions of cowpea (Vigna unguiculata L. Walp) were collected from the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria and Seed center, University of Agriculture, Makurdi to evaluated for their drought tolerance at seedling stages at preliminary trial. Fourteen were selected as the accessions  proved to be tolerant and were further used  The experiment was laid out in a Completely Randomized Design (CRD) with three replications at the screen house of the Department of Crop Production Technology of College of Agriculture, Science and Technology Lafia, Nasarawa stata, Nigeria. Drought stress was imposed for 3 and 5 weeks after the full expansion of the first trifoliate leaf and first flower initiation. Soil Plant Analysis Development (SPAD) chlorophyll meter readings of unifoliate, first, and second trifoliate leaves of each genotype was measured on a weekly basis due to the predictive ability of chlorophyll content on drought tolerance. Analysis of variance (ANOVA) revealed high significant differences among accessions for wilting and recovery traits, stomatal conductance, relative water content (RWC), terminal leaflet length (TLL) and width (TLW), stem girth, and yield parameters under drought stress. AC02, AC04, AC07 and AC14 were observed to be highly susceptible. AC04, ACO6 and AC11 were moderately susceptible. AC03 and AC09 were moderately tolerant, while AC10 and AC12 were the highly tolerant accessions. The moderately tolerant and the highly tolerant accessions showed a combination of superior resistance to wilting, superior recovery rates, and superior yield attributes. Accessions that showed high tolerance and great variability can be used as parents in future for breading programmes.},
      keywords = {Cowpea Accessions, Vigna ungiculata, drought tolerance, Recover traits and Heritability.},
      month = {October},
      doi = {https://doi.org/10.64388/IREV9I4-1711450-5354}
  }