Occurrence of Carbapenem – Resistant Escherichia Coli Carrying Bla NDM and Bla Kpc Genes from Commericlal Poultry Farm Environments in Aba Abia State.
Abstract
Carbapenem antibiotics are the referred to as the last resort treatment against bacteria. The aim of this research was to determine the occurrence of carbapenem resistant E.coli carrying blaNDM and blaKPC from commercial poultry farms environment in Aba. Three commercial poultry farms which had not less of 5000 birds and not less than 3 years were randomly selected from major areas in Aba (Aba north, Aba south and Osisioma) for the study. 50 samples each from the soil, drinking water and droppings from the three different farms were taken using new sterile containers and transported to the laboratory for further analyses. A tenfold serial dilution of the three samples were done and plated out on freshly prepared sterile Eosin methylene blue (EMB) agar, MacConkey agar, at 44oC and 37oC for 24hours. Purification of isolates was done by subculturing colonies with the green metallic sheen on to fresh EMB agar plates. These colonies were then tested against Imipenem and meropenem and the resistance recorded. The resistance isolates we screened for Cabarpenemase ( KPC and NDM) using molecular detection techniques. Result showed that, Green metallic sheen had an occurrence of 100% on the soil, droppings 96.7% and drinking water 84%. Highest resistant E.coli were isolated from the soil samples and the occurrence of the resistance genes showed that in farm A and C, blaNDM was the higher than blaKPC with a difference of p ? 0.0021 and p?0.0565 among the samples, while in farm B blaKPC was higher with P?0.0051.This study has shown a high level of resistance genes(blaKPC and blaNDM) from these commercial poultry farms and hence the need for public health interventions.
Citations
IRE Journals:
Amarachukwu Ihuoma Nworgu, Jessica Ezinne , Ekeleme "Occurrence of Carbapenem – Resistant Escherichia Coli Carrying Bla NDM and Bla Kpc Genes from Commericlal Poultry Farm Environments in Aba Abia State." Iconic Research And Engineering Journals Volume 9 Issue 7 2026 Page 635-640 https://doi.org/10.64388/IREV9I7-1713271
IEEE:
Amarachukwu Ihuoma Nworgu, Jessica Ezinne , Ekeleme
"Occurrence of Carbapenem – Resistant Escherichia Coli Carrying Bla NDM and Bla Kpc Genes from Commericlal Poultry Farm Environments in Aba Abia State." Iconic Research And Engineering Journals, 9(7) https://doi.org/10.64388/IREV9I7-1713271
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