Occurrence of Carbapenem ? Resistant Escherichia Coli Carrying Bla NDM and Bla Kpc Genes from Commericlal Poultry Farm Environments in Aba Abia State.

References

[1] Adelowo OO, Fagade O.E,& Agersø Y (2014). Antibiotic resistance and resistance genes in Escherichia coli from poultry farms, southwest Nigeria. J Infect Dev Ctries 8:1103–1112. 10.3855/jidc.4222

[2] Aworh, M.K, Kwaga, J.K.P, Hendriksen, R.S, Okolocha, E.C, Thakur, S.(2021). Genetic Relatedness of Multidrug Resistant Escherichia Coli Isolated from Humans, Chickens and Poultry Environments. Antimicrob. Resist. Infect. Control, 10:58.

[3] Bailey J.K, Pinyon J.L, Anantham S,& Hall R.M, (2010). Commensal Escherichia coli of healthy humans: a reservoir for antibiotic- resistance determinants. J Med Microbiol. 59:1331–1339.

[4] Chishimba, K.; Hang’Ombe, B.M.; Muzandu, K.; Mshana, S.E.; Matee, M.I.; Nakajima, C.; Suzuki, Y. (2016).Detection of Extended- Spectrum Beta -Lactamase-Producing Escherichia Coli in Market-Ready Chickens in Zambia. Int. J. Microbiol, 5275724

[5] Gómez-Gómez C, Blanco-Picazo P,& Brown- Jaque M, (2019). Infectious phage particles packaging antibiotic resistance genes found in meat products and chicken feces. Sci Rep 9:13281.

[6] Hartmann A, Amoureux L.& Locatelli A, (2012). Occurrence of CTX-M producing Escherichia coli in soils, cattle, and farm environment in France (Burgundy region) Front Microbiol. 3:83.

[7] Karmali M.A, Gannon V,& Sargeant J.M.( 2010) Verocytotoxin-producing Escherichia coli (VTEC) Vet Microbiol.140:360–370. 10.1016/j.vetmic.2009.04.011.

[8] Nguyen M.C, Woerther P.L, Bouvet M, Andremont A, Leclercq R& Canu A. (2009). Escherichia coli as reservoir for macrolide resistance genes. Emerg Infect Dis. 15:1648.

[9] Nordmann P, Poirel L, Carrer A, Toleman MA and Walsh TR. (2011). How to detect NDM-1 producers. J. Clin. Microbiol. 49(2): 718-721

[10] Radzikowski J.L, Vedelaar Silke, Siegel David, Ortega ÁD, Schmidt Alexander & Heinemann M.(2016). Bacterial persistence is an active stress response to metabolic flux limitation. Mol Syst Biol. 12(9):882.

[11] Smith P,& Christofilogiannis, P. (2007). Application of normalised resistance interpretation to the detection of multiple low- level resistance in strains of Vibrio anguillarum obtained from Greek fish farms. Aquaculture. 272(1-4):223-230

[12] Soufi L, Sáenz Y, & Vinué L, (2011). Escherichia coli of poultry food origin as reservoir of sulphonamide resistance genes and integrons. International J Food Microbiol. 144:497–502. 10.1016/j.ijfoodmicro.2010.11.008.

[13] Wistrand-Yuen Erik, Knopp Michael, Hjort Karin, Koskiniemi Sanna, Berg O. G& Andersson DI. (2018). Evolution of high-level resistance during low-level antibiotic exposure. Nat Commun. 9(1):1599.

@article{1713271,
      author = {Amarachukwu Ihuoma Nworgu, Jessica Ezinne , Ekeleme},
      title = {Occurrence of Carbapenem ? Resistant Escherichia Coli Carrying Bla NDM and Bla Kpc Genes from Commericlal Poultry Farm Environments in Aba Abia State.},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {9},
      number = {7},
      pages = {635-640},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1713271.pdf},
      abstract = {Carbapenem antibiotics are the referred to as the last resort treatment against bacteria. The aim of this research was to determine the occurrence of carbapenem resistant E.coli carrying blaNDM and blaKPC from commercial poultry farms  environment in Aba. Three commercial poultry farms which had not less of 5000 birds and not less than 3 years were randomly selected from major areas in Aba (Aba north, Aba south and Osisioma) for the study. 50 samples each from the soil, drinking water and droppings from the three different farms were taken using new sterile containers and transported to the laboratory for further analyses.  A tenfold serial dilution of the three samples were done and plated out on freshly prepared sterile Eosin methylene blue (EMB) agar, MacConkey agar, at 44oC and 37oC for 24hours. Purification of isolates was done by subculturing colonies with the green metallic sheen on to fresh EMB agar plates. These colonies were then tested against Imipenem and meropenem and the resistance recorded. The resistance isolates we screened for  Cabarpenemase ( KPC and NDM) using molecular detection techniques. Result showed that, Green metallic sheen had an occurrence of 100% on the soil, droppings 96.7% and drinking water  84%. Highest resistant E.coli were isolated from the soil samples and the occurrence of the resistance genes showed that in farm A and C, blaNDM was the higher than blaKPC with a difference of  p ? 0.0021 and p?0.0565 among the samples, while in farm B  blaKPC was higher with  P?0.0051.This study has shown a high level of resistance genes(blaKPC and blaNDM) from these commercial poultry farms and hence the need for public health interventions.},
      month = {January},
      doi = {https://doi.org/10.64388/IREV9I7-1713271}
  }