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From Pod to Product: A Comparative Evaluation of a Biosurfactant-Based Liquid Detergent Formulated from Cola Hispida Agro-Waste
Subject area: Science,Engineering and Technology · Area of research: Biochemistry
DOI: https://doi.org/10.64388/IREV9I7-1713543
Abstract
The transition from laboratory biosurfactant production to functional consumer products remains a critical hurdle for sustainable biotechnology. This study completes a circular bioeconomy model by formulating and evaluating a liquid detergent using glycolipid biosurfactants produced from the fermentation of Cola hispida pod agro-waste. Biosurfactants from individual cultures of a Bacillus sp. and a Pseudomonas sp., and from their synergistic consortium, were integrated as the primary active ingredient into a green detergent base. Performance was assessed through foaming ability and stability. The consortium-derived biosurfactant detergent demonstrated superior foaming performance, producing high, persistent foam (rated +++), outperforming detergents from individual cultures (rated ++). A comparative analysis with existing literature on biosurfactant-based cleaners positions our formulation's performance within the context of established systems, while highlighting its unique origin from underutilized agro-waste. This work successfully closes the valorization chain, providing a tangible proof-of-concept for converting agricultural residue into a market-relevant cleaning product. It underscores a viable model for reducing reliance on petrochemical surfactants while addressing waste management challenges through circular production.
Keywords
Biosurfactant Application, Green Detergent Formulation, Foaming Capacity, Circular Bioeconomy, Agro-Waste Valorization, Cola Hispida, Comparative Performance.
References
[1] S. O. Badmus et al., “Environmental risks and toxicity of surfactants,” Environ. Sci. Pollut. Res., vol. 28, no. 44, pp. 62085–62104, 2021.
[2] E. Jurado et al., “Acute toxicity and metabolite‐ecotoxicity during biodegradation of non‐ionic surfactants,” Water Sci. Technol., vol. 55, no. 5, pp. 131–138, 2007.
[3] R. D. Ashby et al., “Synthesis of PHA blends by mixed culture fermentation of glycerol,” Biomacromolecules, vol. 6, no. 4, pp. 2106–2112, 2005.
[4] I. M. Banat et al., “Microbial biosurfactants production, applications and future potential,” Appl. Microbiol. Biotechnol., vol. 87, no. 2, pp. 427–444, 2010.
[5] I. Mnif and D. Ghribi, “Microbial derived surface active agents,” Microb. Cell Fact., vol. 14, p. 168, 2015.
[6] R. Marchant and I. M. Banat, “Microbial biosurfactants: challenges and opportunities,” Trends Biotechnol., vol. 30, no. 11, pp. 558–565, 2012.
[7] K. C. Ali et al. Volarazation of Agro-waste: Proximate analysis and potential of cola hipsida pod as a novel substrate for microbial biosurfactant production. Open Access Research Journal of Science and Technology, 2025, 15(02), 201-207. https//doi.org/10.53022/oarjst.2025.15.2.0149
[8] K. C. Ali et al. Isolation of native biosurfactant producers and their sustainable cultivation in a novel cola hipsida pod-based medium, International journal of Biological and Pharmaceutical Sciences Archieve, 2025, 10(02), 196-202. htpps//doi.org/10.53771/ijbpsa.2025.10.2.0091
[9] K. C. Ali, T. J. Oluwadepo, C. B. Okeke, E. C. Aham, C. C. Uzoefuna, A. O. Olusegun and H. A. Adewuyi. Functional superiority and robust stability of biosurfactants from a bacillus-pseudomonas consortium cultivated on Cola hipsida pod Agro-wste. Magna Scientia Advanced Researched and Reviews, 2025, 15(02), 298-305. https//doi.org/10.30574/msarr.2025.15.2.0167
[10] K. C. Ali et al., “Volarazation of Agro-waste: Proximate analysis and potential of cola hipsida pod as a novel substrate for microbial biosurfactant production” Open Access Research Journal of Science and Technology, 2025, 15(02), 201-207. https//doi.org/10.53022/oarjst.2025.15.2.0149
[11] M. J. Rosen and J. T. Kunjappu, Surfactants and Interfacial Phenomena, 4th ed. Hoboken: Wiley, 2012.
[12] C. A. Miller and P. Neogi, Interfacial Phenomena, 2nd ed. Boca Raton: CRC Press, 2007.
[13] A. M. Shete et al., “Mapping of patents on bioemulsifier and biosurfactant,” J. Sci. Ind. Res., vol. 65, no. 2, pp. 91–115, 2006.
[14] A. K. Mukherjee, “Application of Bacillus lipopeptides in laundry detergents,” Lett. Appl. Microbiol., vol. 45, no. 3, pp. 330–335, 2007.
[15] O. S. Azeez and G. S. Abegunde, “Development of eco‐friendly liquid detergent from plant‐based surfactants,” Niger. J. Technol., vol. 35, no. 1, pp. 60–65, 2016.
[16] British Standards Institution, BS EN 12728:2000. Determination of foaming power. London: BSI, 2000.
[17] I. E. Kłosowska‐Chomiczewska et al., “Rhamnolipid CMC prediction,” J. Colloid Interface Sci., vol. 503, pp. 10–17, 2017.
[18] D. Exerowa and P. M. Kruglyakov, Foam and Foam Films. Amsterdam: Elsevier, 1998.
[19] C. B. B. Farias et al., “Production of green surfactants: Market prospects,” Electron. J. Biotechnol., vol. 51, pp. 28–39, 2021.
How to cite this paper
@article{1713543,
author = {Kingsley Chijioke Ali, Arinzechukwu Emmanuel Onovo, Adeniyi Oyeyemi Olusegun, Chima Casmir Uzoefuna; Timileyin Joshua Oluwadepo, Emmanuel Chigozie Aham; Hassan Abdulsalam Adewuyi},
title = {From Pod to Product: A Comparative Evaluation of a Biosurfactant-Based Liquid Detergent Formulated from Cola Hispida Agro-Waste},
journal = {Iconic Research And Engineering Journals},
year = {2026},
volume = {9},
number = {7},
pages = {1089-1094},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1713543.pdf},
abstract = {The transition from laboratory biosurfactant production to functional consumer products remains a critical hurdle for sustainable biotechnology. This study completes a circular bioeconomy model by formulating and evaluating a liquid detergent using glycolipid biosurfactants produced from the fermentation of Cola hispida pod agro-waste. Biosurfactants from individual cultures of a Bacillus sp. and a Pseudomonas sp., and from their synergistic consortium, were integrated as the primary active ingredient into a green detergent base. Performance was assessed through foaming ability and stability. The consortium-derived biosurfactant detergent demonstrated superior foaming performance, producing high, persistent foam (rated +++), outperforming detergents from individual cultures (rated ++). A comparative analysis with existing literature on biosurfactant-based cleaners positions our formulation's performance within the context of established systems, while highlighting its unique origin from underutilized agro-waste. This work successfully closes the valorization chain, providing a tangible proof-of-concept for converting agricultural residue into a market-relevant cleaning product. It underscores a viable model for reducing reliance on petrochemical surfactants while addressing waste management challenges through circular production.},
keywords = {Biosurfactant Application, Green Detergent Formulation, Foaming Capacity, Circular Bioeconomy, Agro-Waste Valorization, Cola Hispida, Comparative Performance.},
month = {January},
doi = {https://doi.org/10.64388/IREV9I7-1713543}
}