Home / Current Issue / Paper 1713736
Amended Treatment of Pb-Polluted Soil Using Animal Dung Composites and Micrococcus sp.: Evaluating their Effects on Maize seedlings Growth
Subject area: Agriculture and Veterinary Sciences · Area of research: Bioremediation
DOI: https://doi.org/10.64388/IREV9I7-1713736
Abstract
Here, amended bioremediation of Pb-polluted soil with poultry waste, cow dung and their consortium, in addition to Micrococcus sp., used as plant growth-promoting rhizobacteria (PGPR), was carried out, and their effects on maize seedlings growth evaluated. After 25 days period of treatment of Pb-polluted soil and germination of corn seeds planted on them, growth parameters including height of shoot, and number of leaves were recorded on day 4, weeks 2, 4 and 7. Results revealed an increase in the pH, organic carbon content and soil organic matter content of the samples soil after treatment. Treatment with cow dung alone produced significantly highest alkalinity of 12.18% at 33.75 mg/kg, 14.18% at 44.02 mg/kg, and 10.05% at 52.55 mg/kg Pb pollution. The N and P contents of all treated soil samples were significantly higher than those of control samples. There was increment in the dehydrogenase activity in all treated Pb-polluted soil samples. The chlorophyll contents of seedlings grown on treated samples were more than those of seedlings on control samples. The seedlings on soil treated with cow dung only, produced the highest total chlorophyll contents of 1.06?0.07 mg/g at 32.75 mg/kg, 0.71?0.02 mg/g at 42.02 mg/kg and 0.68?0.09 mg/g total chlorophyll at 52.55 mg/kg levels of Pb pollution. The range of Pb bioaccumulation in maize leaf was 1.26?0.07 mg/kg to 3.8?0.05 mg/kg; 0.78?0.04 mg/kg to 1.84?0.1 mg/kg in the stem and 4.34?0.07 mg/kg to 8.7?0.15 mg/kg in root samples. These results prove that the amendments used in the study enhanced the seedlings growth.
Keywords
Micrococcus Sp., Bioremediation, Toxic Metals, Pollution, Plant Growth
References
[1] Adak, T., Singha, A., Kumar, K., Shukla, S.K., Singh, A. and Singh, V.K. (2014). Soil organic carbon, dehydrogenase activity, nutrient availability and leaf nutrient content as affected by organic and inorganic source of nutrient in mango orchard soil. Journal of Soil Science and Plant Nutrition. 2: 394-406.
[2] Adebiyi, K.A. and Salami, A.O. (2023). Effect of manure and Glomus hoi on heavy metals and soil properties of spent engine oil contaminated soil. International Journal of Plant & Soil Science. 35(19): 487-501. https://doi.org/10.9734/IJPSS/2023/v35i193575
[3] Alves, A.R.A., Yin, Q., Oliveirad, R.S., Silva, E.F. and Novo, L.A.B. (2022). Plant growth-promoting bacteria in phytoremediation of metal-polluted soils: Current knowledge and future directions. The Science of the Total Environment. 838(156435): 1-10. http://dx.doi.org/10.1016/j.scitotenv.2022.156435
[4] Angelova, V.R., Akova, V.I., Artinova, N.S. & Ivanov, K.I. (2013). The effect of organic amendments on soil chemical characteristics. Bulgarian Journal of Agricultural Science, 19: 958-971.
[5] Anuforo, H.U., Akujobi, C.O., Ezeji, E.U., Okehi, C.C. (2019). Impact of vehicular traffic on concentrations of selected heavy metals in cassava tubers harvested from roadside in Owerri, Nigeria. Journal of Faculty of Food Engineering, Ştefan cel Mare University of Suceava, Romania. 18(4): 272-278.
[6] Anuforo, H.U., Ogbulie, T.E., Elumezie, A.O. and Nwachukwu, A.A. (2020a). Impact of heavy metals on safety of cattle meat sold in Owerri Metropolis, Imo State, Nigeria. Environmental Engineering and Management Journal, 19(11): 2013-2019.
[7] Anuforo, H.U., Akujobi, C.O., Umeh, P.K., Ejimadu, P.I. (2020b). Pattern of distribution and concentration of selected heavy metals in farmlands near roadsides in Owerri, Nigeria. Analele Universităţii din Oradea, Fascicula Biologie, XXVII(1), 32-38.
[8] Ashraf, S., Ahmad, S.R., Ali, Q., Ashraf, S., Majid, M., and Zahir, Z.A. (2022). Acidified cow dung-assisted phytoextraction of heavy metals by ryegrass from contaminated soil as an eco-efficient technique. Sustainability. 14(15879): 1-17. https://doi.org/10.3390/su142315879
[9] Irfan, M. Mudassir, M., Khan, M.J., Dawar, K.M., Muhammad, D., Mian, I.A., Ali, W., Fahad, S., and Dewil. R. (2021). Heavy metals immobilization and improvement in maize (Zea mays L.) growth amended with biochar and compost. Scientific Reports. 11(18416): 1-9.
[10] Islam, F., Yasmeen, T., Ali, Q., Ali, S., Arif, M.S., Hussain, S. (2014). Influence of Pseudomonas aeruginosa as PGPR on oxidative stress tolerance in wheat under Zn stress. Ecotoxicology and Environmental Safety. 104: 285-293.
[11] Jawale, S.A., Patil, V.D., Jawale, S.A. and Satpute, U. (2017). Effect of soil fertility levels on chlorophyll content of maize crop. Journal of Pharmacognosy and Phytochemistry. 6(6): 95-97.
[12] Korie, M.C., Osuala, F.O.U., Ogbulie, J.N., Okereke, J.N., Anyadoh-Nwadike, S.O., Ibe, C.C., and Nwachukwu, M.E. (2024a). Evaluation of effects of animal dung composites and Bacillus sp in amended bioremediation of Zn-polluted soil on maize seedlings growth. World Journal of Advanced Research and Reviews, 22(01): 991–1004. https://doi.org/10.30574/wjarr.2024.22.1.1152
[13] Korie, M.C., Osuala, F.O.U., Ogbulie, J.N., Okereke, J.N., Anyadoh-Nwadike, S.O., Ibe, C.C. and Nwachukwu, M.E. (2024b). Assessment of effects of amended bioremediation of toxic metal-polluted soil using organic composites and Bacillus sp on plant growth parameters. Biotechnology Journal International. 28(3): 21-38. https://doi.org/10.9734/BJI/2024/v28i3721
[14] Lu, Y., Yao, H., Shan, D., Jiang, Y., Zhang, S. and Yang, J. (2015). Heavy metal residues in soil and accumulation in maize at long-term wastewater irrigation area in Tongliao, China. Journal of Chemistry. 628280: 1-9.
[15] Pinter, I.F., Salomon, M.V., Berli, F., Bottini, R., Piccoli, P. (2017). Characterization of the As (III) tolerance conferred by plant growth promoting rhizobacteria to in vitro-grown grapevine. Appl. Soil Ecol., 109: 60–68. https://doi.org/10.1016/j.apsoil.2016.10.003.
[16] Qin, H., Wang, Z., Sha, W., Song, S., Qin, F. and Zhang, W. (2024). Role of plant-growth-promoting rhizobacteria in plant machinery for soil heavy metal detoxification. Microorganisms. 12(04): 700. https://doi.org/10.3390/microorganisms12040700
[17] Riseh, R.S., Vazvani, M.G., Hajabdollahi, N. and Thakur, V.K. (2023). Bioremediation of heavy metals by rhizobacteria. Applied Biochemistry and Biotechnology, 195: 4689-4711. https://doi.org/10.1007/s12010-022-04177-z
[18] Romdhane, L., Panozzo, A., Radhouane, L., Dal Cortivo, C., Barion, G. and Vamerali, T. (2021). Root characteristics and metal uptake of maize (Zea mays L.) under extreme soil contamination. Agronomy. 11: 178.
[19] Santhini, K., Myla, J., Sajani, S. and Usharani, G. (2009). Screening of Micrococcus sp from oil contaminated soil with reference to bioremediation. Botany Research International. 2(4): 248-252.
[20] Sarkar, S., Mondal, M., Ghosh, P., Saha, M. and Chatterjee, S. (2020). Quantification of total protein content from some traditionally used edible plant leaves: A comparative study. Journal of Medicinal Plants Studies. 8(4): 166-170. https://doi.org/10.22271/plants.2020.v8.i4c.1164
[21] Souza, S.R., Oliveira, B.F., Pimentel, J.C., Ramos, S.A., Araújo, J.M., Napoleão, T.H., Paiva, P.M.G., Correia, M.T., and Coelho, L.C.B. (2021). A simple electrochemical system to evaluate interactions between Bauhinia monandra leaf lectin (BmoLL) and Cratylia mollis seed lectin (cramoll) immobilized in nafion nanopores with Pseudomonas aeruginosa endophytic strain. Recent Research Advances in Biology. 9: 1-17.
[22] Taalab, A.S. and Shahin, R.R. (2018). Effect of fertilizer application on the uptake of heavy metals by corn plant grown in different soils. Middle East Journal of Agriculture. 07(02): 505-511.
[23] Wojewódzki, P., Lemanowicz, J., Debska, B. and Haddad, S.A. (2022). Soil enzyme activity response under the amendment of different types of biochar. Agronomy. 12: 569. https://doi.org/10.3390/agronomy12030569
How to cite this paper
@article{1713736,
author = {Korie, Maximus Chibuoyi, Nwabueze, Ekene Uzoma, Echeta, MaryRose Ogechi; Nzeagwu, Maximus Obinna, Onyema, Clifford T.; Ochia, Angela MMA., Unegbu Valentine Nnachetam; Itubochi, Calista Odinachi},
title = {Amended Treatment of Pb-Polluted Soil Using Animal Dung Composites and Micrococcus sp.: Evaluating their Effects on Maize seedlings Growth},
journal = {Iconic Research And Engineering Journals},
year = {2026},
volume = {9},
number = {7},
pages = {1904-1916},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1713736.pdf},
abstract = {Here, amended bioremediation of Pb-polluted soil with poultry waste, cow dung and their consortium, in addition to Micrococcus sp., used as plant growth-promoting rhizobacteria (PGPR), was carried out, and their effects on maize seedlings growth evaluated. After 25 days period of treatment of Pb-polluted soil and germination of corn seeds planted on them, growth parameters including height of shoot, and number of leaves were recorded on day 4, weeks 2, 4 and 7. Results revealed an increase in the pH, organic carbon content and soil organic matter content of the samples soil after treatment. Treatment with cow dung alone produced significantly highest alkalinity of 12.18% at 33.75 mg/kg, 14.18% at 44.02 mg/kg, and 10.05% at 52.55 mg/kg Pb pollution. The N and P contents of all treated soil samples were significantly higher than those of control samples. There was increment in the dehydrogenase activity in all treated Pb-polluted soil samples. The chlorophyll contents of seedlings grown on treated samples were more than those of seedlings on control samples. The seedlings on soil treated with cow dung only, produced the highest total chlorophyll contents of 1.06?0.07 mg/g at 32.75 mg/kg, 0.71?0.02 mg/g at 42.02 mg/kg and 0.68?0.09 mg/g total chlorophyll at 52.55 mg/kg levels of Pb pollution. The range of Pb bioaccumulation in maize leaf was 1.26?0.07 mg/kg to 3.8?0.05 mg/kg; 0.78?0.04 mg/kg to 1.84?0.1 mg/kg in the stem and 4.34?0.07 mg/kg to 8.7?0.15 mg/kg in root samples. These results prove that the amendments used in the study enhanced the seedlings growth.},
keywords = {Micrococcus Sp., Bioremediation, Toxic Metals, Pollution, Plant Growth},
month = {January},
doi = {https://doi.org/10.64388/IREV9I7-1713736}
}