Genotoxicity of Glyphosate (Pesticide) Using The Allium Cepa L. Assay.
  • Author(s): James, Onisodumeya Elemchukwu; Adetutu Olubunmi Obulor
  • Paper ID: 1714745
  • Page: 339-348
  • Published Date: 10-03-2026
  • Published In: Iconic Research And Engineering Journals
  • Publisher: IRE Journals
  • e-ISSN: 2456-8880
  • Volume/Issue: Volume 9 Issue 9 March-2026
Abstract

The use of pesticides because of their importance cannot be overemphasized. Although there are warnings of the health implications posed by pesticides to lives due to their uncontrolled use. It is expedient that, glyphosate (a commonly used pesticide in Nigeria) be investigated for its toxic effects. This study investigated the effect of glyphosate using the Allium cepa assay. The roots of onion were exposed for 24 hours to varying treatment concentrations (0g/L, 0.75g/L, 1.5g/L, 3.0g/L, 6.0g/L, and 8g/L of glyphosate). Microscopic end points of different chromosomal aberrations were evaluated and it was observed that glyphosate induced genotoxic effects such as stickiness, micro nuclei, lagging chromosomes, C - mitosis, and nuclear lesion. Macroscopic parameters such as root length and number of roots were also evaluated. Obtained data were statistically analyzed at 0.05 (5%) level of significance using analysis of variance and the Student’s t-test. Root growth was retarded with increase in concentrations of glyphosate. Statistically, significant (P<0.05) differences were established between root lengths exposed to glyphosate when compared with the control treatment. However, number of roots showed no significant statistical difference when compared with the control treatment. The findings showed that glyphosate can pose adverse effects on lives and the environment at large. It is recommended that increased awareness and enlightenment of the public on the dangers associated with the use of pesticides and the need for adherence to safety measures be encouraged by the regulatory agencies saddled with responsibilities to do so.

Keywords

Genotoxicity, Pesticide, Allium cepa Assay, Glyphosate.

Citations

IRE Journals:
James, Onisodumeya Elemchukwu, Adetutu Olubunmi Obulor "Genotoxicity of Glyphosate (Pesticide) Using The Allium Cepa L. Assay." Iconic Research And Engineering Journals Volume 9 Issue 9 2026 Page 339-348 https://doi.org/10.64388/IREV9I9-1714745

IEEE:
James, Onisodumeya Elemchukwu, Adetutu Olubunmi Obulor "Genotoxicity of Glyphosate (Pesticide) Using The Allium Cepa L. Assay." Iconic Research And Engineering Journals, 9(9) https://doi.org/10.64388/IREV9I9-1714745