Invitro Antioxidant, Antimicrobial and Bioassay Guided-Fractionation of Methanol Extract of Pericopsis Laxifora Leaf

References

[1] S. O. Anifowose, A. M. Salih, M. K. Oladejo, A. Rady, M. S. Al Mosallam, H. A. Aljohi, M. I. Almansour, S. H. Alkahtani, I. O. Alanazi, & B. A. Al-Dahmash, "Bioactivity-guided fractionation and mechanistic insights into Aristolochia ringens root extract-induced G₁ phase arrest and mitochondria-mediated apoptosis in human colon adenocarcinoma cells," Pharmaceutics, vol. 18, no. 9, pp. 1250, 2025. https://doi.org/10.3390/ph18091250.

[2] M. A. Baldé, M. S. Traoré, M. S. T. Diallo, A. Matheeussen, A. Camara, P. Cos, L. Maes, A. O. Baldé, M. K. Camara, A. M. Balde, E. Tuenter, & K. Foubert, "Isolation and characterization of antiplasmodial and antimicrobial compounds from Tetracera alnifolia using a bioassay-guided approach," RSC Adv., vol. 16, pp. 10635–10647, 2026. https://doi.org/10.1039/D5RA09498D.

[3] P. C. Chaudhary, P. Janmeda, A. O. Docea, B. Yeskaliyeva, A. F. Abdull Razis, B. Modu, D. Calina, & J. Sharifi-Rad, "Oxidative stress, free radicals and antioxidants: Potential crosstalk in the pathophysiology of human diseases," Front. Chem., vol. 11, pp. 1158198, 2023. https://doi.org/10.3389/fchem.2023.1158198.

[4] L. De Rossi, G. Rocchetti, L. Lucini, & A. Rebecchi, "Antimicrobial potential of polyphenols: Mechanisms of action and microbial responses—A narrative review," Antioxidants, vol. 14, no. 2, pp. 200, 2025. https://doi.org/10.3390/antiox14020200.

[5] D. N. Doukourou, R. Kamou, B. Bamba, & K. Ouattara, "Phytochemical screening, mineral analysis and evaluation of the diuretic effect of aqueous extract of Pericopsis laxiflora from male Sprague Dawley rats," J. Drug Deliv. Ther., 2025. https://jddtonline.info/index.php/jddt/article/view/4953.

[6] GBD 2021 Antimicrobial Resistance Collaborators, "Global burden of bacterial antimicrobial resistance 1990–2021: A systematic analysis with forecasts to 2050," Lancet, vol. 404, no. 10459, pp. 1199–1226, 2024. https://doi.org/10.1016/S0140-6736(24)01867-1.

[7] K. A. Jeanne, S. Diarra, O. C. Okou, K. D. Tano, A. F. Koffi, K. Rasmane Na Ahou, L. E. Akissi Zachée, & W. Yavo, "In vivo evaluation of the acute toxicity and anti-inflammatory effects of the methanolic extract and acetatic fraction of Pericopsis laxiflora bark in the management of malaria," J. Adv. Biol. Biotechnol., vol. 29, no. 1, pp. 19–30, 2026. https://doi.org/10.9734/jabb/2026/v29i13503.

[8] M. D. Jović, S. Agatonovic-Kustrin, P. M. Ristivojević, J. Đ. Trifković, & D. W. Morton, "Bioassay-guided assessment of antioxidative, anti-inflammatory and antimicrobial activities of extracts from medicinal plants via high-performance thin-layer chromatography," Molecules, vol. 28, no. 21, pp. 7346, 2023. https://doi.org/10.3390/molecules28217346.

[9] H. K. K. Krishnamurthy, M. Pereira, I. Rajavelu, V. Jayaraman, K. Krishna, T. Wang, K. Bei, & J. J. Rajasekaran, "Oxidative stress: Fundamentals and advances in quantification techniques," Front. Chem., vol. 12, pp. 1470458, 2024. https://doi.org/10.3389/fchem.2024.1470458.

[10] J. Mani, J. Johnson, H. Hosking, B. E. Hoyos, K. B. Walsh, P. Neilsen, & M. Naiker, "Bioassay guided fractionation protocol for determining novel active compounds in selected Australian flora," Plants, vol. 11, no. 21, pp. 2886, 2022. https://doi.org/10.3390/plants11212886.

[11] C. J. L. Murray, K. S. Ikuta, F. Sharara, L. Swetschinski, G. R. Aguilar, A. Gray, C. Han, C. Bisignano, P. Rao, E. Wool, S. C. Johnson, A. J. Browne, M. G. Chipeta, F. Fell, S. Hackett, G. Haines-Woodhouse, B. H. Kashef Hamadani, E. A. P. Kumaran, B. McManigal, … M. Naghavi, "Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis," Lancet, vol. 399, no. 10325, pp. 629–655, 2022. https://doi.org/10.1016/S0140-6736(21)02724-0.

[12] G. K. Oloyede & L. O. Bello, "Chemical composition, antioxidant and antimicrobial activities of volatile oils of Periscopsis laxiflora leaf and stem," Bull. Chem. Soc. Ethiop., vol. 36, no. 3, pp. 717–725, 2022. https://doi.org/10.4314/bcse.v36i3.20.

[13] A. Ouattara, D. S.-T. Akre, & T. Kamagate, "Phenolic compound contents, anti-inflammatory activities and effect of hydroethanolic extract of Pericopsis laxiflora (Benth.) roots on prostate-specific antigen content in male Wistar rats induced with benign prostatic hyperplasia," J. Appl. Life Sci. Int., vol. 28, no. 6, pp. 101–111, 2025. https://doi.org/10.9734/jalsi/2025/v28i6737.

[14] A. Ouattara, M. Koné, K. J. Golly, K. Ouattara, & A. Coulibaly, "Acute and sub-acute toxicity study of aqueous extract of Pericopsis (Afrormosia) laxiflora (Benth.) on white albino rats," J. Pharmacogn. Phytochem., vol. 9, no. 6, pp. 22–25, 2020. https://doi.org/10.22271/phyto.2020.v9.i6a.12905.

[15] Y. Qin, C. Qian, W. Li, Q. Wang, Q. Sheng, Z. Chen, W. Zhang, W. Li, G. Ge, Z. Yan, & D. Geng, "Oxidative stress: Molecular mechanisms, diseases, and therapeutic targets," MedComm (2020), vol. 7, no. 2, e70600, 2026. https://doi.org/10.1002/mco2.70600.

[16] F. Sarfo-Antwi, C. Larbie, B. O. Emikpe, & R. Appiah–Opong, "Ethnobotany, phytochemistry and pharmacology of Pericopsis laxiflora (Baker) Meeuwen (Leguminosae) – A review," Int. J. Biochem. Res. Rev., vol. 30, no. 2, pp. 1–7, 2021. https://doi.org/10.9734/ijbcrr/2021/v30i230248.

[17] F. Sarfo-Antwi, C. Larbie, B. O. Emikpe, & R. Appiah–Opong, "Hydroethanolic extract and fractions of Pericopsis laxiflora stem bark protect against liver injury in rat," Sci. Afr., 2024. https://doi.org/10.1016/j.sciaf.2024.e02186.

[18] R. O. Tijani, S. O. Lawal, J. O. Oyekan, O. K. Koleoso, S. S. Onasanya, & A. A. Fasasi, "Anti-diabetic effect of methanol extract of Aristolochia ringens leaf," Biochem. Anal. Biochem., vol. 10, pp. 397, 2021. https://doi.org/10.35248/2161-1009.21.10.397.

[19] World Health Organization, "Antimicrobial resistance (Fact sheet)," November 21, 2023. https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance.

[20] World Health Organization, "WHO bacterial priority pathogens list, 2024: Bacterial pathogens of public health importance to guide research, development and strategies to prevent and control antimicrobial resistance," 2024. https://www.who.int/publications/i/item/9789240093461.

[21] N. Zouine, N. El Ghachtouli, S. El Abed, & S. Ibnsouda Koraichi, "A comprehensive review on medicinal plant extracts as antibacterial agents: Factors, mechanism insights and future prospects," Sci. Afr., vol. 26, e02395, 2024. https://doi.org/10.1016/j.sciaf.2024.e02395

@article{1715092,
      author = {Aminu S. O., Lawal Semiu O, Adekolurejo Ezekiel, Abidoye R. A.},
      title = {Invitro Antioxidant, Antimicrobial and Bioassay Guided-Fractionation of Methanol Extract of Pericopsis Laxifora Leaf},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {9},
      number = {9},
      pages = {1709-1720},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1715092.pdf},
      abstract = {This study investigated the in vitro antioxidant and antimicrobial activities of the Methanol extract of Pericopsis laxiflora leaf (MLPL) and its bioassay-guided fractions, aiming to identify the most active constituents. Phytochemical screening of MLPL and its fractions revealed the presence of flavonoids, anthraquinones, tannins, saponins, and alkaloids, with notable variations across fractions. Antioxidant activity was evaluated using DPPH radical scavenging, lipid peroxidation (MDA), nitric oxide, superoxide radical scavenging, and reducing power assays. The ethyl acetate (EAFPL) and butanol (BFPL) fractions consistently demonstrated superior antioxidant activity, with EAFPL showing the highest potency across all models, correlating with its high phenolic content. Antimicrobial activity was assessed against bacterial pathogens (Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa) and fungal strains (Candida albicans, Aspergillus spp.). EAFPL again exhibited the strongest inhibition, indicating broad-spectrum antimicrobial potential. The study highlights the relevance of bioassay-guided fractionation in isolating potent phytochemicals responsible for biological activity. Clinically, the antioxidant and antimicrobial properties of EAFPL suggest potential therapeutic applications in oxidative stress-related diseases and microbial infections. Overall, P. laxiflora leaves are a promising source of bioactive compounds which serve as intervention in the management of oxidative stress-linked diseases and microbial infections.},
      keywords = {Antimicrobial, Bio-Guided Fractionation, Medicinal Plants, Oxidative Stress, Phytochemical Profile.},
      month = {March},
      doi = {https://doi.org/10.64388/IREV9I9-1715092}
  }