Chemical Composition and Phytochemical Profiling of Phyllanthus Niruri Aqueous Leaf Extract

References

[1] Bagalkotkar, G., Sagineedu, S. R., Saad, M. S., & Stanslas, J. (2006). Phytochemicals from Phyllanthus niruri Linn. and their pharmacological properties: A review. Journal of Pharmacy and Pharmacology, 58(12), 1559–1570.

[2] Narendra, K., Swathi, J., Sowjanya, K. M., & Satya, A. K. (2012). Phyllanthus niruri: A review on its ethnopharmacological, phytochemical and pharmacological profile. Journal of Pharmacy Research, 5(9), 4681–4691.

[3] Harish, R., & Shivanandappa, T. (2006). Antioxidant activity and hepatoprotective potential of Phyllanthus niruri. Food Chemistry, 95(2), 180–185.

[4] Kaur, N., Kaur, B., & Sirhindi, G. (2017). Phyllanthus niruri: A review on its ethnobotanical, phytochemical and pharmacological profile. Journal of Pharmacognosy and Phytochemistry, 6(4), 468–473.

[5] Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis (3rd ed.). Chapman and Hall, London.

[6] AOAC. (2016). Official Methods of Analysis of the Association of Official Analytical Chemists (20th ed.). Washington DC, USA.

[7] Sofowora, A. (2008). Medicinal Plants and Traditional Medicine in Africa (3rd ed.). Spectrum Books Ltd., Ibadan.

[8] Trease, G. E., & Evans, W. C. (2009). Pharmacognosy (16th ed.). Saunders Elsevier, London.

[9] Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555–559.

[10] Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144–158.

[11] Pearson, D. (1976). The Chemical Analysis of Foods (7th ed.). Churchill Livingstone, Edinburgh.

[12] Obadoni, B. O., & Ochuko, P. O. (2001). Phytochemical studies and comparative efficacy of the crude extracts of some homeostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Sciences, 8(2), 203–208.

[13] Regis Correa da Silva, D. (2024). GC-MS analytical procedures for phytochemical determination. Analytical Chemistry Methods, 14(3), 221–230.

[14] Akinpelu, D. A., & Onakoya, T. M. (2006). Antimicrobial activities of medicinal plants used in folklore remedies in south-western Nigeria. African Journal of Biotechnology, 5(11), 1078–1081.

[15] Olaleye, M. T. (2007). Cytotoxicity and antibacterial activity of methanolic extract of Phyllanthus niruri. Journal of Medicinal Plants Research, 1(3), 26–30.

@article{1718158,
      author = {Achilike, J. J., Ujowundu, C. O., Nwaogu, L. A., Iheme, C. I.; Emeagi, O. H., Njemanze, O. C.; Onyeulor, C. F.},
      title = {Chemical Composition and Phytochemical Profiling of Phyllanthus Niruri Aqueous Leaf Extract},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {9},
      number = {11},
      pages = {3884-3889},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1718158.pdf},
      abstract = {This study investigated the chemical composition, phytochemical constituents, mineral profile, and vitamin composition of Phyllanthus niruri aqueous leaf extract using standard analytical techniques. Gas chromatography–mass spectrometry (GC-MS) analysis showed the presence of several bioactive volatile compounds, including 11-octadecanoic acid methyl ester (33.38%), 9,12-octadecadienoic acid methyl ester (21.45%), hexadecanoic acid methyl ester (14.61%), and 1-octadecene (8.78%). Qualitative phytochemical screening confirmed the presence of flavonoid, alkaloid, phenol, tannin, saponin, and curcumin. Quantitative phytochemical analysis showed alkaloid as the most abundant phytochemical constituent (68.76 ± 0.49 mg/g), phenol (57.70 ± 0.42 mg/g) and flavonoid (30.46 ± 0.08 mg/g) respectively. Mineral analysis indicated that calcium possessed the highest concentration (9.04 ± 0.02 ppm), while sodium had the lowest value (0.47 ± 0.01 ppm). Water-soluble vitamin analysis revealed that vitamin C was the most abundant (49.32 ± 0.00 mg/L), whereas vitamin E exhibited the highest concentration among fat-soluble vitamins (91.74 ± 0.01 mg/L). Proximate analysis showed high protein content (64.71 ± 0.44%), indicating the nutritional significance of the plant. The findings suggest that P. niruri aqueous leaf extract is rich in essential bioactive compounds and nutrients that may contribute to its medicinal efficacy and antioxidant properties. Therefore, P. niruri possesses strong potential for pharmaceutical, nutraceutical, and therapeutic applications.},
      keywords = {Phyllanthus Niruri, Phytochemicals, GC-MS, Minerals, Vitamins, Aqueous Leaf Extract, Antioxidants.},
      month = {May},
      doi = {https://doi.org/10.64388/IREV9I11-1718158}
  }