International Peer-Reviewed JournalOpen AccessISSN 2456-8880
irejournals@gmail.com+91-7433024337

Home / Current Issue / Paper 1718486

1718486 Vol 9 · Issue 11 Download Paper

A Critical Review of MICP and EICP for Sustainable Soil Stabilization Mechanisms, Engineering Performance, Environmental Trade-Offs, and Implementation Challenges

Odunewu Itunu Deborah Ayininuola G. M. Akolade A. S. Adebayo K. J.

Subject area: Science,Engineering and Technology  ·  Area of research: Geotechnical Engineering

DOI: https://doi.org/10.64388/IREV9I11-1718486

Abstract

Traditional cement- and lime-based soil stabilization's environmental constraints have driven the search for sustainable alternatives in geotechnical engineering. Bio-mediated methods like Microbial-Induced Calcite Precipitation (MICP) and Enzyme-Induced Calcite Precipitation (EICP) are gaining attention for their ability to improve soil properties through biologically induced calcium carbonate. This review covers recent advances (2020–2026) on mechanisms, performance, sustainability, and challenges of MICP and EICP for soil stabilization, based on about 120 peer-reviewed studies from major databases. EICP typically shows better penetration in fine soils due to no bacterial size limits, while MICP offers stronger bonding in granular soils from bacterial nucleation. Significant progress has been made in strength, permeability, erosion, and swelling, but issues like non-uniform treatment, ammonia release, brittleness, cost, and durability remain barriers. Tropical lateritic soils are understudied despite their importance in developing countries. Emerging techniques like non-ureolytic methods, fiber systems, nanomaterials, and waste reagents show promise for sustainability and field use. Future work should focus on field validation, durability testing, eco-friendly precipitation, and standard design to move bio-stabilization from research to real-world infrastructure.

Keywords

Microbial-Induced Calcite Precipitation, Enzyme-Induced Calcite Precipitation, Biomineralization, Sustainable Soil Stabilization, Bio-Cementation.

References

[1] Abdullah, S. J., Fattah, M. Y., & Al-Adili, A. S. G. (2026). Sustainability and durability of bio-enzymatic stabilization techniques for collapsible gypseous soils: A review. Jurnal Pensil, 15(1). https://doi.org/10.21009/jpensil.v15i1.62734

[2] Agnesia, N. V. (2026). Modification of SCU-CP method to increase shear strength of sandy soil using chitosan and skim milk. Progress in Engineering Science.

[3] Ahenkorah, I., Rahman, M. M., Karim, M. R., & Beecham, S. (2021). Enzyme induced calcium carbonate precipitation and its engineering application: A systematic review and meta-analysis. Construction and Building Materials. https://doi.org/10.1016/j.conbuildmat.2021.125000

[4] Ali, M. S., & Almurshedi, A. D. (2024). Bio-cementations: A review on enzyme and microbially induced calcite precipitation mechanisms and applications in geotechnical engineering. Journal of Engineering, 30(12), 167. https://doi.org/10.31026/j.eng.2024.12.11

[5] Almajed, A., Abbas, H., Arab, M., Alsabhan, A., Hamid, W., & Al-Salloum, Y. (2020). Enzyme-induced carbonate precipitation (EICP)-based methods for ecofriendly stabilization of different types of natural sands. Journal of Cleaner Production, 274, 122627.

[6] Almajed, A., Lateef, M. A., Moghal, A. A. B., & Lemboye, K. (2021). State-of-the-art review of the applicability and challenges of microbial-induced calcite precipitation (MICP) and enzyme-induced calcite precipitation (EICP) techniques for geotechnical and geoenvironmental applications. Crystals, 11(4), 370. https://doi.org/10.3390/cryst11040370

[7] Almajed, A., Moghal, A. A. B., Nuruddin, M., & Mohammed, S. A. S. (2024). Comparative studies on the strength and swell characteristics of cohesive soils using lime and modified enzyme-induced calcite precipitation technique. Buildings, 14(4), 909. https://doi.org/10.3390/buildings14040909

[8] Alotaibi, E., Arab, M. G., Abdallah, M., Nassif, N., & Omar, M. (2022). Life cycle assessment of biocemented sands using enzyme induced carbonate precipitation (EICP) for soil stabilization applications. Scientific Reports, 12(1), 6032. https://doi.org/10.1038/s41598-022-09723-7

[9] Al-Riahi, S. M. H., Pauzi, N. I. M., Fattah, M. Y., & Abbas, H. A. (2024). Leaching-induced alterations in the geotechnical and microstructural attributes of clayey gypseous soils. Ain Shams Engineering Journal, 15(7), 102865. https://doi.org/10.1016/j.asej.2024.102865

[10] Arab, M. G., Alsodi, R., Almajed, A., Yasuhara, H., & Zeiada, W. (2021). State-of-the-art review of enzyme-induced calcite precipitation (EICP) for ground improvement: Applications and prospects. Geosciences, 11(12), 492. https://doi.org/10.3390/geosciences11120492

[11] Arpajirakul, S., Pungrasmi, W., & Likitlersuang, S. (2021). Efficiency of microbially-induced calcite precipitation in natural clays for ground improvement. Construction and Building Materials, 282, 122722. https://doi.org/10.1016/j.conbuildmat.2021.122722

[12] Baidya, P., Dahal, B. K., Pandit, A., & Joshi, D. R. (2023). Bacteria-induced calcite precipitation for engineering and environmental applications. Advances in Materials Science and Engineering, 2023, 2613209. https://doi.org/10.1155/2023/2613209

[13] Bodner, M., Abdel-Gawwad, H. A., Rackwitz, F., & Stephan, D. (2026). A review of sustainable ground improvement methods and their possible application to organic soils. International Journal of Geosynthetics and Ground Engineering, 12(1), 8. https://doi.org/10.1007/s40891-026-00694-7

[14] Boruah, R. P., Mohanadhas, B., & Jayakesh, K. (2025). Microbially induced calcite precipitation for soil stabilization: A state-of-art review. Geomicrobiology Journal, 42(2), 101–118. https://doi.org/10.1080/01490451.2025.2545389

[15] Choi, S.-G., Chang, I., Lee, M., Lee, J.-H., Han, J.-T., & Kwon, T.-H. (2020). Review on geotechnical engineering properties of sands treated by microbially induced calcium carbonate precipitation and biopolymers. Construction and Building Materials, 246, 118415. https://doi.org/10.1016/j.conbuildmat.2020.118415

[16] Cristelo, N., Salifu, E., & Banerjee, A. (2026). Bio- and waste-based solutions as the next generation of soil stabilization techniques. In Transportation geotechnics for green, digital, and modern infrastructures (pp. 53–82). CRC Press.

[17] Cui, M. J., Lai, H. J., Hoang, T., & Chu, J. (2021). One-phase-low-pH enzyme induced carbonate precipitation method for soil improvement. Acta Geotechnica, 16, 481–489. https://doi.org/10.1007/s11440-020-01043-2

[18] Deylaghian, S., Nikooee, E., Seyedi, A., Niazi, A., & Nagel, T. (2025). Non-ureolytic EICP as a novel enzymatic pathway for sustainable soil stabilization. Scientific Reports, 15, 12345. https://doi.org/10.1038/s41598-025-13525-y

[19] Eryürük, K., Yenginar, Y., Özkan, İ., & Türk Dağı, H. (2025). Enhancing sandy soils of varying densities via microbially induced calcite precipitation. Advances in Civil and Architectural Engineering, 16(31), 165–179. https://doi.org/10.13167/2025.31.10

[20] Eryürük, Ş., Eryürük, K., & Katayama, A. (2025). Integrated bioprocess and response surface methodology-based design for hydraulic conductivity reduction using Sporosarcina pasteurii. Minerals, 15(11), 1215.

[21] Ezzat, S. M. (2023). A critical review of microbially induced carbonate precipitation for soil stabilization: The global experiences and future prospective. Pedosphere. Advance online publication. https://doi.org/10.1016/j.pedsph.2023.01.011

[22] Fouladi, A. S., Arulrajah, A., Chu, J., & Horpibulsuk, S. (2023). Application of microbially induced calcite precipitation technology in construction materials: A comprehensive review of waste stream contributions. Construction and Building Materials. https://doi.org/10.1016/j.conbuildmat.2023.131546

[23] Fu, Q., Zhang, Y., Lin, D., Zhao, R., & Cheng, X. (2023a). Mechanisms and applications of microbially induced calcite precipitation: A comprehensive review. Construction and Building Materials, 357, 129520. https://doi.org/10.1016/j.conbuildmat.2022.129520

[24] Fu, T., Saracho, A. C., & Haigh, S. K. (2023b). Microbially induced carbonate precipitation for soil strengthening: A comprehensive review. Biogeotechnics, Article 100002. https://doi.org/10.1016/j.bgtech.2023.100002

[25] Gowthaman, S., Iki, T., Ichinohe, A., Nakashima, K., & Kawasaki, S. (2022). Feasibility of bacterial-enzyme induced carbonate precipitation technology for stabilizing fine-grained slope soils. Frontiers in Built Environment, 8, Article 1044598. https://doi.org/10.3389/fbuil.2022.1044598

[26] Gowthaman, S., Nakashima, K., & Kawasaki, S. (2023). Field-scale bio-cementation of sandy soil using enzyme-induced carbonate precipitation with low-cost chemicals. Journal of Rock Mechanics and Geotechnical Engineering, 15(5), 1201–1214. https://doi.org/10.1016/j.jrmge.2022.12.010

[27] Haque, M. M., & Uddin, S. Z. (2024). A review on sustainable building materials and their role in enhancing US green infrastructure goals. Journal of Sustainable Development and Policy, 3(04), 65–100.

[28] Hommel, J., Akyel, A., Frieling, Z., Phillips, A. J., Gerlach, R., Cunningham, A. B., & Class, H. (2020). A numerical model for enzymatically induced calcium carbonate precipitation. Applied Sciences, 10(13), 4538. https://doi.org/10.3390/app10134538

[29] Ishara, S., Anand, R., Parihar, A., Reddy, M. S., & Goyal, S. (2024). Suitability and challenges of biomineralization techniques for ground improvement. International Journal of Environmental Research, 18(3), 45. https://doi.org/10.1007/s41742-024-00593-7

[30] Jamaldar, A., Asadi, P., Salimi, M., Payan, M., Ranjbar, P. Z., Arabani, M., & Ahmadi, H. (2024). Application of natural and synthetic fibers in bio-based earthen composites: A state-of-the-art review. Results in Engineering, Article 103732. https://doi.org/10.1016/j.rineng.2024.103732

[31] Jiang, X., Wang, H., & Wang, Y. (2025). Triaxial compression behavior and damage model of EICP-cemented calcareous sand. Geotechnical and Geological Engineering, 43, 77. https://doi.org/10.1007/s10706-024-03052-4

[32] Lajmiri, A., Sharafi, H., & Khayat, N. (2025). Evaluation of low-cost calcium sources for microbially induced calcite precipitation: Implications for sustainable bio-cementation. Results in Engineering, 25, 107638. https://doi.org/10.1016/j.rineng.2025.107638

[33] Li, J., Zhu, F., Wu, F., Chen, Y., Richards, J., Li, T., Li, P., Shang, D., Yu, J., Viles, H., & Guo, Q. (2024). Impact of soil density on biomineralization using EICP and MICP techniques for earthen sites consolidation. Journal of Environmental Management, 363, Article 121410. https://doi.org/10.1016/j.jenvman.2024.121410

[34] Liu, S., Du, K., Huang, W., Wen, K., & Amini, F. (2021). Improvement of erosion resistance of bio-cemented sandy soil using MICP and EICP techniques. Journal of Marine Science and Engineering, 9(8), 833. https://doi.org/10.3390/jmse9080833

[35] Ma, Y., Dong, X., Wang, Z., Liu, T., & Ma, W. (2025). Mechanical behavior and erosion resistance of desert soil stabilized with guar gum biopolymer. Bulletin of Engineering Geology and the Environment, 84, 442. https://doi.org/10.1007/s10064-025-04534-2

[36] Mehmood, M., Yosri, A. M., & Alzara, M. (2025). Basalt fiber reinforcement cementation with bio-inspired carbonate precipitation for stabilization of expansive soil. Scientific Reports, 15, 43561. https://doi.org/10.1038/s41598-025-31020-2

[37] Meng, H., Gao, Y., He, J., Qi, Y., & Hang, L. (2021). Microbially induced carbonate precipitation for wind erosion control of desert soil: Field-scale tests. Geoderma, Article 114723. https://doi.org/10.1016/j.geoderma.2020.114723

[38] Miftah, A., Khodadadi Tirkolaei, H., & Bilsel, H. (2020). Bio-precipitation of CaCO₃ for soil improvement: A review. IOP Conference Series: Materials Science and Engineering, 800, 012037. https://doi.org/10.1088/1757-899X/800/1/012037

[39] Mohammed, A. A., Nahazanan, H., Nasir, N. A. M., Huseien, G. F., & Saad, A. H. (2023). Calcium-based binders in concrete or soil stabilization: Challenges, problems, and calcined clay as partial replacement to produce low-carbon cement. Materials, 16(5), 2020.

[40] Ossai, R., Rivera, L., & Bandini, P. (2020). Experimental study to determine an EICP application method feasible for field treatment for soil erosion control. In Geo-Congress 2020: Biogeotechnics (GSP 320, pp. 96–103). American Society of Civil Engineers. https://doi.org/10.1061/9780784482834.023

[41] Payan, M., Sangdeh, M. K., Salimi, M., Ranjbar, P. Z., Arabani, M., & Hosseinpour, I. (2024). A comprehensive review on the application of microbially induced calcite precipitation technique in soil erosion mitigation as a sustainable and environmentally friendly approach. Results in Engineering. https://doi.org/10.1016/j.rineng.2024.103235

[42] Prajapati, N. K., Agnihotri, A. K., & Basak, N. (2023). Microbial induced calcite precipitation (MICP), a sustainable technique for stabilization of soil: A review. Materials Today: Proceedings. https://doi.org/10.1016/j.matpr.2023.07.303

[43] Putra, H., Yasuhara, H., Erizal, Sutoyo, & Fauzan, M. (2020). Review of enzyme-induced calcite precipitation as a ground-improvement technique. Infrastructures, 5(8), 66. https://doi.org/10.3390/infrastructures5080066

[44] Rahman, M. M., Hora, R. N., Ahenkorah, I., Beecham, S., Karim, M. R., & Iqbal, A. (2020). State-of-the-art review of microbial-induced calcite precipitation and its sustainability in engineering applications. Sustainability, 12(15), Article 6281. https://doi.org/10.3390/su12156281

[45] Raj, N., Selvakumar, S., & Muthukkumaran, K. (2026). A review of bio-based stabilization methods for expansive soils. Biogeotechnics, Article 100229. https://doi.org/10.1016/j.bgtech.2026.100229

[46] Ratna Atika Huwaida, Putra, H., Erizal, Qarinur, M., & Silitonga, E. M. R. (2026). Efficacy of sucrose and skimmed milk in enhancing sandy soil strength using the SCU-CP method. Civil Engineering and Architecture, 14(1), 300–316. https://doi.org/10.13189/cea.2026.140119

[47] Saif, A., Cuccurullo, A., Gallipoli, D., Perlot, C., & Bruno, A. W. (2022). Advances in enzyme induced carbonate precipitation and application to soil improvement: A review. Materials, 15(3), Article 950. https://doi.org/10.3390/ma15030950

[48] Sarma, S., & Mishra, A. K. (2024). Microbial-induced calcium carbonate precipitation: A potentially sustainable approach for geo-environmental challenges: A retrospection into the mechanism, influencing factors, characterization, and applications. Geomicrobiology Journal, 921–938. https://doi.org/10.1080/01490451.2024.2401887

[49] Shaivan, H. S., Yanez, V. R., Graddy, C. M. R., & Burns, S. E. (2025). Effect of natural carbonates on microbially induced calcite precipitation process. Scientific Reports, 15(1). https://doi.org/10.1038/s41598-025-97737-2

[50] Tang, C. S., Yin, L. Y., Jiang, N. J., Zhu, C., Zeng, H., Li, H., & Shi, B. (2020). Factors affecting the performance of microbial-induced carbonate precipitation treated soil: A review. Environmental Earth Sciences, 79, 94. https://doi.org/10.1007/s12665-020-8840-9

[51] Wang, Y., Sun, X., Miao, L., Wang, H., Wu, L., Shi, W., & Kawasaki, S. (2024). State-of-the-art review of soil erosion control by MICP and EICP techniques: Problems, applications, and prospects. Science of the Total Environment, Article 169016. https://doi.org/10.1016/j.scitotenv.2023.169016

[52] Xu, F., & Wang, D. (2023). Review on soil solidification and heavy metal stabilization by microbial-induced carbonate precipitation technology. Geomicrobiology Journal, 40(5), 503–518. https://doi.org/10.1080/01490451.2023.2208113

[53] Yu, M., Zhang, Z., Xu, C., Tian, S., & Tan, Z. (2026). Research progress on microbially induced calcium carbonate precipitation for reinforcing fractured rock masses. Coatings, 16(4), Article 413. https://doi.org/10.3390/coatings16040413

[54] Yusuf, M., Adewumi, J. R., & Olayiwola, S. A. (2025). Advances in sustainable geotechnical engineering: A review of bio-mediated soil stabilisation, cellular confinement systems, and waste-based soil improvements. Path of Science, 11(6), 7009–7021. https://doi.org/10.22178/pos.119-40

[55] Zhang, K., Tang, C. S., Jiang, N. J., Pan, X. H., Liu, B., Wang, Y. J., & Shi, B. (2023). Microbial induced carbonate precipitation technology: A review on the fundamentals and engineering applications. Environmental Earth Sciences, 82(9), 229.

[56] Zhang, X., Wang, H., Wang, Y., Wang, J., Cao, J., & Zhang, G. (2025). Improved methods, properties, applications and prospects of microbial induced carbonate precipitation treated soil: A review. Biogeotechnics, 3(1), Article 100123. https://doi.org/10.1016/j.bgtech.2024.100123

How to cite this paper

Odunewu Itunu Deborah, Ayininuola G. M., Akolade A. S., Adebayo K. J. "A Critical Review of MICP and EICP for Sustainable Soil Stabilization Mechanisms, Engineering Performance, Environmental Trade-Offs, and Implementation Challenges" Iconic Research And Engineering Journals Volume 9 Issue 11 2026 Page 4943-4959 https://doi.org/10.64388/IREV9I11-1718486
Odunewu Itunu Deborah, Ayininuola G. M., Akolade A. S., Adebayo K. J. "A Critical Review of MICP and EICP for Sustainable Soil Stabilization Mechanisms, Engineering Performance, Environmental Trade-Offs, and Implementation Challenges" Iconic Research And Engineering Journals, vol. 9, no. 11, May. 2026, doi: https://doi.org/10.64388/IREV9I11-1718486
Odunewu Itunu Deborah, Ayininuola G. M., Akolade A. S., Adebayo K. J. (2026). A Critical Review of MICP and EICP for Sustainable Soil Stabilization Mechanisms, Engineering Performance, Environmental Trade-Offs, and Implementation Challenges. Iconic Research And Engineering Journals, 9(11). doi: https://doi.org/10.64388/IREV9I11-1718486
Odunewu Itunu Deborah, Ayininuola G. M., Akolade A. S., Adebayo K. J. "A Critical Review of MICP and EICP for Sustainable Soil Stabilization Mechanisms, Engineering Performance, Environmental Trade-Offs, and Implementation Challenges" Iconic Research And Engineering Journals, vol. 9, no. 11, May. 2026. Crossref, https://doi.org/10.64388/IREV9I11-1718486
@article{1718486,
      author = {Odunewu Itunu Deborah, Ayininuola G. M., Akolade A. S., Adebayo K. J.},
      title = {A Critical Review of MICP and EICP for Sustainable Soil Stabilization Mechanisms, Engineering Performance, Environmental Trade-Offs, and Implementation Challenges},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {9},
      number = {11},
      pages = {4943-4959},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1718486.pdf},
      abstract = {Traditional cement- and lime-based soil stabilization's environmental constraints have driven the search for sustainable alternatives in geotechnical engineering. Bio-mediated methods like Microbial-Induced Calcite Precipitation (MICP) and Enzyme-Induced Calcite Precipitation (EICP) are gaining attention for their ability to improve soil properties through biologically induced calcium carbonate. This review covers recent advances (2020–2026) on mechanisms, performance, sustainability, and challenges of MICP and EICP for soil stabilization, based on about 120 peer-reviewed studies from major databases. EICP typically shows better penetration in fine soils due to no bacterial size limits, while MICP offers stronger bonding in granular soils from bacterial nucleation. Significant progress has been made in strength, permeability, erosion, and swelling, but issues like non-uniform treatment, ammonia release, brittleness, cost, and durability remain barriers. Tropical lateritic soils are understudied despite their importance in developing countries. Emerging techniques like non-ureolytic methods, fiber systems, nanomaterials, and waste reagents show promise for sustainability and field use. Future work should focus on field validation, durability testing, eco-friendly precipitation, and standard design to move bio-stabilization from research to real-world infrastructure.},
      keywords = {Microbial-Induced Calcite Precipitation, Enzyme-Induced Calcite Precipitation, Biomineralization, Sustainable Soil Stabilization, Bio-Cementation.},
      month = {May},
      doi = {https://doi.org/10.64388/IREV9I11-1718486}
  }