Pharmacology, Phytochemistry, Toxicology and Micropropagation of Spilanthes acmella (L.) Murr.: A Comprehensive Review

References

[1] Dubey, S., Maity, S., Singh, M., Saraf, S. A., & Saha, S. (2013). Phytochemistry, pharmacology and toxicology of Spilanthes acmella: A review. Advances in Pharmacological Sciences, 2013, 423750.

[2] Aktar, A., Bhuia, S., Molla, S., Chowdhury, R., Sarkar, C., Shahariar, M. A., Roy, P., Reiner, Ž., Sharifi-Rad, J., Calina, D., et al. (2024). Pharmacological and phytochemical review of Acmella oleracea: A comprehensive analysis of its therapeutic potential. Discover Applied Sciences, 6, 412.

[3] Jansen RK (1985) The Systematics of Acmella (Asteraceae-Heliantheae). Systematic Botany Monographs 8: 1-115.

[4] Paulraj, J., Govindarajan, R., & Palpu, P. (2013). The genus Spilanthes: Ethnopharmacology, phytochemistry, and pharmacological properties. Advances in Pharmacological Sciences, 2013, 510298.

[5] Lee, B.-W. (2026). Botany, ethnopharmacology, phytochemistry, and biological activities of Acmella oleracea: A comprehensive review. Molecules, 31(4), 677.

[6] Nakatani, N., & Nagashima, M. (1992). Pungent alkamides from Spilanthes acmella L. var. oleracea. Bioscience, Biotechnology, and Biochemistry, 56(5), 759–762.

[7] Prachayasittikul, S., Suphapong, A., Worachartcheewan, A., Lawung, R., Ruchirawat, S., & Prachayasittikul, V. (2009). Bioactive metabolites from Spilanthes acmella Murr. Molecules, 14(2), 850–867.

[8] Mukharya, D. K., & Ansari, A. H. (1987). Olean-12-en-3-O-β-D-galactopyranosyl-(1→4)-O-α-L-rhamnopyranoside: A new triterpenoidal saponin from the roots of Spilanthes acmella. Indian Journal of Chemistry Section B, 26B, 87–89.

[9] Spelman, K., Depoix, D., McCray, M., Mouray, E., & Grellier, P. (2011). The traditional medicine Spilanthes acmella, and the alkylamides spilanthol and undeca-2E-ene-8,10-diynoic acid isobutylamide, demonstrate in vitro and in vivo antimalarial activity.

[10] Baruah, R. N., & Leclercq, P. A. (1993). Characterization of the essential oil from flower heads of Spilanthes acmella. Journal of Essential Oil Research, 5(6), 693–695.

[11] Chakraborty, A., Devi, R.K.B., Rita, S., Sharatchandra, K.H., & Singh, T.I. (2010). Preliminary studies on anti-inflammatory and analgesic activities of Spilanthes acmella. Indian Journal of Pharmacology, 42(6), 382–384.

[12] Wongsawatkul, O., Prachayasittikul, S., Isarankura-Na-Ayudhya, C., Satayavivad, J., Ruchirawat, S., & Prachayasittikul, V. (2008). Vasorelaxant and antioxidant activities of Spilanthes acmella. Phytomedicine, 15(12), 1114–1124.

[13] Spelman, K., Depoix, D., McCray, M., Mouray, E., & Grellier, P. (2011). Journal of Ethnopharmacology, 132, 424–432.

[14] Saraf, D.K., & Dixit, V.K. (2002). Asian Journal of Experimental Sciences, 16, 9–19.

[15] Ekanem, A.P., Wang, M., Simon, J.E., & Moreno, D.A. (2007). Phytotherapy Research, 21, 1253–1255.

[16] Sharma, A., Kumar, V., Rattan, R. S., Kumar, N., & Singh, B. (2012). Insecticidal toxicity of spilanthol from Spilanthes acmella Murr. against Plutella xylostella (Lepidoptera: Plutellidae). American Journal of Plant Sciences, 3(11), 1568–1572.

[17] Kadir, H. A., Zakaria, M. B., Kechil, A. A., & Azirun, M. S. (1989). Toxicity and electrophysiological effects of Spilanthes acmella Murr. Pesticide Science, 25(4), 329–335.

[18] Ponpornpisit, A., Pirarat, N., Suthikrai, W., & Binwihok, A. (2011). Toxicity of Spilanthes acmella on zebrafish embryos. Thai Journal of Veterinary Medicine, 41(4), 543–547.

[19] Saraf, D. K., & Dixit, V. K. (2002). Spilanthes acmella Murr.: Study on its extract spilanthol as a larvicidal compound. Asian Journal of Experimental Sciences, 16(1–2), 9–19.

[20] Sharma, A., Kumar, V., Rattan, R. S., Kumar, N., & Singh, B. (2012). Insecticidal toxicity of spilanthol from Spilanthes acmella Murr. against Plutella xylostella. American Journal of Plant Sciences, 3(11), 1568–1572.

[21] Haw, A. B., & Keng, C. L. (2003). Micropropagation of Spilanthes acmella L., a bioinsecticide plant, through proliferation of multiple shoots. Journal of Applied Horticulture, 5(2), 65–68.

[22] Sana, H., & Rani, A. S. (2017). Micropropagation of Spilanthes acmella Murr. from nodal segment and apical shoot tip cultures. Indian Journal of Scientific Research, 13(1), 92–98.

[23] Singh, M., & Chaturvedi, R. (2012). In vitro regeneration of Spilanthes acmella through leaf-derived callus cultures. Plant Tissue Culture and Biotechnology, 22(1), 35–42.

[24] Sana, H. (2018). Comparative evaluation of bioactive compounds and larvicidal efficacy of micropropagated Spilanthes acmella plants. Industrial Crops and Products, 112, 842–849.

@article{1718700,
      author = {Grace Thomas, Rani R Nair, Chithra Gopinath},
      title = {Pharmacology, Phytochemistry, Toxicology and Micropropagation of Spilanthes acmella (L.) Murr.: A Comprehensive Review},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {9},
      number = {12},
      pages = {747-753},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1718700.pdf},
      abstract = {Spilanthes acmella (L.) Murr., commonly known as the toothache plant or eyeball plant, is an important medicinal herb belonging to the family Asteraceae. The plant is widely distributed in tropical and subtropical regions and has been extensively used in traditional medicine for the treatment of toothache, oral infections, inflammation, rheumatism, fever, and skin diseases. The therapeutic potential of S. acmella is mainly attributed to the presence of bioactive alkamides, particularly spilanthol, along with flavonoids, phenolics, triterpenoids, phytosterols, coumarins, and essential oils. Numerous pharmacological investigations have demonstrated its antimicrobial, anti-inflammatory, analgesic, antioxidant, antimalarial, hepatoprotective, anti-obesity, vasorelaxant, and insecticidal activities. Recent toxicological studies suggest that the plant exhibits relatively low toxicity at therapeutic doses. However, increasing commercial demand and habitat depletion have raised concerns regarding its conservation. Consequently, in vitro micropropagation techniques have been developed to facilitate large-scale cultivation and sustainable utilization. This review summarizes the ethnomedicinal uses, phytochemical constituents, pharmacological activities, toxicological profile, and advances in micropropagation of S. acmella, highlighting its significance as a promising medicinal and industrial plant.},
      keywords = {Spilanthes Acmella, Spilanthol, Alkamides, Medicinal Plant, Pharmacology, Toxicology, Micropropagation},
      month = {June},
      doi = {https://doi.org/10.64388/IREV9I12-1718700}
  }