Isolation of Genomic DNA from E. coli

• Author(s): Krishna Singh Raghuvanshi; Dr. Sohni Jain
• Paper ID: 1719655
• Page: 1058-1061
• Published Date: 13-07-2026
• Published In: Iconic Research And Engineering Journals
• Publisher: IRE Journals
• e-ISSN: 2456-8880
• Volume/Issue: Volume 10 Issue 1 July-2026
• DOI: https://doi.org/10.64388/IREV10I1-1719655

Abstract

Isolation of nucleic acid, especially DNA, is very common and basic steps in molecular biology. For the isolation of these can be done from different biological materials for the different purpose like study, research or medical and etc. As per the objective “Isolation of genomic DNA from Escherichia coli (E. coli)” the easiest, best, high quality DNA isolation and cost-effective method has prominently four steps like cell lysis, enzymatic treatment, differential solubility and precipitation. For performing these crucial steps there are many old conventional protocols as well as modern and easy protocol are also there. But for the high precision and accuracy there are kits like GeNie also used in which there are less chances of contamination as well as time saviour and ready to use reagents and enzymes are present. The successful isolation and visualization of DNA are confirmed by the appearance of clear, well-defined bands, indicating high-quality genomic DNA. This method provides a reliable and straightforward means of isolating genomic DNA from E. coli for further molecular applications.

Keywords

DNA Extraction, Escherichia Coli, Electrophoresis, Genie Kit

Citations

IRE Journals:
Krishna Singh Raghuvanshi, Dr. Sohni Jain "Isolation of Genomic DNA from E. coli" Iconic Research And Engineering Journals Volume 10 Issue 1 2026 Page 1058-1061 https://doi.org/10.64388/IREV10I1-1719655

IEEE:
Krishna Singh Raghuvanshi, Dr. Sohni Jain "Isolation of Genomic DNA from E. coli" Iconic Research And Engineering Journals, vol. 10, no. 1, Jul. 2026, doi: https://doi.org/10.64388/IREV10I1-1719655

APA:
Krishna Singh Raghuvanshi, Dr. Sohni Jain (2026). Isolation of Genomic DNA from E. coli. Iconic Research And Engineering Journals, 10(1). doi: https://doi.org/10.64388/IREV10I1-1719655

MLA:
Krishna Singh Raghuvanshi, Dr. Sohni Jain "Isolation of Genomic DNA from E. coli" Iconic Research And Engineering Journals, vol. 10, no. 1, Jul. 2026. Crossref, https://doi.org/10.64388/IREV10I1-1719655

BibTeX

@article{1719655,
author = {Krishna Singh Raghuvanshi, Dr. Sohni Jain},
title = {Isolation of Genomic DNA from E. coli},
journal = {Iconic Research And Engineering Journals},
year = {2026},
volume = {10},
number = {1},
pages = {1058-1061},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1719655.pdf},
abstract = {Isolation of nucleic acid, especially DNA, is very common and basic steps in molecular biology. For the isolation of these can be done from different biological materials for the different purpose like study, research or medical and etc. As per the objective “Isolation of genomic DNA from Escherichia coli (E. coli)” the easiest, best, high quality DNA isolation and cost-effective method has prominently four steps like cell lysis, enzymatic treatment, differential solubility and precipitation. For performing these crucial steps there are many old conventional protocols as well as modern and easy protocol are also there. But for the high precision and accuracy there are kits like GeNie also used in which there are less chances of contamination as well as time saviour and ready to use reagents and enzymes are present. The successful isolation and visualization of DNA are confirmed by the appearance of clear, well-defined bands, indicating high-quality genomic DNA. This method provides a reliable and straightforward means of isolating genomic DNA from E. coli for further molecular applications.},
keywords = {DNA Extraction, Escherichia Coli, Electrophoresis, Genie Kit},
month = {July}
}