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Isolation of Genomic DNA from E. coli

Krishna Singh Raghuvanshi Dr. Sohni Jain

Subject area: Biological & Medical Sciences  ·  Area of research: Molecular Biology

DOI: https://doi.org/10.64388/IREV10I1-1719655

Abstract

Isolation of nucleic acid, especially DNA, is very common and basic steps in molecular biology. For the isolation of these can be done from different biological materials for the different purpose like study, research or medical and etc. As per the objective “Isolation of genomic DNA from Escherichia coli (E. coli)” the easiest, best, high quality DNA isolation and cost-effective method has prominently four steps like cell lysis, enzymatic treatment, differential solubility and precipitation. For performing these crucial steps there are many old conventional protocols as well as modern and easy protocol are also there. But for the high precision and accuracy there are kits like GeNie also used in which there are less chances of contamination as well as time saviour and ready to use reagents and enzymes are present. The successful isolation and visualization of DNA are confirmed by the appearance of clear, well-defined bands, indicating high-quality genomic DNA. This method provides a reliable and straightforward means of isolating genomic DNA from E. coli for further molecular applications.

Keywords

DNA Extraction, Escherichia Coli, Electrophoresis, Genie Kit

References

[1] Barnard, T. G., et al. (2011). A rapid and low-cost DNA extraction method for isolating Escherichia coli DNA from animal stools. African Journal of Biotechnology, 10(8), 1485–1490. http://www.academicjournals.org

[2] Oslaene, A. B., et al. (2022). Comparing protocols for DNA extraction from Escherichia coli: Analysis of purity and concentration by gel electrophoresis. Baghdad Journal of Biochemistry and Applied Biological Sciences, 3(2), 133–144. https://doi.org/10.47419/bjbabs.v3i02.123

[3] Moore, E., et al. (2004). Simplified protocols for the preparation of genomic DNA from bacterial cultures. Molecular Microbial Ecology Manual, 2(1.01), 3–18.

[4] Miranda, R. R., et al. (2011). Detergents and ultrasound evaluation for genomic DNA isolation of food bacteria. Revista Brasileira de Tecnologia Agroindustrial, 5(1), 398–407.

[5] Bonatto, F. D. A., et al. (2014). Comparison of DNA extraction protocols in bacteria Escherichia coli and Pseudomonas spp. Revista Eletrônica de Análises Clínicas, 2(1), 1–11.

[6] Eusébio, A., et al. (2016). Escherichia coli in community urinary tract infections: Commensal or pathogenic? Acta Urológica Portuguesa, 33(2), 37–42. https://doi.org/10.1016/j.acup.2016.02.002

[7] De Sousa Alves, P. G., et al. (2021). Optimization of Gram-positive bacteria DNA extraction protocols and purity and concentration analysis by gel electrophoresis. Revista Interfaces (Juazeiro do Norte), 9(2), 1133–1140. https://doi.org/10.16891/944

[8] Tan, S. C., & Yiap, B. C. (2009). DNA, RNA, and protein extraction: The past and the present. Journal of Biomedicine and Biotechnology, 2009, Article 574398. https://doi.org/10.1155/2009/574398

How to cite this paper

Krishna Singh Raghuvanshi, Dr. Sohni Jain "Isolation of Genomic DNA from E. coli" Iconic Research And Engineering Journals Volume 10 Issue 1 2026 Page 1058-1061 https://doi.org/10.64388/IREV10I1-1719655
Krishna Singh Raghuvanshi, Dr. Sohni Jain "Isolation of Genomic DNA from E. coli" Iconic Research And Engineering Journals, vol. 10, no. 1, Jul. 2026, doi: https://doi.org/10.64388/IREV10I1-1719655
Krishna Singh Raghuvanshi, Dr. Sohni Jain (2026). Isolation of Genomic DNA from E. coli. Iconic Research And Engineering Journals, 10(1). doi: https://doi.org/10.64388/IREV10I1-1719655
Krishna Singh Raghuvanshi, Dr. Sohni Jain "Isolation of Genomic DNA from E. coli" Iconic Research And Engineering Journals, vol. 10, no. 1, Jul. 2026. Crossref, https://doi.org/10.64388/IREV10I1-1719655
@article{1719655,
      author = {Krishna Singh Raghuvanshi, Dr. Sohni Jain},
      title = {Isolation of Genomic DNA from E. coli},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {10},
      number = {1},
      pages = {1058-1061},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1719655.pdf},
      abstract = {Isolation of nucleic acid, especially DNA, is very common and basic steps in molecular biology. For the isolation of these can be done from different biological materials for the different purpose like study, research or medical and etc. As per the objective “Isolation of genomic DNA from Escherichia coli (E. coli)” the easiest, best, high quality DNA isolation and cost-effective method has prominently four steps like cell lysis, enzymatic treatment, differential solubility and precipitation. For performing these crucial steps there are many old conventional protocols as well as modern and easy protocol are also there. But for the high precision and accuracy there are kits like GeNie also used in which there are less chances of contamination as well as time saviour and ready to use reagents and enzymes are present. The successful isolation and visualization of DNA are confirmed by the appearance of clear, well-defined bands, indicating high-quality genomic DNA. This method provides a reliable and straightforward means of isolating genomic DNA from E. coli for further molecular applications.},
      keywords = {DNA Extraction, Escherichia Coli, Electrophoresis, Genie Kit},
      month = {July},
      doi = {https://doi.org/10.64388/IREV10I1-1719655}
  }