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1720021 Vol 10 · Issue 1 Download Paper

Green Synthesis, Characterization and Evaluation of Antimicrobial Activity of Copper Oxide Nanoparticles Using Natural Extract of Ginger (Zingiber officinale)

Dr Surendra Kumar Sonwane Haribati Maravi Ishwar Singh Markam Mitali Pardhi Vandana Markam

Subject area: Biological & Medical Sciences  ·  Area of research: Herbal Antibiotics

DOI: 10.64388/IREV10I1-1720021

Abstract

The green synthesis of CuO nanoparticles using plant extracts, specifically ginger (Zingiber officinale) was studied for its affordability and eco-friendly traits. The CuO NPs were analyzed via Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (FESEM-EDX) and TEM. Their antimicrobial effectiveness was evaluated against two bacterial strains Escherichia coli ( E. coli) and Staphylococcus aureus ( S. aureus) and two fungal species Fusarium oxysporum (F. oxysporum) and Fusarium graminearum (F.graminearum) using the disk diffusion technique. The biosynthesized CuO nanoparticles were evaluated alongside chemically synthesized CuO nanoparticles to assess how the synthesis method influences their structural, optical, morphological properties, as well as their antibacterial and antifungal efficacy. The topological characteristics including dimensions, shapes, aggregation behavior, and basic structural arrangements of green-synthesized CuO nanoparticles are critical determinants of their biological activity. Investigations serve as valuable methods for studying these properties. TEM analysis confirmed that the biosynthesized CuO nanoparticles have a spherical shape. Particle size distribution analysis showed a size range of 4 to 28 nm, with an average diameter of 16.2 nm.

Keywords

Nanocomposites, Green Synthesis, Natural, Extracts Ginger, Antibacterial and Antifungal Activity.

References

[1] Fifere N., Airinei A., Doroftei F., Ardeleanu T.S., Dobromir, M., Timpu D., Ursu E. L., Phytomediated-Assisted Preparation of Cerium Oxide Nanoparticles Using Plant Extracts and Assessment of Their Structural and Optical Properties., Int. J. Mol. Sci. 2023, 24, 8917, 1-21.

[2] Bayda S., Adeel M., Tuccinardi T., Cordani M., Rizzolio F., The History of Nanoscience and Nanotechnology: From Chemical-Physical Applications to Nanomedicine. Molecules, 2019, 25, 112, 1-15 .

[3] Thakur N., Manna P., Das J., Synthesis and biomedical applications of nanoceria, a redox active nanoparticle, J. Nanobiotechnol., 2019, 17, 84, 1-27.

[4] Selvakesavan R.K., Franklin G., Prospective Application of Nanoparticles Green Synthesized Using Medicinal Plant Extracts as Novel Nanomedicines, Nanotechnol. Sci. Appi., 2021, 14, 179–195.

[5] Nadeem M., Khan R., Afridi K., Nadhman A., Ullah S., Faisal S., Mabood Z. U., Hano C., Abbasi B.H., Green Synthesis of Cerium Oxide Nanoparticles (CeO2 NPs) and Their Antimicrobial Applications, A Review., Int. J. Nanomed., 2020, 15, 5951–5961.

[6] Tenchov R., Hughes K.J., Ganesan M., Iyer K. A., Ralhan K., Lotti Diaz L. M., Bird R. E., Ivanov J. M., Zhou Q. A., Transforming medicine: cutting-edge applications of nanoscale materials in drug delivery, ACS Nano., 2025, 19(4), 4011–38.

[7] Lahlali R., Boutagayout A., Taoussi M., Hamdani A., Adiba A., Assouguem A., Kouighat M., Farhaoui A., Nanoparticles pioneering solutions for plant disease management. sustainable era of nanomaterials: opening up a path for a green future., Springer, 2025, 345–78,1-20 .

[8] Tijani N. A., Hokello J., Eilu E., Akinola S. A., Afolabi A. O., Makeri D., Lukwago T. W., Mutuku I. M., Mwesigwa A., Baguma A., Metallic nanoparticles: a promising novel therapeutic tool against antimicrobial resistance and spread of super¬bugs. Biometals., 2025, 38(1), 55–88.

[9] Soliman M. K., Abu Elghait M., Salem S. S., Azab M. S., Multifunctional properties of silver and gold nanoparticles synthesis by Fusarium pseudonygamai. Biomass Convers Biorefinery., 2024, 14(22):28253–70.

[10] Alruhaili M. H., Selim S., Adly E., Alharbi M. T., Al-ahmadi B. M. , Almehayawi M. S., Al Jaouni S. K. , Salem S. S., Abu-Hussien S. H., Green synthesis of silver nanoparticles from Bacillus subtilis-mediated feather hydrolysate: antimicrobial, larvicidal against culex pipiens, and anticancer activities., Bioresources Bioprocess., 2025, 12(1), 116.

[11] Jiang H., Li L., Li Z., Chu X., Metal-based nanoparticles in antibacterial applica¬tion in biomedical field: Current development and potential mechanisms. Biomed Microdevices. 2024, 26(1), 12.

[12] Abu-Elghait M., Soliman M. K., Azab M. S., Salem S. S., Response surface methodology: Optimization of myco-synthesized gold and silver nanopar¬ticles by Trichoderma saturnisporum. Biomass Convers Biorefinery., 2025, 15(3), 4211–24.

[13] Soliman M. K., Hashem A. H., Al-Askar A. A., Abd-Elgayed G., Salem S. S., Green synthesis of silver nanoparticles from Bauhinia variegata and their biological applications., Green Process Synthesis., 2024, 13(1), 20240099.

[14] Malaikozhundan B., Mohandoss S., Krishnamoorthi R., Bharathi P. V., Palanisamy S, Vinodhini J., Enhanced bactericidal, antibiofilm and antioxidative response of Lawsonia inermis leaf extract synthesized ZnO NPs loaded with commer¬cial antibiotic., Bioprocess Biosyst. Eng., 2024, 47(8), 1241–57.

[15] Vinothini P., Malaikozhundan B., Krishnamoorthi R., Senthamarai M. D., Shanthi D., Potential inhibition of biofilm forming bacteria and fungi and DPPH free radicals using Tamarindus indica fruit extract assisted iron oxide nanoparticle. Inorg. Chem. Commun., 2023, 156, 111206.

[16] Yousef A., Salem S.S., Ragab A., El Shahaly O.M., Rateb H.O., El-Esawy R.A., Elakraa A.M., Abd E., Hamid E. S., Ibrahim S., Mentha spicata-mediated silver nanopar¬ticles for combating Streptococcus mutans and oral cancer cells., Sci Rep., 2025, 15(1), 38474.

[17] Almuhayawi M. S., Alruhaili M. H., Soliman M. K., Tarabulsi M. K., Ashy R. A., Saddiq A. A., Selim S., Alruwaili Y., Salem S. S., Investigating the in vitro antibacterial, antibio-film, antioxidant, anticancer and antiviral activities of zinc oxide nanoparticles biofabricated from Cassia javanica., PLoS ONE., 2024, 19(10), e0310927.

[18] Soliman M. K., Salem S. S., Abu-Elghait M., Azab M. S., Biosynthesis of silver and gold nanoparticles and their efficacy towards antibacterial, antib¬iofilm, cytotoxicity, and antioxidant activities., Appl Biochem Biotechnol., 2023, 195(2), 1158–83.

[19] Dubey S., Virmani T., Yadav S. K., Sharma A., Kumar G., Alhalmi A., Breaking barriers in eco-friendly synthesis of plant‐mediated metal/metal oxide/bimetallic nanoparticles: antibacterial, anticancer, mechanism elucidation, and versatile utilizations. J Nanomaterials., 2024, 2024(1), 9914079.

[20] Luo L., Huang W., Zhang J., Yu Y., Sun T., Metal-based nanoparticles as antimicro¬bial agents: a review. ACS Appl Nano Mater., 2024, 7(3), 2529–45.

[21] Salem S. S., Soliman M. K., Azab M. S., Abu-Elghait M., Optimization growth conditions of Fusarium pseudonygamai for myco-synthesized gold and silver nanoparticles using response surface methodology., Bio. NanoScience. 2024, 1–16.

[22] Jiang H., Li L., Li Z., Chu X., Metal-based nanoparticles in antibacterial applica¬tion in biomedical field: Current development and potential mechanisms. Biomed Microdevices. 2024, 26(1), 12.

[23] Soliman M. K., Salem S. S., Comparative evaluation of antimicrobial, antibiofilm, antioxidant, antiviral, and antidiabetic activities of copper oxide nanoparticles biofabricated via Opuntia ficus indica. Sci Rep., 2025, 15(1), 24823.

[24] Malaikozhundan B., Lakshmi V. N., Krishnamoorthi R., Copper oxide nanopar¬ticles using Mentha spicata leaves as antibacterial, antibiofilm, free radical scavenging agent and efficient photocatalyst to degrade methylene blue dyes., Mater Today Commun., 2022, 33, 104348.

[25] Abuthahir A. K., Malaikozhundan B., Mohandoss S., Manoharadas S., Palanisamy S., Rajan D. K., Krishnamoorthi R., Orange fruit peel biowaste mediated green synthesis of CaO NPs and their antibacterial, radical scavenging activity and ecotoxicity., Bioorg Chem. 2025, 108835.

[26] Kassem A. A., Abdelhamid H.N., Fouad D. M., Ibrahim S. A., Hydrogenation reduc¬tion of dyes using metal-organic oxide nanoparticles for anticancer activity and antibacte¬rial effects against Streptococcus mutans using molecular docking. Sci Rep. 2025;15(1):40921.

[27] Singh A, Singh N., framework-derived CuO@ C. Microporous Mesoporous Mater., 2020, 305, 110340.

[28] Salem S. S., Yousef A., Abd E.l. Hamid E. S., Ibrahim S., Elbawab R.H., Evaluation of eco-friendly copper oxide nanoparticles for anticancer activity and antibacterial effects against Streptococcus mutans using molecular docking., Sci Rep., 2025 Nov 20, 15(1), 40921.

[29] Hussain I., Singh H., Effect of biologically synthesized copper oxide nanoparticles on metabolism and antioxidant activity to the crop plants Solanum lycopersicum and Brassica oleracea var. botrytis., J Biotechnol., 2017, 262, 11–27.

[30] Spooner A., Chen E., Sowmya A., Sachdev P., Kochan N. A., Trollor J., Brodaty H. A. comparison of machine learning methods for survival analysis of high-dimensional clinical data for dementia prediction., Sci. Rep., 2020, 10(1), 20410.

[31] Makarov V., Love A., Sinitsyna O., Makarova S., Yaminsky I., Taliansky M., Kalinina N., Green nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae, 2014, 6(1), 35–44.

[32] Mao Q-Q, Xu X-Y, Cao S-Y, Gan R-Y, Corke H., Beta T., Li H-B. Bioactive com¬pounds and bioactivities of ginger (Zingiber officinale Roscoe)., Foods. 2019, 8(6), 185.

[33] Ali E. M., Abdalameer N. K., Khalaph K. A., Hadi F., Ramadhan A. O., Green reduction approach for the synthesis of copper oxide nanoparticles using ginger extract and evaluation of their free radical scavenging activity., Eur Phys J Plus., 2025;140(9), 928.

[34] Hazrati R., Zare N., Asghari R., Sheikhzadeh P., Johari-Ahar M., Biologically synthesized CuO NPs induce physiological, metabolic, and molecular changes in the hazel cell cultures., Appl Microbiol Biotechnol., 2022, 106(18), 6017–31.

[35] Mariadoss A. V. A., Saravanakumar K., Sathiyaseelan A., Venkatachalam K., Wang M-H., Folic acid functionalized starch encapsulated green synthesized copper oxide nanoparticles for targeted drug delivery in breast cancer therapy., Int J Biol Macromol., 2020, 164, 2073–84.

[36] Almasi H., Jafarzadeh P., Mehryar L., Fabrication of novel nanohybrids by impregnation of CuO NPs into bacterial cellulose and chitosan nanofibers: Characterization, antimicrobial and release properties. Carbohydr Polym., 2018, 186, 273–81.

[37] Gad E. S., Salem S. S., Selim S., Almuhayawi M. S., Alruhaili M. H. , Al Jaouni S. K., Saddiq A. A., Owda M. E., A comprehensive study on characterization of biosynthesized copper-oxide nanoparticles, their capabilities as anticancer and antibacterial agents, and predicting optimal docking poses into the cavity of S. aureus DHFR. PLoS ONE. 2025, 20(4), e0319791.

[38] Faisal S., Jan H., Abdullah, Alam I., Rizwan M., Hussain Z., Sultana K., Ali Z., Uddin M. N., In vivo analgesic, anti-inflammatory, and anti-diabetic screening of Bacopa monnieri-synthesized copper oxide nanoparticles., ACS omega., 2022, 7(5), 4071–82.

[39] Hussein A. S., Hashem A. H., Salem S. S., Mitigation of the hyperglycemic effect of streptozotocin-induced diabetes albino rats using biosynthesized copper oxide nanoparticles, Biomol Concepts., 2023, 14(1).

[40] Rehana D., Mahendiran D., Kumar R. S., Rahiman A. K., Evaluation of antioxi¬dant and anticancer activity of copper oxide nanoparticles synthesized using medicinally important plant extracts, Biomed Pharmacother., 2017, 89, 1067–77.

[41] Biswal H. J., Vundavilli P.R., Mondal K., Shetti N. P., Gupta A., ZnO/CuO nanostructures anchored over Ni/Cu tubular films via pulse electrodeposition for photocatalytic and antibacterial applications, Mat. Sci. Energy Technol., 2023, 6, 237–251.

[42] Xu Z., Wang Y., Li S., et al., Advances of functional nanomaterials as either therapeutic agents or delivery systems in the treatment of periodontitis, Biomater. Adv., 2025, 175 214326.

[43] Nascimento G.G., Alves-Costa S., Romandini M., Burden of severe periodontitis and edentulism in 2021, with projections up to 2050: the global burden of disease 2021 study, J. Periodontal Res., 2024, 59 (5) 823–867.

[44] Benzian H., Watt R., Makino Y., et al., WHO calls to end the global crisis of oral health, Lancet , 2022, 400 (10367) 1909–1910.

[45] Yan F., Liu D., Zhao B., et al., Intervening with nanozymes in aging-related diseases: strategies for restoring mitochondrial function, Biomater. Adv., 2025, 169 214193.

[46] Wongpan A., Nuchpun S., Tana-Atsawapon N., et al., Engineered riboflavin- cerium oxide nanoparticles for enhanced phototoxicity toward triple-negative breast cancer cells, Nanoscale Adv., 2025, 7 (22) 7196–7208.

[47] Zhao M., Yang J., Liang J., et al., Emerging nanozyme therapy incorporated into dental materials for diverse oral pathologies, Dent. Mater., 2024, 40 (11), 1710–1728.

[48] Squarzoni C., N. Kania, M. Dearg, et al., Nanoceria as an efficient and cost-effective metal-free catalyst for the oxidation of alcohols, ACS Sustain. Chem. Eng., 2025, 13 (39), 16348–16363.

[49] Othman A., Gowda A., Andreescu D., et al., Two decades of ceria nanoparticle research: structure, properties and emerging applications, Mater. Horiz. 2024 11 (14), 3213–3266.

[50] Pan T., Li L., Wang S., et al., Biocomposite containing polyetherketoneketone and heterojunction of NaNbOX@CeO2 with improved piezoelectricity and nanozyme activity for killing bacteria and enhancing osteoblastic differentiation, Biomater. Adv., 2026, 179, 214491.

[51] Selvam K., Albasher G., Alamri O., Sudhakar C., Selvankumar T., Vijayalakshmi S., Vennila L., Enhanced photocatalytic activity of novel Canthium coromandelicum leaves based copper oxide nanoparticles for the degradation of textile dyes, Environ. Res., 2022, 211 113046.

[52] Shah I.H., Ashraf M., ISabir .A., Manzoor M.A., Malik M. S., Gulzar S., Ashraf F., Iqbal J., QNiu ., Zhang Y., Green synthesis and Characterization of Copper oxide nanoparticles using Calotropis procera leaf extract and their different biological potentials, J. Mol. Struct., 2022, 1259, 132696.

[53] Karthik C., Suresh S., Mirulalini S., Kavitha S., A FTIR approach of green synthe¬sized silver nanoparticles by Ocimum sanctum and Ocimum gratissimum on mung bean seeds. Inorg Nano-Metal Chem., 2020, 50(8), 606–12.

[54] Teklu B., Kumari S., Vidavalur S., Results in Chemistry Green synthesis of copper oxide nanoparticles using Balanites aegyptiaca stem bark extract and investigation of antibacterial activity. Results Chem., 2023, 6, 101152.

[55] Manasa D. J., Chandrashekar K. R., Kumar D. J. M., Niranjana M., Mussaenda frondosa L. mediated facile green synthesis of Copper oxide nanoparticles-Characterization, photocatalytic and their biological investigations, Arab. J. Chem., 2021, 14, 103184.

[56] Modan E.M., Schiopu A., Moga S.G., Negrea D. A., Istrate D. Ciuca I., Oproescu M. Advanced Copper Oxide Chemical and Green Synthesis: Characterization and Antibacterial Evaluation, Crystals, 2025, 15, 7.

[57] Saha N., Trivedi P., Dutta Gupta S., Surface plasmon resonance (SPR) based optimization of biosynthesis of silver nanoparticles from rhizome extract of Curculigo orchioides Gaertn and its antioxidant potential, J. Cluster. Sci., 2016, 27, 1893–912.

[58] Sakugawa K., Ikeda A., Takemura A., Ono H., Simplified method for estimation of composition of alginates by FTIR, J. Appl. Polym. Sci., 2004, 93(3), 1372–7.

[59] Dangi Y. R., Bediako J. K., Lin X., Choi J-W., Lim C-R., Song M-H., Han M., Yun Y-S., Polyethyleneimine impregnated alginate capsule as a high capacity sorbent for the recovery of monovalent and trivalent gold, Sci. Rep., 2021, 11(1), 17836.

[60] Al-Qasmi N. Facial eco-friendly synthesis of copper oxide nanoparticles using chia seeds extract and evaluation of its electrochemical activity, Processes., 2021, 9(11), 2027.

[61] Sabeena G., Rajaduraipandian S., Pushpalakshmi E., Alhadlaq H.A., Mohan R., Annadurai G., Ahamed, M. Green and chemical synthesis of CuO nanoparticles: A comparative study for several in vitro bioactivities and in vivo toxicity in zebrafish embryos, J. King Saud Univ. Sci., 2022, 34, 102092.

[62] Puri A., Antiadherence and Antimicrobial Properties of Copper Oxide Nanoparticles against Streptococcus mutans,Staphylococcus aureus, and Candida albicans on Orthodontic Brackets: An in Vitro Study, Iran. J. Orthod., 2025, 20, 1–16.

[63] Ssekatawa K., Byarugaba D. K., Angwe M. K., Wampande E. M., Ejobi F., Nxumalo E., Maaza M., Sackey J., Kirabira J. B., Phyto-mediated copper oxide nanoparticles for antibacterial, antioxidant and photocatalytic performances, Front Bioeng Biotechnol. 2022, 10, 820218.

[64] Soni A., Kaushal D., Kumar M., Sharma A., Maurya I. K., Kumar S., Synthesis, characterizations and antifungal activities of copper oxide and differentially doped copper oxide nanostructures. Materials Today, Proceedings, 2022.

[65] Bhat A. A., Thoker B. A., Wani A. K., Sheergojri G. A., Kaloo M. A., Bhatd B., Rizvie S., Syn¬thesis and characterization of copper oxide nanoparticles by coprecipitation method: electronic and antimicrobial properties, Chem. Sci. Eng. Res., 2021, 3, 25–9.

[66] Rehman S., Mumtaz A., Hasanain S., Size effects on the magnetic and optical properties of CuO NPs, J. Nanopart. Res., 2011, 13, 2497–507.

[67] Mobarak M. B., Hossain M. S., Chowdhury F., Ahmed S., Synthesis and characterization of CuO nanoparticles utilizing waste fish scale and exploitation of XRD peak profile analysis for approximating the structural parameters, Arab. J. Chem., 2022, 15, 104117.

[68] Mali S. C., Raj S., Trivedi R., Biosynthesis of copper oxide nanoparticles using Enicostemma axillare (Lam.) leaf extract., Biochem Biophys Rep., 2019, 20, 100699.

[69] Taghavi Fardood S., Moradnia F., Heidarzadeh S., Naghipour A., Green synthesis, characterization, photocatalytic and antibacterial activities of copper oxide nanoparticles of copper oxide nanoparticles, Nanochemistry Res., 2023, 8(2), 134–40.

[70] Mahmood R. I., Kadhim A. A., Ibraheem S., Albukhaty S., Mohammed-Salih H. S., Abbas R. H., Jabir M.S., Mohammed M. K., Nayef U. M., AlMalki F. A., Biosynthesis of copper oxide nanoparticles mediated Annona muricata as cytotoxic and apoptosis inducer factor in breast cancer cell lines, Sci. Rep., 2022, 12(1), 16165.

[71] Wongrakpanich A., Mudunkotuwa I. A., Geary S. M., Morris A. S., Mapuskar K. A., Spitz D. R., Grassian V. H., Salem A. K., Size-dependent cytotoxicity of cop¬per oxide nanoparticles in lung epithelial cells, Environ. Science: Nano., 2016, 3(2), 365–74.

[72] Tavakoli S., Kharaziha M., Ahmadi S., Green synthesis and morphology dependent antibacterial activity of copper oxide nanoparticles, J. Nanostruct., 2019, 9(1), 163–71.

[73] Gao P., Liu D., Facile synthesis of copper oxide nanostructures and their application in non-enzymatic hydrogen peroxide sensing, Sens Actuators B. 2015, 208:346–54.

[74] Mahmoud A. E. D., Al-Qahtani K. M., Alflaij S. O., Al-Qahtani S. F., Alsamhan F. A., Green copper oxide nanoparticles for lead, nickel, and cadmium removal from contaminated water. Sci Rep., 2021, 11(1), 12547.

[75] Anwaar S., Maqbool Q., Jabeen N., Nazar M., Abbas F., Nawaz B., Hussain T., Hussain S. Z., The effect of green synthesized CuO NPs on callogenesis and regeneration of Oryza sativa L. Front Plant Sci., 2016, 7, 1330.

[76] Padil V. V. T., Cernik M., Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application, Int. J. Nano¬med., 2013, 889–98.

[77] Khan M. F., Hameedullah M., Ansari A. H., Ahmad E., Lohani M., Khan R. H., Alam M. M., Khan W., Husain F. M., Ahmad I., Flower-shaped ZnO nanoparticles synthe¬sized by a novel approach at near-room temperatures with antibacterial and antifungal properties, Int. J. Nanomed., 2014, 853–64.

[78] Ma X., Zhou S., Xu X., Du Q., Copper-containing nanoparticles: mechanism of antimicrobial effect and application in dentistry-a narrative review. Front Surg. 2022, 9, 905892.

[79] Relhan A., Guleria S., Bhasin A., Mirza A., Zhou J. L., Biosynthesized copper oxide nanoparticles by Psidium guajava plants with antibacterial, antidiabetic, anti¬oxidant, and photocatalytic capacity. Biomass Convers Biorefinery. 2024, 1–18.

[80] Kaningini A. G., Motlhalamme T., More G. K., Mohale K. C., Maaza M., Antimicro¬bial, antioxidant, and cytotoxic properties of biosynthesized copper oxide nanoparticles (CuO-NPs) using Athrixia phylicoides D. C. Heliyon., 2023, 9(4).

[81] Peddi P., Ptsrk P. R., Rani N. U., Tulasi S. L., Green synthesis, characterization, antioxidant, antibacterial, and photocatalytic activity of Suaeda maritima (L.) Dumort aqueous extract-mediated copper oxide nanoparticles., J Genetic Eng Biotechnol., 2021, 19(1), 131.

How to cite this paper

Dr Surendra Kumar Sonwane, Haribati Maravi, Ishwar Singh Markam, Mitali Pardhi, Vandana Markam "Green Synthesis, Characterization and Evaluation of Antimicrobial Activity of Copper Oxide Nanoparticles Using Natural Extract of Ginger (Zingiber officinale)" Iconic Research And Engineering Journals Volume 10 Issue 1 2026 Page 2625-2638 https://doi.org/10.64388/IREV10I1-1720021
Dr Surendra Kumar Sonwane, Haribati Maravi, Ishwar Singh Markam, Mitali Pardhi, Vandana Markam "Green Synthesis, Characterization and Evaluation of Antimicrobial Activity of Copper Oxide Nanoparticles Using Natural Extract of Ginger (Zingiber officinale)" Iconic Research And Engineering Journals, vol. 10, no. 1, Jul. 2026, doi: https://doi.org/10.64388/IREV10I1-1720021
Dr Surendra Kumar Sonwane, Haribati Maravi, Ishwar Singh Markam, Mitali Pardhi, Vandana Markam (2026). Green Synthesis, Characterization and Evaluation of Antimicrobial Activity of Copper Oxide Nanoparticles Using Natural Extract of Ginger (Zingiber officinale). Iconic Research And Engineering Journals, 10(1). doi: https://doi.org/10.64388/IREV10I1-1720021
Dr Surendra Kumar Sonwane, Haribati Maravi, Ishwar Singh Markam, Mitali Pardhi, Vandana Markam "Green Synthesis, Characterization and Evaluation of Antimicrobial Activity of Copper Oxide Nanoparticles Using Natural Extract of Ginger (Zingiber officinale)" Iconic Research And Engineering Journals, vol. 10, no. 1, Jul. 2026. Crossref, https://doi.org/10.64388/IREV10I1-1720021
@article{1720021,
      author = {Dr Surendra Kumar Sonwane, Haribati Maravi, Ishwar Singh Markam, Mitali Pardhi, Vandana Markam },
      title = {Green Synthesis, Characterization and Evaluation of Antimicrobial Activity of Copper Oxide Nanoparticles Using Natural Extract of Ginger (Zingiber officinale)},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {10},
      number = {1},
      pages = {2625-2638},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1720021.pdf},
      abstract = {The green synthesis of CuO nanoparticles using plant extracts, specifically ginger (Zingiber officinale) was studied for its affordability and eco-friendly traits. The CuO NPs were analyzed via Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (FESEM-EDX) and TEM.  Their antimicrobial effectiveness was evaluated against two bacterial strains Escherichia coli ( E. coli) and Staphylococcus aureus ( S. aureus) and two fungal species Fusarium oxysporum (F. oxysporum) and Fusarium graminearum (F.graminearum) using the disk diffusion technique. The biosynthesized CuO nanoparticles were evaluated alongside chemically synthesized CuO nanoparticles to assess how the synthesis method influences their structural, optical, morphological properties, as well as their antibacterial and antifungal efficacy. The topological characteristics including dimensions, shapes, aggregation behavior, and basic structural arrangements of green-synthesized CuO nanoparticles are critical determinants of their biological activity. Investigations serve as valuable methods for studying these properties. TEM analysis confirmed that the biosynthesized CuO nanoparticles have a spherical shape. Particle size distribution analysis showed a size range of 4 to 28 nm, with an average diameter of 16.2 nm.},
      keywords = {Nanocomposites, Green Synthesis, Natural, Extracts Ginger, Antibacterial and Antifungal Activity.},
      month = {July},
      doi = {https://doi.org/10.64388/IREV10I1-1720021}
  }