International Peer-Reviewed Journal•Open Access•ISSN 2456-8880
[email protected]•+91-7433024337

Home / Current Issue / Paper 1722600

1722600 Vol 10 · Issue 2 Download Paper

Antibacterial Activities of Water Melon (Citrillus Lanatu) Seed Extracts Against Some Selected Bacteria

Abdulwahab Musa F. A. Abadam Yakaka Mohammed Alkali Mohammed Buba

Subject area: Science,Engineering and Technology  ·  Area of research: Science

DOI: 10.64388/ire1722600

Abstract

Plants are reservoirs of different kinds of phytochemicals used for treatment of infections and other diseases. This study was aimed at evaluating the antibacterial activities of Citrullus lanatus (watermelon) seeds against some selected bacteria. Ethanol was used to obtain the extracts and they were analyzed for the presence of carbohydrates, alkaloids, glycosides, tannins and saponins. Out of the phytochemicals screened, only saponins were not detected in both aqueous and ethanolic extract. Staphylococcus aureus, Escherichia coli, bacillus spp. and Proteus spp. were selected for antibiotic susceptibility testing. Antibacterial activity revealed that S. aureus, E. coli, and proteus spp. were most Susceptible to the extracts at concentrations ranging from 50mg/ml to 25mg/ml. Proteus spp. was observed to be resistant especially to the ethanolic extract. Largest zone of inhibition (25mm) was produced against Staphylococcus aureus using aqueous extract at 50mg/ml. Higher concentrations were generally effective than the lower concentration whereas, ciprofloxacin (control) exert better activity than either of the extracts. The findings of this study showed that seeds of C. lanatus contained bioactive compounds with potent antibacterial activity and thus could be used as herbal preparations just as the other parts of the plant.

Keywords

antibacterial, phytochemical, proximate analysis, solvent, Watermelon

References

[1] Adewuyi A, Oderinde R, Ademisoye A (2013). Antibacterial activities of nonionic and anionic surfactants from Citrullus lanatus seed oil. Jundishapur Journal of Microbiology, 6(3), 205–208.

[2] Adoum OA, Akinniyi JA, Omar T (1997). The effect of geographical location on the antimicrobial activities and trace element concentrations in the root of Calotropis procera (Ait.) R. Br. Annals of Borno, 13, 199–207.

[3] Adunola AT, Chidimma AL, Olatunde DS, Peter OA (2015). Antibacterial activity of watermelon (Citrullus lanatus) seed against selected microorganisms. African Journal of Biotechnology, 14, 1224–1229.

[4] Ajayi IA, Ajibade O, Oderinde RA (2011). Preliminary phytochemical analysis of some plant seeds. Research Journal of Chemical Sciences, 1, 58–62.

[5] Braide W, Odiong IJ, Oranusi S (2012). Phytochemical and antibacterial properties of the seed of watermelon (Citrullus lanatus). Prime Journal of Microbiology Research, 2(3), 99–104.

[6] Elujoba AA, Odeleye OM, Ogunyemi CM (2005). Traditional medicine development for medical and dental primary health care delivery system in Africa. African Journal of Traditional, Complementary and Alternative Medicines, 2, 46–61.

[7] Evans WC (2002). General methods associated with the phytochemical investigation of herbal products. In: Trease and Evans pharmacognosy, 15th ed., 137–148. Saunders (Elsevier), New Delhi.

[8] Godwin OO, Williams A, Andrew NA, Aoyebi B, Ezeh PA, Udosen (2015). [Reference incomplete in the manuscript.]

[9] Handa S (2008). Extraction technologies for medicinal and aromatic plants. In: Handa S, Khanuja S, Longo G, Rakesh D (eds.), Extraction technologies for medicinal and aromatic plants, 22. United Nations Industrial Development Organization and the International Centre for Science and High Technology, Trieste.

[10] Hassan LEA, Sirat HM, Yagi SMA, Koko WS, Abdelwahab SI (2011). In vitro antimicrobial activities of chloroformic, hexane and ethanolic extracts of Citrullus lanatus var. citroides (wild melon). Journal of Medicinal Plants Research, 5(8), 1338–1344.

[11] Lakshmi AJ, Kaul P (2011). Nutritional potential, bioaccessibility of minerals and functionality of watermelon (Citrullus vulgaris) seeds. LWT – Food Science and Technology, 44, 1821–1826.

[12] Lalitha MK (2014). Manual on antimicrobial susceptibility testing. Indian Association of Medical Microbiologists, Tamil Nadu.

[13] Meena MC, Patni V (2008). Isolation and identification of flavonoid “quercetin” from Citrullus colocynthis (Linn.) Schrad. Asian Journal of Experimental Sciences, 22(1), 137–142.

[14] Njoroge GN, Gemmill B, Bussmann R, Newton LE, Ngumi VW (2007). Pollination ecology of Citrullus lanatus at Yatta, Kenya. International Journal of Tropical Insect Science, 24, 73–77.

[15] Nwankwo IU, Onwuakor CE, Nwosu VC (2014). Phytochemical analysis and antibacterial activities of Citrullus lanatus seed against some pathogenic microorganisms. Global Journal of Medical Research, 14, 17–22.

[16] Okorondu SI, Sokeri TG, Akujobi CO, Braide W (2010). Phytochemical and antimicrobial properties of Musa paradisiaca stalk plant. International Journal of Biological Sciences, 2, 128–132.

[17] Okunrobo OL, Uwaya OJ, Imafidon EK, Osarumwense OP, Omorodion EJ (2012). Quantitative determination, metal analysis and antiulcer evaluation of methanol seeds extract of Citrullus lanatus Thunb (Cucurbitaceae) in rats. Asian Pacific Journal of Tropical Disease, S804–S805.

[18] Omigie IO, Agoreyo FO (2014). Effects of watermelon (Citrullus lanatus) seed on blood glucose and electrolyte parameters in diabetic Wistar rats. Journal of Applied Sciences and Environmental Management, 18(2), 231–233.

[19] Oseni OA, Okoye VI (2013). Studies of phytochemical and antioxidant properties of the fruit of watermelon (Citrullus lanatus) (Thunb.). Journal of Pharmaceutical and Biomedical Sciences, 27(27), 508–514.

[20] Oyeleke GO, Olagunju EO, Ojo A (2012). Functional and physicochemical properties of watermelon (Citrullus lanatus) seed and seed oil. IOSR Journal of Applied Chemistry, 2(2), 29–31.

[21] Rahman B (2014). Phytochemical investigation of Citrullus lanatus (watermelon) rind [Thesis]. Department of Pharmacy, East West University, Bangladesh.

[22] Schulz V, Hänsel R, Tyler VE (2001). Rational phytotherapy: A physician’s guide to herbal medicine, 4th ed. Springer-Verlag, Berlin.

[23] Sen IJ (2015). An assessment of the phytochemicals and antibacterial activity of seed extract of Citrullus lanatus (watermelon). International Journal of Research & Review, 148–156.

[24] Soetan KO, Oyekunle MA, Aiyelaagbe OO, Fafunso MA (2006). Evaluation of the antimicrobial activity of saponins extract of Sorghum bicolor L. Moench. African Journal of Biotechnology, 5(23), 2405–2407.

[25] Thirunavukkarasu P, Ramanathan T, Ravichandran N, Ramkumar L (2010). Screening of antimicrobial effect in watermelon (Citrullus sp.). Journal of Biological Sciences, 10, 682–685.

[26] Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H (2011). Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia, 1, 98–106.

[27] Trease GE, Evans WC (1989). A textbook of pharmacognosy, 13th ed., 176–180. Baillière Tindall and Cassell, London.

[28] Varghese S, Narmadha R, Gomathi D, Kalaiselvi M, Devaki K (2013). Phytochemical screening and HPTLC finger printing analysis of Citrullus lanatus (Thunb.) seed. Journal of Acute Disease, 2(2), 122–126.

[29] Wangensteen H, Samuelsen AB, Malterud KE (2004). Antioxidant activity in extracts from coriander. Food Chemistry, 88, 293–297.

[30] Wehner TC (2008). Watermelon. In: Vegetables I: Asteraceae, Brassicaceae, Chenopodiaceae and Cucurbitaceae, 381–418. Springer, New York.

[31] World Health Organization (WHO) (2005). National policy on traditional medicine and regulation of herbal medicines: Report of a WHO global survey. WHO, Geneva.

[32] Yuan G, Wahlqvist ML, He G, Yang M, Li D (2006). Natural products and anti-inflammatory activity. Asia Pacific Journal of Clinical Nutrition, 15, 143–152.

How to cite this paper

Abdulwahab Musa, F. A. Abadam, Yakaka Mohammed Alkali, Mohammed Buba "Antibacterial Activities of Water Melon (Citrillus Lanatu) Seed Extracts Against Some Selected Bacteria" Iconic Research And Engineering Journals Volume 10 Issue 2 2026 Page 3826-3832 https://doi.org/10.64388/ire1722600
Abdulwahab Musa, F. A. Abadam, Yakaka Mohammed Alkali, Mohammed Buba "Antibacterial Activities of Water Melon (Citrillus Lanatu) Seed Extracts Against Some Selected Bacteria" Iconic Research And Engineering Journals, vol. 10, no. 2, Aug. 2026, doi: https://doi.org/10.64388/ire1722600
Abdulwahab Musa, F. A. Abadam, Yakaka Mohammed Alkali, Mohammed Buba (2026). Antibacterial Activities of Water Melon (Citrillus Lanatu) Seed Extracts Against Some Selected Bacteria. Iconic Research And Engineering Journals, 10(2). doi: https://doi.org/10.64388/ire1722600
Abdulwahab Musa, F. A. Abadam, Yakaka Mohammed Alkali, Mohammed Buba "Antibacterial Activities of Water Melon (Citrillus Lanatu) Seed Extracts Against Some Selected Bacteria" Iconic Research And Engineering Journals, vol. 10, no. 2, Aug. 2026. Crossref, https://doi.org/10.64388/ire1722600
@article{1722600,
      author = {Abdulwahab Musa, F. A. Abadam, Yakaka Mohammed Alkali, Mohammed Buba},
      title = {Antibacterial Activities of Water Melon (Citrillus Lanatu) Seed Extracts Against Some Selected Bacteria},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {10},
      number = {2},
      pages = {3826-3832},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1722600.pdf},
      abstract = {Plants are reservoirs of different kinds of phytochemicals used for treatment of infections and other diseases. This study was aimed at evaluating the antibacterial activities of Citrullus lanatus (watermelon) seeds against some selected bacteria. Ethanol was used to obtain the extracts and they were analyzed for the presence of carbohydrates, alkaloids, glycosides, tannins and saponins. Out of the phytochemicals screened, only saponins were not detected in both aqueous and ethanolic extract. Staphylococcus aureus, Escherichia coli, bacillus spp. and Proteus spp. were selected for antibiotic susceptibility testing. Antibacterial activity revealed that S. aureus, E. coli, and proteus spp. were most Susceptible to the extracts at concentrations ranging from 50mg/ml to 25mg/ml. Proteus spp. was observed to be resistant especially to the ethanolic extract. Largest zone of inhibition (25mm) was produced against Staphylococcus aureus using aqueous extract at 50mg/ml. Higher concentrations were generally effective than the lower concentration whereas, ciprofloxacin (control) exert better activity than either of the extracts. The findings of this study showed that seeds of C. lanatus contained bioactive compounds with potent antibacterial activity and thus could be used as herbal preparations just as the other parts of the plant.},
      keywords = {antibacterial, phytochemical, proximate analysis, solvent, Watermelon},
      month = {August},
      doi = {https://doi.org/10.64388/ire1722600}
  }