Protein Quality Indices and Growth Performance of Albino Rats Fed Sprouted Soybean, African Yam Bean and Red Kidney Bean Diets

References

[1] Adebayo, J. O., & Ologhobo, A. D. (2020). Nutritional evaluation of mixed plant protein diets on growth performance in rats. Journal of Food Biochemistry, 44(5), e13123.

[2] Adebayo, S. E., Kareem, S. O., & Adegboye, A. R. (2020). Nutritional significance of legumes in human and animal diets. Journal of Food Biochemistry, 44(3), e13118.

[3] Adebayo, S. E., Kareem, S. O., & Adegboye, A. R. (2022). Legume proteins as functional and health-promoting ingredients in food systems. Journal of Food Biochemistry, 46(2), e13905.

[4] Adebo, O. A., Kayitesi, E., Adebiyi, J. A., & Njobeh, P. B. (2022). Fermentation and germination as sustainable processing technologies for improving nutritional quality of legumes: A review. Food Research International, 152, 110879.

[5] Alagboso, S. I., Ibeh, C. A., & Egwim, E. C. (2017). Growth performance and feed efficiency in rats fed legume-based diets. Journal of Animal Science and Technology, 10(2), 45–53.

[6] Aremu, M. O., Ibrahim, H., & Bamidele, T. O. (2022). Nutritional composition and food applications of African yam bean (Sphenostylis stenocarpa): A review. Legume Science, 4(2), e143.

[7] Boukid, F., Rosell, C. M., & Castellari, M. (2023). Grain legumes as sustainable protein sources for future foods: Nutritional quality, health benefits and processing opportunities. Foods, 12(8), 1604.

[8] Gan, R. Y., Lui, W. Y., Wu, K., Chan, C. L., Dai, S. H., Sui, Z. Q., & Corke, H. (2021). Bioactive compounds and bioactivities of germinated edible seeds and sprouts: An updated review. Trends in Food Science & Technology, 109, 619–632.

[9] Igwilo, I. O., Okoro, G. A., & Nwachukwu, A. O. (2011). Formulation of diets from red kidney beans, soybean, and African yam bean flour blends for animal feed production. Journal of Agricultural Sciences, 9(4), 56–64.

[10] National Academies of Sciences, Engineering, and Medicine. (2023). Nutrient requirements of laboratory animals. National Academies Press.

[11] National Research Council. (2011). Guide for the care and use of laboratory animals (8th ed.). National Academies Press.

[12] Nwosu, J. N., Onuegbu, N. C., & Ojimelukwe, P. C. (2020). Effect of soaking and sprouting on anti-nutritional factors in legumes. African Journal of Food Science, 14(3), 65–73.

[13] Oluwole, F. A., Obasa, S. O., & Afolabi, T. A. (2020). Processing of soybeans for flour production. Journal of Food Engineering and Technology, 9(2), 55–60.

[14] Onyeka, U. E. (2008). Food and nutrition (2nd ed.). Charismatic Forum Publishers.

[15] Rutherfurd, S. M., Fanning, A. C., Miller, B. J., & Moughan, P. J. (2022). Protein digestibility-corrected amino acid scores and digestible indispensable amino acid scores: Current perspectives. Animal Frontiers, 12(4), 52–59.

[16] Samtiya, M., Aluko, R. E., Dhewa, T., & Moreno-Rojas, J. M. (2020). Potential health benefits of plant food-derived bioactive components: An overview. Foods, 9(6), 839.

[17] Tukamuhabwa, P. A., Mukiibi, J., & Mbarara, M. (2016). Modified method for preparation of red kidney bean flour. Journal of Food Science and Technology, 53(3), 963–970.

@article{1722638,
      author = {Ojeh Grace Okechukwu , Okorie Stanislaus Udeze},
      title = {Protein Quality Indices and Growth Performance of Albino Rats Fed Sprouted Soybean, African Yam Bean and Red Kidney Bean Diets},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {10},
      number = {2},
      pages = {3372-3378},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1722638.pdf},
      abstract = {The search for sustainable plant-based protein sources has intensified due to the increasing prevalence of protein-energy malnutrition and the rising cost of animal proteins. Sprouting is an effective bioprocessing technique known to improve the nutritional quality and digestibility of legumes by reducing anti-nutritional factors and enhancing nutrient bioavailability. This study evaluated the influence of sprouted soybean (Glycine max), African yam bean (Sphenostylis stenocarpa), and red kidney bean (Phaseolus vulgaris) protein diets on protein quality indices and growth performance in albino rats. Soybean, African yam bean, and red kidney bean seeds were sprouted for 48 h, oven-dried at 55 °C, milled into flour, and blended in nine different proportions. Experimental diets were formulated to contain 10% crude protein and fed to groups of albino rats for 21 days, while commercial rat chow served as the control. Growth performance was evaluated using feed intake, body weight gain, feed efficiency ratio, and protein efficiency ratio. Protein quality was assessed using apparent protein digestibility, nitrogen balance, biological value, net protein utilization, and net protein ratio following standard nitrogen-balance procedures. Data were analyzed using one-way analysis of variance, and treatment means were separated using Duncan’s multiple range test at p < 0.05. Significant (p < 0.05) differences were observed among the dietary treatments for all growth-performance and protein-quality parameters. Rats fed the commercial control diet recorded the highest growth performance and protein-utilization indices. Among the experimental diets, the blend containing 50% sprouted soybean and 50% sprouted red kidney bean (LB5) produced superior values for several growth and protein-quality indices. The improved nutritional response observed in mixed-legume diets may be associated with complementary amino-acid profiles and the beneficial effects of sprouting on anti-nutritional factors and protein digestibility. The findings indicate that selected sprouted soybean–African yam bean–red kidney bean blends have potential as affordable plant-based protein ingredients for food-product development.},
      keywords = {African yam bean; albino rats; growth performance; legume protein; protein quality; red kidney bean; soybean; sprouting.},
      month = {August},
      doi = {https://doi.org/10.64388/IREV10I2-1722638}
  }