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Invitro Study of the Antioxidant and Anti-Inflammatory Potential of Rauvolfia Vomitoria Afzel Leaf Crude Extract
Subject area: Biological & Medical Sciences · Area of research: Toxicology and Drug Metabolism
DOI: https://doi.org/10.64388/IREV10I3-1722821
Abstract
Oxidative stress is a physiological term that describes an imbalance between reactive oxygen species (ROS) generation and the body’s ability to detoxify them or repair the resulting damage. Scientific validation of traditional medicinal plants is essential for their integration into modern healthcare systems and pharmaceutical developments. This study aimed to fill the existing knowledge gap by examining the antioxidant potential of R. vomitoria crude ex3tract using multiple assay methods and comparing the results with standard ascorbic acid. Rauvofia vomitoria leaves were powdered and soaked in 100% methanol for 48 hours at room temperature with occasional agitations.. The extract demonstrated notable inhibitions of protein denaturation, trypsin activity, and DNA oxidation to fight inflammation. The study provided compelling evidence of anti-inflammatory potential of Rauvolfia vomitoria.
Keywords
inflammation, ascorbic acid, trypsin, denaturation, inhibition, standard, pharmaceutical, medicinal
References
[1] Adebayo, A. A., Ademosun, A. O., and Oboh, G. (2023). Chemical composition, antioxidant, and enzyme inhibitory properties of Rauvolfia vomitoria extracts. Journal of Ethnopharmacology, 310, 116295.
[2] Adedapo, A. A., Sofidiya, M. O., and Afolayan, A. J. (2008). Antioxidant and antimicrobial properties of the methanol extract of the leaves and stems of Rauvolfia vomitoria and Rauvolfia caffra. African Journal of Traditional, Complementary and Alternative Medicines, 5(4), 355–361.
[3] Aggarwal, B. B., Sundaram, C., Malani, N., and Ichikawa, H. (2007). Curcumin: The Indian solid gold. Advances in Experimental Medicine and Biology, 595, 1–75. Crossref
[4] Akeghware, O. A., Oboh, G., and Ademosun, A. O. (2023). Comparative antioxidant activity of aqueous and ethanolic leaf extracts of Rauvolfia vomitoria. Phytomedicine Plus, 3(1), 100295.
[5] Akinmoladun, F. O., Akinrinlola, B. L., and Farombi, E. O. (2010). Antioxidant activity of African medicinal plants. Journal of Medicinal Plants Research, 4(11), 1109–1115.
[6] Akinmoladun, F. O., Akinrinlola, B. L., Komolafe, T. O., Farombi, E. O., and Olaleye, T. M. (2010). Antioxidant and phytochemical properties of Rauvolfia vomitoria (Apocynaceae). African Journal of Biotechnology, 9(3), 326–330.
[7] Akinyele, B. O., and Amoo, I. A. (2014). Nutritional and phytochemical evaluation of watermelon seed (Citrullus lanatus) from Nigeria. Journal of Scientific Research & Reports, 3(12), 1540–1548.
[8] Akpambang, V. O. E., Amoo, I. A., and Izuagie, A. A. (2008). Comparative compositional analysis on two varieties of melon (Citrullus lanatus) seeds from Nigeria. Research Journal of Agriculture and Biological Sciences, 4(5), 639–642.
[9] Aliyu, A. B., Achika, J. I., Adewuyi, J. A., Gangas, P., Ibrahim, H., and Oyewale, A. O. (2019). Antioxidants from Nigerian Medicinal Plants: What Are the Evidence? In Lipid Peroxidation Research. IntechOpen. Crossref
[10] Ayala, A., Muñoz, M. F., and Argüelles, S. (2014). Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity, 2014, 360438. Crossref
[11] Ayoub, Z., and Mehta, A. (2018). Medicinal plants as potential source of antioxidant agents: A review. International Journal of Pharmacy and Pharmaceutical Sciences, 11(6), 1–10. Crossref
[12] Benzie, I. F. F., and Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70–76. Crossref
[13] Bjelakovic, G., Nikolova, D., Gluud, L. L., Simonetti, R. G., and Gluud, C. (2007). Mortality in randomized trials of antioxidant supplements for primary and secondary prevention: Systematic review and meta- analysis. JAMA, 297(8), 842–857. Crossref
[14] Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199–1200. Crossref
[15] Brand-Williams, W., Cuvelier, M. E., and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25–30. Crossref
[16] Brigelius-Flohé, R., and Maiorino, M. (2013). Glutathione peroxidases. Biochimica et Biophysica Acta (BBA) - General Subjects, 1830(5), 3289–3303. Crossref
[17] Brigelius-Flohé, R., and Traber, M. G. (1999). Vitamin E: Function and metabolism. FASEB Journal, 13(10), 1145–1155. Crossref
[18] Brownlee, M. (2001). Biochemistry and molecular cell biology of diabetic complications. Nature, 414(6865), 813–820. Crossref
[19] Cabrera, C., Artacho, R., and Giménez, R. (2006). Beneficial effects of green tea—A review. Journal of the American College of Nutrition, 25(2), 79–99. Crossref
[20] Carr, A., and Frei, B. (1999). Toward a new recommended dietary allowance for vitamin C based on antioxidant and health effects in humans. American Journal of Clinical Nutrition, 69(6), 1086–1107. Crossref
[21] Chance, B., Greenstein, D. S., and Roughton, F. J. W. (1979). The kinetics of catalase action. Journal of Biological Chemistry, 244(2), 379–389.
[22] Chandra, S., Chatterjee, P., Dey, P., and Bhattacharya, S. (2012). Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine, 2(1), S178– S180. Crossref
[23] Chelikani, P., Fita, I., and Loewen, P. C. (2004). Diversity of structures and properties among catalases. Cellular and Molecular Life Sciences, 61(2), 192–208. Crossref
[24] Cooke, M. S., Evans, M. D., Dizdaroglu, M., and Lunec, J. (2003). Oxidative DNA damage: Mechanisms, mutation, and disease. FASEB Journal, 17(10), 1195–1214. Crossref
[25] Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582. Crossref
[26] Flohé, L., and Günzler, W. A. (1984). Glutathione peroxidase. In Methods in Enzymology (Vol. 105, pp. 114–121). Academic Press.
[27] Halliwell, B., and Gutteridge, J. M. C. (2015). Free radicals in biology and medicine (5th ed.). Oxford University Press.
[28] Kahl, R., and Kappus, H. (1993). Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E. Zeitschrift für Lebensmittel- Untersuchung und -Forschung, 196(4), 329– 338.
[29] Krinsky, N. I., and Johnson, E. J. (2005). Carotenoid actions and their relation to health and disease. Molecular Aspects of Medicine, 26(6), 459–516. Crossref
[30] Lin, M. T., and Beal, M. F. (2006). Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature, 443(7113), 787–795.
[31] Liu, R. H. (2004). Potential synergy of phytochemicals in cancer prevention: Mechanism of action. The Journal of Nutrition, 134(12), 3479S–3485S. Crossref
[32] Lobo, V., Patil, A., Phatak, A., and Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118–126. Crossref
[33] Ma, Q. (2013). Role of Nrf2 in oxidative stress and toxicity. Annual Review of Pharmacology and Toxicology, 53, 401–426. Crossref
[34] Madamanchi, N. R., Vendrov, A., and Runge, M. S. (2005). Oxidative stress and vascular disease. Arteriosclerosis, Thrombosis, and Vascular Biology, 25(1), 29–38. Crossref
[35] McCord, J. M., and Fridovich, I. (1969). Superoxide dismutase: An enzymic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry, 244(22), 6049–6055. Crossref
[36] Middleton, E., Kandaswami, C., and Theoharides, T. C. (2000). The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacological Reviews, 52(4), 673–751.
[37] Murphy, M. P. (2009). How mitochondria produce reactive oxygen species. Biochemical Journal, 417(1), 1–13. Crossref
[38] Nwozo, O. S., Effiong, E. M., Ajab, P. M., and Awuchi, C. G. (2023). Antioxidant, phytochemical, and therapeutic properties of medicinal plants: A review. International Journal of Food Properties, 26(1), 359–388. Crossref
[39] Odugbemi, T. (2008). Outlines and pictures of medicinal plants from Nigeria. University of Lagos Press.
[40] Ogbole, O. O., Segun, P. A., and Ajaiyeoba, E. O. (2018). Antioxidant and anti- inflammatory activities of Rauvolfia vomitoria (Apocynaceae) root bark extract and fractions. African Journal of Traditional, Complementary and Alternative Medicines, 15(1), 1–8.
[41] Okoli, R. I., Aigbe, O., Ohaju-Obodo, J. O., and Mensah, J. K. (2007). Medicinal herbs used for managing some common ailments among Esan people of Edo State, Nigeria. Pakistan Journal of Nutrition, 6(5), 490–496.
[42] Okolie, N. P., Israel, E. E. J., and Falodun, A. (2011). In-vitro evaluation of antioxidant potential of Rauvolfia vomitoria root extract and its inhibitory effect on lipid peroxidation. Pharmaceutical Chemistry Journal, 45(7), 476–480.
[43] Oliver-Bever, B. (1986). Medicinal plants in tropical West Africa. Cambridge University Press.
[44] Pham-Huy, L. A., He, H., and Pham-Huy, C. (2008). Free radicals, antioxidants in disease and health. International Journal of Biomedical Science, 4(2), 89–96.
[45] Pietta, P. G. (2000). Flavonoids as antioxidants. Journal of Natural Products, 63(7), 1035–1042. Crossref
[46] Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., et al. (2017). Oxidative stress: Harms and benefits for human health. Oxidative Medicine and Cellular Longevity, 2017, 8416763. Crossref
[47] Prior, R. L., Wu, X., and Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53(10), 4290–4302.
[48] Reuter, S., Gupta, S. C., Chaturvedi, M. M., and Aggarwal, B. B. (2010). Oxidative stress, inflammation, and cancer: How are they linked? Free Radical Biology and Medicine, 49(11).
[49] Rice-Evans, C. A., Miller, N. J., and Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science, 2(4), 152–159. Crossref
[50] Serrano, J., Puupponen-Pimiä, R., Dauer, A., Aura, A. M., and Saura-Calixto, F. (2009). Tannins: Current knowledge of food sources, intake, bioavailability and biological effects. Molecular Nutrition and Food Research, 53(S2), S310–S329. Crossref
[51] Shi, J., Arunasalam, K., Yeung, D., Kakuda, Y., Mittal, G., and Jiang, Y. (2004). Saponins from edible legumes: Chemistry, processing, and health benefits. Journal of Medicinal Food, 7(1), 67–78. Crossref
[52] Sonibare, M. A., and Akpan, G. U. (2017). In vitro callus induction and antioxidant activity of Rauvolfia vomitoria Afzel. Nigerian Journal of Pharmaceutical Research, 12(2), 163–170.
[53] Sultana, N., Saini, P. K., Kiran, K., Rout, S., and Kanaka, S. (2023). Exploring the Antioxidant Potential of Medicinal Plant Species: A Comprehensive Review. ResearchFloor.org.
[54] Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T. D., Mazur, M., and Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human
[55] Wu, G., Fang, Y. Z., Yang, S., Lu, T., and Li, H. (2004). Glutathione metabolism and its implications for health. Journal of Nutrition, 134(3), 489–492. Crossref
[56] Zelko, I. N., Mariani, T. J., and Folz, R. J. (2002). Superoxide dismutase multigene family: A comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radical Biology and Medicine, 33(3), 337–3. Crossref
How to cite this paper
@article{1722821,
author = {Arowolo A. S., Bamigboye Adeola, Akinyemi A. O.},
title = {Invitro Study of the Antioxidant and Anti-Inflammatory Potential of Rauvolfia Vomitoria Afzel Leaf Crude Extract},
journal = {Iconic Research And Engineering Journals},
year = {2026},
volume = {10},
number = {3},
pages = {1547-1556},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1722821.pdf},
abstract = {Oxidative stress is a physiological term that describes an imbalance between reactive oxygen species (ROS) generation and the body’s ability to detoxify them or repair the resulting damage. Scientific validation of traditional medicinal plants is essential for their integration into modern healthcare systems and pharmaceutical developments. This study aimed to fill the existing knowledge gap by examining the antioxidant potential of R. vomitoria crude ex3tract using multiple assay methods and comparing the results with standard ascorbic acid. Rauvofia vomitoria leaves were powdered and soaked in 100% methanol for 48 hours at room temperature with occasional agitations.. The extract demonstrated notable inhibitions of protein denaturation, trypsin activity, and DNA oxidation to fight inflammation. The study provided compelling evidence of anti-inflammatory potential of Rauvolfia vomitoria.},
keywords = {inflammation, ascorbic acid, trypsin, denaturation, inhibition, standard, pharmaceutical, medicinal},
month = {September},
doi = {https://doi.org/10.64388/IREV10I3-1722821}
}