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Effect of Physicochemical Parameters On Antioxidant Activity of Hydrolysed Tilapia Protein
Subject area: Science,Engineering and Technology · Area of research: Marine Biology, Fisheries and Aquaculture
Abstract
Marine-derived proteins and their hydrolysed peptide fractions exhibit high bioactivity due to their unique amino acid composition distinct from terrestrial protein sources. This study examined the effect of enzyme concentration, temperature, and hydrolysis time on the antioxidant activity of papain-hydrolysed tilapia protein. Protein hydrolysates were prepared by enzymatic hydrolysis using papain and purified by gel filtration chromatography, following standard analytical protocols. Response surface methodology (RSM) based on a Box-Behnken design was used to model the influence of enzyme concentration, temperature, and hydrolysis time on antioxidant activity (DPPH radical scavenging), across 15 experimental runs. DPPH scavenging activity ranged from 51.86% to 92.17% across the design space. Maximum antioxidant activity occurred at an enzyme concentration of 4%, temperature of 60 °C, and hydrolysis time of 5 h. The temperature × hydrolysis time interaction (AC) was non-significant (p > 0.05), indicating that the two variables exert independent main effects rather than acting synergistically. The model followed an additive response topology; whereby peak antioxidant activity is predicted when temperature and hydrolysis time are set concurrently at their individually optimal values. These findings demonstrate that enzyme concentration, temperature, and hydrolysis time independently govern the antioxidant activity of papain-hydrolysed tilapia protein, providing a basis for optimising hydrolysis conditions to maximise bioactive peptide yield.
Keywords
Tilapia protein, physicochemical parameters, antioxidant
References
[1] Ameen, F., AlNadhari, S., and Al-Homaidan, A.A. (2021). Marine microorganisms as an untapped source of bioactive compounds. Saudi J Biol Sci. 28(1):224-231. ScienceDirect
[2] Coppola, D., Oliviero, M., and Vitale, G.A. (2022). Marine collagen from alternative and sustainable sources: extraction, processing and applications. Mar Drugs. 18(4):214.
[3] Giordano, D., Costantini, M., and Coppola, D. (2018). Biotechnological applications of bioactive peptides from marine sources. Adv Microb Physiol. 73, 171-220.
[4] Hu, W., Wang, X.M., and Chi, Y.M. (2022). Bioactive peptides from skipjack Tuna cardiac arterial bulbs: preparation, identification, antioxidant activity, and stability against thermal, pH, and simulated gastrointestinal digestion treatments. Mar Drugs. 20(10),626-635.
[5] Hu, Y.D., Xi, Q.H., Kong, J., Zhao, Y,Q., Chi, C.F., and Wang, B. (2023). Angiotensin-I-converting enzyme (ace)-inhibitory peptides from the collagens of monkfish (Lophius litulon) swim bladders: isolation, characterization, molecular docking analysis and activity evaluation. Mar Drugs. 21(10), 516-526.
[6] Khan, S., and Malik, A. (2021). Exploring the diversity of marine microbiome in response to changes in the environment. Microbiomes Glob Clim Chang. 5(3), 81-92. Springer
[7] Pratama, I.S., Putra, Y., Pangestuti, R., Kim, S.K., and Siahaan, E.A. (2022). Bioactive peptides-derived from marine by-products: development, health benefits and potential application in biomedicine. Fish Aquat Sci. 25(7), 357-379. Fisheries and Aquatic Sciences
[8] Srinivasan, R., Kannappan, A., Shi, C., and Lin, X. (2021). Marine bacterial secondary metabolites: a treasure house for structurally unique and effective antimicrobial compounds. Mar Drugs. 19(10), 530-536. MDPI
[9] Suo, S.K., Zhao, Y.Q., Wang, Y.M., Pan, X.Y., Chi, C.F., and Wang, B. (2022). Seventeen novel angiotensin converting enzyme (ACE) inhibitory peptides from the protein hydrolysate of Mytilus edulis: isolation, identification, molecular docking study, and protective function on HUVECs. Food Funct. 13(14), 7831-7846. Royal Society of Chemistry
[10] Välimaa, A.L., Mäkinen, S., and Mattila, P. (2019). Fish and fish side streams are valuable sources of high-value components. Food Qual Saf. 3(4), 209-226.
[11] Wu, M.F., Xi, Q.H., and Sheng, Y. (2023). Antioxidant peptides from monkfish swim bladders: ameliorating NAFLD in vitro by suppressing lipid accumulation and oxidative stress via regulating AMPK/Nrf2 pathway. Mar Drugs. 21(6), 360-368.
How to cite this paper
@article{1723440,
author = {Abdulsalam, I., Ishola, N.O., Odedere, A. O.; Dickson Musa, A., Abadi, N.; Abdulazeez, M., Hashimu, A.; Yusuf Bin, M.},
title = {Effect of Physicochemical Parameters On Antioxidant Activity of Hydrolysed Tilapia Protein},
journal = {Iconic Research And Engineering Journals},
year = {2026},
volume = {10},
number = {3},
pages = {4013-4018},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1723440.pdf},
abstract = {Marine-derived proteins and their hydrolysed peptide fractions exhibit high bioactivity due to their unique amino acid composition distinct from terrestrial protein sources. This study examined the effect of enzyme concentration, temperature, and hydrolysis time on the antioxidant activity of papain-hydrolysed tilapia protein. Protein hydrolysates were prepared by enzymatic hydrolysis using papain and purified by gel filtration chromatography, following standard analytical protocols. Response surface methodology (RSM) based on a Box-Behnken design was used to model the influence of enzyme concentration, temperature, and hydrolysis time on antioxidant activity (DPPH radical scavenging), across 15 experimental runs. DPPH scavenging activity ranged from 51.86% to 92.17% across the design space. Maximum antioxidant activity occurred at an enzyme concentration of 4%, temperature of 60 °C, and hydrolysis time of 5 h. The temperature × hydrolysis time interaction (AC) was non-significant (p > 0.05), indicating that the two variables exert independent main effects rather than acting synergistically. The model followed an additive response topology; whereby peak antioxidant activity is predicted when temperature and hydrolysis time are set concurrently at their individually optimal values. These findings demonstrate that enzyme concentration, temperature, and hydrolysis time independently govern the antioxidant activity of papain-hydrolysed tilapia protein, providing a basis for optimising hydrolysis conditions to maximise bioactive peptide yield.},
keywords = {Tilapia protein, physicochemical parameters, antioxidant},
month = {September},
}