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Response of OPV Corn (Zea mays) to Antitranspirants (Chitosan) Under Drought Stress
Subject area: Agriculture and Veterinary Sciences · Area of research: Agriculture
DOI: 10.64388/IREV10I4-1723642
Abstract
Chitosan is gaining recognition in crop science as a natural antitranspirant that improves plant water-use efficiency and enhances resilience under drought stress. This study investigated the effects of chitosan derived from golden apple snail shells on the growth, physiological response, and yield performance of glutinous open-pollinated variety (OPV) corn (Zea mays) under drought conditions. Conducted from February 23 to April 29, 2025, in Macatoc, Victoria, Oriental Mindoro, the experiment followed a 3×4 factorial design using a Randomized Complete Block Design, with Factor A being water stress intervals (regular, 15 days, and 25 days) and Factor B being chitosan levels (0, 50, 100, and 150 ml/L). Results showed that the first factor (water stress interval) had a significant effect (p<0.05) on key growth traits such as plant height, number of leaves, leaf area, and fresh and dry biomass, with regular watering (A1) producing superior values compared to 15-day (A2) and 25-day (A3) intervals. However, yield components like corn ear diameter, length, and weight showed comparable results between A1 and A2, suggesting moderate water stress did not drastically affect reproductive traits. The second factor (chitosan level) significantly influenced all parameters, with 100 ml/L (B3) producing the best performance in plant height, leaf area, number of leaves, biomass, corn ear traits, Relative Water Content (RWC), and Excised Leaf Water Retention (ELWR). The 150 ml/L treatment (B4) also improved traits but with diminishing gains or slight physiological suppression. The interaction between water stress intervals and chitosan levels was significant for plant height, number of leaves, leaf area, and RWC, especially at 6 and 8 weeks after planting. Notably, the combination of 100 ml/L chitosan and 15-day water stress interval (A2B3) showed performance comparable to the regularly watered treatment, indicating chitosan’s mitigating role under moderate drought. These findings support the application of chitosan at optimal concentrations as an eco-friendly, biodegradable strategy to enhance maize performance under water-limited conditions, reinforcing its value in climate-smart agriculture.
Keywords
Excised Leaf Water Retention, Growth performance, OPV Corn, Relative water content, Yield parameters
References
[1] Abdel-Aziz, S. M., et al. (2006). The effect of chitosan on growth and stress tolerance in plants under drought conditions. Arab Journal of Biotechnology, 9(2), 311- 322.
[2] Ali, M. H., El-Sayed, H. M., & El-Nagar, G. R. (2017). Effect of irrigation intervals and foliar application of micronutrients on maize growth and productivity. Journal of Plant Production, 8(9), 875- 881.
[3] Almeida, L. G., Magalhes, P. C., & Karam, D. (2018). Chitosan application in the induction of water deficit tolerance in maize plants.
[4] Anjum, S. A., et. Al.,(2011). Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research, 6(9), 2026-2032.
[5] Blum, A. (2011a). Drought resistance is it really a complex trait. Functional Plant Biology, 38(10), 753 - 757.
[6] Boonlertnirun, S., Suvannasara, R., & Promsomboon, P. (2011). Application of chitosan for reducing chemical fertilizer uses in waxy corn growing. Thai Journal of Agricultural Science, 44(5), 22-28.
[7] Cerpacio, R. V. (2004). Maize in the Philippines: Production Systems Constraints, and research priorities.
[8] Chaves, M. M., Maroco, J. P., & Pereira, J. S. (2003). Understanding plant responses to drought-from genes to the whole plant. Functional Plant Biology, 30(3), 239-264.
[9] DA PhilRice- Philippine Rice Research Institute. (2019). Golden apple snail: A major pest in Philippine rice fields. https://www.philrice.gov.ph.
[10] El Hadrami, A., Adam, L. R., El Hadrami, I., & Daayf, F. (2010). Chitosan in plant protection. Marine Drugs, 8(4), 968-987.
[11] El-Tanahy, A. M., Fawzy, Z. F., & Abd El-Rhman, I. E. (2012). Response of tomato plants to chitosan and amino acids foliar application under different levels of nitrogen fertilizer. Journal of Applied Sciences Research, 8(2), 1131-1137.
[12] Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., & Basra, S. M. A. (2009). Plant drought stress: Effects, mechanisms and management. Agronomy for Sustainable Development, 29, 185-212. https://doi.org/10.1051/agro:2008021.
[13] Farouk, S., & Amany, A. A. (2012). Improving growth and yield of cowpea by foliar application of chitosan under water stress. Egyptian Journal of Biology, 14, 14-26.
[14] Farouk, S., Mosa, A. A., & Taha, A. A. (2011). Protective effect of humic acid and chitosan on radish (Raphanus sativus L.) plants subjected to cadmium stress. Journal of Stress Physiology & Biochemistry, 7(3), 121-136.
[15] Flexas, J., & Medrano, H. (2002). Drought-inhibition of photosynthesis in C3 plants: stomatal and non-stomatal limitations revisited. Annals of Botany, 89(2), 183-189.
[16] Gopal, J., & Muthu, M. (2019). Sustainable ecofriendly phytoextract mediated one pot green recovery of chitosan. Scientific Reports.
[17] Gornik, K. et.al., (2008b). The effect of chitosan on rooting of grapevine cuttings and on subsequent plant growth under drought and temperature stress. Journal of Fruit and Ornamental Plant Research, 16(3), 333-343.
[18] Guan, Y.et.al., (2009). Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Seed Science and Technology, 37(2), 501-510.
[19] Hadwiger, L. A. (2013). Multiple effects of chitosan on plant systems: solid science or hype. Plant Science, 208, 42-49.
[20] Hassnain, A. B. et.al., (2019). Efficacy of chitosan on performance of tomato (Lycopersicon esculentum L.) plant under water stress condition. Pakistan Journal of Agriculture Research.
[21] Hidangmayum, A.et.al., (2019). Application of chitosan on seed and plant growth attributes in maize (Zea mays L.). International Journal of Current Microbiology and Applied Sciences, 8(1), 1027-1036. https://doi.org/10.20546/ijcmas.2019.801.112.
[22] Hsiao, T. C., Acevedo, E., Fereres, E., & Henderson, D. W. (2000). Stress metabolism: Water stress, growth, and osmotic adjustment. In D. W. Rains, R. C. Valentine, A. Hollaender, & A. San Pietro (Eds.), Genetic engineering of osmoregulation: Impact on plant productivity for food, chemicals, and energy (pp. 281-305).
[23] Iriti, M., & Faoro, F. (2009). Chitosan as a MAMP, searching for a PRR. Plant Signaling & Behavior, 4(1), 66-68. https://doi.org/10.4161/psb.4.1.7404
[24] Islam, M. S., Rahman, M. M., & Hasan, M. M. (2017). Properties and agricultural applications of chitosan derived from crustacean shells. Journal of Natural Polymers, 8(4), 233-245.
[25] Kang, S.et.al., (2011). Effects of limited irrigation on yield and water use efficiency of maize in the Loess Plateau of China. Agricultural Water Management, 78(3), 241-252. https://doi.org/10.1016/S0378-3774(05)00064-6.
[26] Katiyar, A., Singh, R., & Sharma, P. (2015). Role of chitosan as a biostimulant for improving drought tolerance in plants. Plant Physiology and Biochemistry, 95, 34-42.
[27] Khan, W., Prithiviraj, B., & Smith, D. L. (2002). Chitosan and chitinolytic compounds: Plant growth promoters and elicitors of defense response. Canadian Journal of Plant Science, 82(3), 381-389. https://doi.org/10.4141/P01-038.
[28] Khan, W., Prithiviraj, B., & Smith, D. L. (2002). Effect of foliar application of chitin and chitosan on plant growth promotion and yield of soybean. Journal of Plant Nutrition, 25(1), 91-101.
[29] Khan, W., Prithiviraj, B., & Smith, D. L. (2002). Effect of foliar application of chitin and chitosan oligosaccharides on photosynthesis of maize and soybean. Photosynthetica, 40, 621-624.
[30] Khan, M. N., Mobin, M., Abbas, Z., & Alamri, S. A. (2020). Role of chitosan and its derivatives in abiotic stress tolerance in plants: Recent developments. International Journal of Biological Macromolecules, 164, 2088-2100. https://doi.org/10.1016/j.ijbiomac.2020.07.160
[31] Khan, M. N.et.al.,(2020). Chitosan as a biostimulant in horticulture. Scientia Horticulturae, 274, 109506. https://doi.org/10.1016/j.scienta.2020.109506
[32] Khan, W. M., Ali, S., Rizwan, M., Noureen, S.,(2020). Chitosan enhances drought tolerance in maize by improving physiological and biochemical attributes. Environmental Science and Pollution Research, 27(12), 13461-13471.
[33] Khatun, A., Kabir, M. H., & Islam, M. R. (2016). Response of maize to water stress at different growth stages. Progressive Agriculture, 27(3), 274-281. https://doi.org/10.3329/pa.v27i3.32182.
[34] Kimi M., Hamdi M.H., (2023). Direct extraction of chitosan from snail shells by natural deep eutectic solvent. Universiti Malaysia Sarawak. Current Chemistry Letters 12 (2023) 275-280.
[35] Kocieka, J., Liberacki, D., & Strozecki, M. (2023). The role of antitranspirants in mitigating drought stress in plants of the grass family (Poaceae). A review. Sustainability. https://doi.org/10.3390/su15129165.
[36] Kumar, M. N. V. R., Muzzarelli, R. A. A., Muzzarelli, C.,(2009). Chitosan chemistry and pharmaceutical perspectives. Chemical Reviews, 104(12), 6017- 6084.
[37] Kumaraswamy, R. V.et.al.,(2020). Engineered chitosan-based nanomaterials: Mechanisms and applications in plants. International Journal of Biological Macromolecules, 165, 2952-2966.
[38] Kumaraswamy, R. V. (2014). Foliar application of chitosan improves growth and yield attributes in rice. In Application of Chitosan in Rice Production.
[39] Larasati, D. F., Priyanto, A. D., & Widodo, S. (2023). Characterization of chitosan from golden apple snail shells and its potential agricultural applications. Journal of Biopolymer Research, 16(1), 55-64.
[40] Li, Y., Xu, Y., & Chen, Y. (2020). Effects of drought regimes on growth and physiological traits of a typical shrub species in subtropical China. Global Ecology and Conservation.
[41] Lopes, M. S., Reynolds, M. P., Jalal-Kamali, M. R., Moussa, M., Feltaous, Y., (2011). The role of drought tolerance in wheat improvement. Crop and Pasture Science, 62(4), 303-311.
[42] Mondal, M. M. A.,(2012). Effect of foliar application of chitosan on growth and yield in mungbean. International Journal of Agriculture and Biology, 14(6), 1101-1105.
[43] Mondal, M. M. A.,(2013). Foliar application of chitosan alters the growth and yield of maize (Zea mays L.). International Journal of Agronomy and Plant Production, 4(11), 2555-2560.
[44] Mondal, M. M. A., & Putch, A. B. (2013). Foliar application of chitosan improves growth and yield in maize. Journal of Food, Agriculture & Environment, 11(2), 520-523.
[45] Nanyang Technological University, Singapore. (2020). Microbial extraction of chitin from seafood waste using sugars derived from fruit waste-stream. Retrieved from http://www.ntu.edu.sg.
[46] Oosterhuis, D. M., Loka, D. A., & Raper, T. B. (2007). Physiological response of maize to water deficit stress. Proceedings of the World Cotton Research Conference, 5, 30-35.
[47] Pongprayoon, W., Siringam, T., Panya, A., & Roytrakul, S. (2020). Application of chitosan in plant defense responses to biotic and abiotic stress. https://doi.org/10.14416/j.asep.2020.12.07.
[48] Riaz, A., Nawaz, H., & Cheema, M. A. (2013). Effect of water stress on growth and yield components of maize varieties. Journal of Animal and Plant Sciences, 23(5), 1450-1455.
[49] Rouphael, Y., & Colla, G. (2020). Biostimulants in agriculture. Frontiers in Plant Science, 11, 40. https://doi.org/10.3389/fpls.2020.00040.
[50] Sánchez-Rodríguez, E., Rubio-Wilhelmi, M. M., Cervilla, L. M., Blasco, B., Rios, J. J., Rosales, M. A.,& Romero, L. (2010). Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants. Plant Science, 178(1), 30-40.
[51] Sathiyabama, M., & Manikandan, A. (2018). Chitosan–silver nanoparticle composite enhances resistance to drought stress in maize plants. Biocatalysis and Agricultural Biotechnology, 15, 77-83.
[52] Scheffé, H. (1953). A method for judging all contrasts in the analysis of variance. Biometrika, 40(1-2), 87-104.
[53] Sharif, R.et.al., (2018). The multifunctional role of chitosan in horticultural crops; a review. Saudi Journal of Biological Sciences, 25(4), 709-720.
[54] Tardieu, F. (2012). Any trait or trait-related allele can confer drought tolerance: just design the right drought scenario. Journal of Experimental Botany, 63(1), 25-31.
[55] Wang, B., Liu, C., & Zhang, D. (2019). Effects of maize organ-specific drought stress response on yields from transcriptome analysis. BMC Plant Biology.
[56] Zayed, M.et.al., (2017). Effect of chitosan and potassium silicate on drought tolerance of maize plants. Zagazig Journal of Agricultural Research, 44(4), 1407-1419.
[57] Zeng, D., & Luo, X. (2012). Physiological effects of chitosan as a biostimulant on plants. International Journal of Agriculture and Biology, 14(3), 533-538.
[58] Zhang, Y.et.al.,(2018). Effects of water deficit on stem mechanical strength and its relationship with lignin accumulation in maize. Scientific Reports.
[59] Zhou, S.et.al.,(2018). How should we model plant responses to drought? An analysis of stomatal and non-stomatal limitations.
[60] Zlatev, Z. S., & Lidon, F. C. (2012). An overview on drought induced changes in plant growth, water relations and photosynthesis. Emirates Journal of Food and Agriculture, 24(1), 57-72.
How to cite this paper
@article{1723642,
author = {Sharlene D. Andaya},
title = {Response of OPV Corn (Zea mays) to Antitranspirants (Chitosan) Under Drought Stress},
journal = {Iconic Research And Engineering Journals},
year = {2026},
volume = {10},
number = {4},
pages = {603-627},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1723642.pdf},
abstract = {Chitosan is gaining recognition in crop science as a natural antitranspirant that improves plant water-use efficiency and enhances resilience under drought stress. This study investigated the effects of chitosan derived from golden apple snail shells on the growth, physiological response, and yield performance of glutinous open-pollinated variety (OPV) corn (Zea mays) under drought conditions. Conducted from February 23 to April 29, 2025, in Macatoc, Victoria, Oriental Mindoro, the experiment followed a 3×4 factorial design using a Randomized Complete Block Design, with Factor A being water stress intervals (regular, 15 days, and 25 days) and Factor B being chitosan levels (0, 50, 100, and 150 ml/L). Results showed that the first factor (water stress interval) had a significant effect (p<0.05) on key growth traits such as plant height, number of leaves, leaf area, and fresh and dry biomass, with regular watering (A1) producing superior values compared to 15-day (A2) and 25-day (A3) intervals. However, yield components like corn ear diameter, length, and weight showed comparable results between A1 and A2, suggesting moderate water stress did not drastically affect reproductive traits. The second factor (chitosan level) significantly influenced all parameters, with 100 ml/L (B3) producing the best performance in plant height, leaf area, number of leaves, biomass, corn ear traits, Relative Water Content (RWC), and Excised Leaf Water Retention (ELWR). The 150 ml/L treatment (B4) also improved traits but with diminishing gains or slight physiological suppression. The interaction between water stress intervals and chitosan levels was significant for plant height, number of leaves, leaf area, and RWC, especially at 6 and 8 weeks after planting. Notably, the combination of 100 ml/L chitosan and 15-day water stress interval (A2B3) showed performance comparable to the regularly watered treatment, indicating chitosan’s mitigating role under moderate drought. These findings support the application of chitosan at optimal concentrations as an eco-friendly, biodegradable strategy to enhance maize performance under water-limited conditions, reinforcing its value in climate-smart agriculture.},
keywords = {Excised Leaf Water Retention, Growth performance, OPV Corn, Relative water content, Yield parameters},
month = {October},
doi = {https://doi.org/10.64388/IREV10I4-1723642}
}