Formulation and Evaluation of a Herbal Antioxidant Face Cream Containing Aloe vera Gel, Green Tea and Pomegranate Peel Extracts for Skin Care

References

[1] Fisher GJ, Kang S, Varani J, Bata-Csorgo Z, Wan Y, Datta S, et al. Mechanisms of photoaging and chronological skin aging. Arch Dermatol. 2002;138(11):1462–70.

[2] Rittié L, Fisher GJ. Natural and sun-induced aging of human skin. Cold Spring Harb Perspect Med. 2015;5(1):a015370.

[3] Krutmann J, Bouloc A, Sore G, Bernard BA, Passeron T. The skin aging exposome. J Dermatol Sci. 2017;85(3):152–61.

[4] Rinnerthaler M, Bischof J, Streubel MK, Trost A, Richter K. Oxidative stress in aging human skin. Biomolecules. 2015;5(2):545–89.

[5] Poljšak B, Dahmane R. Free radicals and extrinsic skin aging. Dermatol Res Pract. 2012;2012:135206.

[6] Pinnell SR. Cutaneous photodamage, oxidative stress, and topical antioxidant protection. J Am Acad Dermatol. 2003;48(1):1–19.

[7] Masaki H. Role of antioxidants in the skin: anti-aging effects. J Dermatol Sci. 2010;58(2):85–90.

[8] Aburjai T, Natsheh FM. Plants used in cosmetics. Phytother Res. 2003;17(9):987–1000.

[9] Ribeiro AS, Estanqueiro M, Oliveira MB, Sousa Lobo JM. Main benefits and applicability of plant extracts in skin care products. Cosmetics. 2015;2(2):48–65.

[10] Zillich OV, Schweiggert-Weisz U, Eisner P, Kerscher M. Polyphenols as active ingredients for cosmetic products. Int J Cosmet Sci. 2015;37(5):455–64.

[11] Mukherjee PK, Maity N, Nema NK, Sarkar BK. Bioactive compounds from natural resources against skin aging. Phytomedicine. 2011;19(1):64–73.

[12] Jadoon S, Karim S, Bin Asad MHH, Akram MR, Khan AK, Malik A, et al. Anti-aging potential of phytoextract loaded-pharmaceutical creams for human skin cell longevity. Oxid Med Cell Longev. 2015;2015:709628.

[13] Reynolds T, Dweck AC. Aloe vera leaf gel: a review update. J Ethnopharmacol. 1999;68(1–3):3–37.

[14] Hamman JH. Composition and applications of Aloe vera leaf gel. Molecules. 2008;13(8):1599–616.

[15] Surjushe A, Vasani R, Saple DG. Aloe vera: a short review. Indian J Dermatol. 2008;53(4):163–6.

[16] Dal'Belo SE, Gaspar LR, Maia Campos PMBG. Moisturizing effect of cosmetic formulations containing Aloe vera extract in different concentrations assessed by skin bioengineering techniques. Skin Res Technol. 2006;12(4):241–6.

[17] Hsu S. Green tea and the skin. J Am Acad Dermatol. 2005;52(6):1049–59.

[18] Katiyar SK, Elmets CA. Green tea polyphenolic antioxidants and skin photoprotection (review). Int J Oncol. 2001;18(6):1307–13.

[19] Elmets CA, Singh D, Tubesing K, Matsui M, Katiyar S, Mukhtar H. Cutaneous photoprotection from ultraviolet injury by green tea polyphenols. J Am Acad Dermatol. 2001;44(3):425–32.

[20] Nichols JA, Katiyar SK. Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Arch Dermatol Res. 2010;302(2):71–83.

[21] Oyetakin White P, Tribout H, Baron E. Protective mechanisms of green tea polyphenols in skin. Oxid Med Cell Longev. 2012;2012:560682.

[22] Chiu AE, Chan JL, Kern DG, Kohler S, Rehmus WE, Kimball AB. Double-blinded, placebo-controlled trial of green tea extracts in the clinical and histologic appearance of photoaging skin. Dermatol Surg. 2005;31(7 Pt 2):855–60.

[23] Heinrich U, Moore CE, De Spirt S, Tronnier H, Stahl W. Green tea polyphenols provide photoprotection, increase microcirculation, and modulate skin properties of women. J Nutr. 2011;141(6):1202–8.

[24] Li Y, Guo C, Yang J, Wei J, Xu J, Cheng S. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem. 2006;96(2):254–60.

[25] Negi PS, Jayaprakasha GK, Jena BS. Antioxidant and antimutagenic activities of pomegranate peel extracts. Food Chem. 2003;80(3):393–7.

[26] Akhtar S, Ismail T, Fraternale D, Sestili P. Pomegranate peel and peel extracts: chemistry and food features. Food Chem. 2015;174:417–25.

[27] Fischer UA, Carle R, Kammerer DR. Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD-ESI/MSn. Food Chem. 2011;127(2):807–21.

[28] Aslam MN, Lansky EP, Varani J. Pomegranate as a cosmeceutical source: pomegranate fractions promote proliferation and procollagen synthesis and inhibit matrix metalloproteinase-1 production in human skin cells. J Ethnopharmacol. 2006;103(3):311–8.

[29] Afaq F, Malik A, Syed DN, Maes D, Matsui MS, Mukhtar H. Pomegranate fruit extract modulates UV-b-mediated phosphorylation of mitogen-activated protein kinases and activation of nuclear factor kappa b in normal human epidermal keratinocytes. Photochem Photobiol. 2005;81(1):38–45.

[30] Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, et al. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem. 2005;16(6):360–7.

[31] World Health Organization. Quality control methods for medicinal plant materials. Geneva: World Health Organization; 1998.

[32] Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. 3rd ed. London: Chapman and Hall; 1998.

[33] Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am J Enol Vitic. 1965;16(3):144–58.

[34] Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10(3):178–82.

[35] Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT – Food Sci Technol. 1995;28(1):25–30.

[36] Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol. 2004;26(2):211–9.

[37] Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 2005;53(10):4290–302.

[38] Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal. 2016;6(2):71–9.

[39] Draize JH, Woodard G, Calvery HO. Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. J Pharmacol Exp Ther. 1944;82(3):377–90.

[40] International Conference on Harmonisation. ICH harmonised tripartite guideline Q1A(R2): stability testing of new drug substances and products. Geneva: ICH; 2003.

[41] Zhu QY, Zhang A, Tsang D, Huang Y, Chen ZY. Stability of green tea catechins. J Agric Food Chem. 1997;45(12):4624–8.

[42] Kaur IP, Kapila M, Agrawal R. Role of novel delivery systems in developing topical antioxidants as therapeutics to combat photoageing. Ageing Res Rev. 2007;6(4):271–88.

[43] Al-Zoreky NS. Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. Int J Food Microbiol. 2009;134(3):244–8.

@article{1723898,
      author = {Shweta Kushwaha, Pradeep Kumar},
      title = {Formulation and Evaluation of a Herbal Antioxidant Face Cream Containing Aloe vera Gel, Green Tea and Pomegranate Peel Extracts for Skin Care},
      journal = {Iconic Research And Engineering Journals},
      year = {2026},
      volume = {10},
      number = {4},
      pages = {1491-1505},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1723898.pdf},
      abstract = {Background: Oxidative stress drives both intrinsic and photo-induced skin ageing, and polyphenol-rich botanicals are widely used in topical products to counter it.
Objective: To formulate and evaluate an oil-in-water (O/W) herbal face cream combining Aloe barbadensis gel, Camellia sinensis (green tea) leaf extract and Punica granatum (pomegranate) peel extract.
Methods: Green tea (Soxhlet, 95% ethanol) and pomegranate peel (maceration, 70% ethanol) extracts and clarified aloe gel were characterised by phytochemical screening and by total phenolic, flavonoid and tannin assays. Five creams (F1–F5) with increasing extract loads were prepared by fusion–emulsification and assessed for organoleptic and physicochemical properties, DPPH radical scavenging (10–160 µg/mL; ascorbic acid as reference), antimicrobial activity (agar well diffusion), Draize patch-test safety, centrifugation and freeze–thaw behaviour, and 90-day stability under ICH Q1A(R2) conditions.
Results: Pomegranate peel extract had the highest phenolic (342.60 ± 7.48 mg GAE/g) and tannin content, and green tea extract the highest flavonoid content. Antioxidant potency rose with extract load (IC50 74.33 µg/mL for F1 to 32.29 µg/mL for F5; ascorbic acid 29.13 µg/mL), whereas viscosity fell and the highest-load batch (F5) showed grittiness and slight creaming. Batch F4 (10% aloe gel, 5% green tea extract, 2.5% pomegranate peel extract) gave the best composite score: pH 5.78 ± 0.04, viscosity 18,900 ± 410 cP, spreadability 9.38 ± 0.26 g·cm/s, IC50 35.42 µg/mL, a primary irritation index of 0.033, and retention of 92.4% of its antioxidant activity after 90 days at 40 °C/75% RH. Its IC50 was about 1.8-fold lower than that of a marketed herbal cream.
Conclusions: The three-extract cream F4 is a stable, well-tolerated and antioxidant-active cosmeceutical candidate; in vivo efficacy studies and marker-based standardisation are the next steps.},
      keywords = {herbal cosmeceutical; antioxidant cream; Aloe vera; green tea; pomegranate peel; DPPH; stability; skin ageing},
      month = {October},
  }