Bacteriological Analysis/Bioburden Evaluation of Bedsheets of Students in Ambrose Alli University Ekpoma

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@article{1724497,
      author = {AJAYI, Damilola Elizabeth},
      title = {Bacteriological Analysis/Bioburden Evaluation of Bedsheets of Students in Ambrose Alli University Ekpoma},
      journal = {Iconic Research And Engineering Journals},
      year = {2024},
      volume = {7},
      number = {7},
      pages = {850-874},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1724497.pdf},
      abstract = {Bedsheets may serve as reservoirs for microorganisms transferred from human skin, bodily secretions, and the surrounding environment, potentially contributing to microbial transmission and antimicrobial resistance. This study evaluated the bacteriological contamination and bioburden of bedsheets used by students of Ambrose Alli University, Ekpoma, Edo State, Nigeria, with emphasis on bacterial identification, virulence characteristics, antimicrobial susceptibility, and hygiene practices. Eighteen bedsheet samples were collected in August 2023 using sterile swab techniques, while questionnaires were administered to obtain information on respondents’ bedsheet hygiene practices. Samples were processed using conventional bacteriological procedures, including serial dilution, pour-plate enumeration, cultural and biochemical identification, molecular characterization procedures, phenotypic virulence testing, and antibiotic susceptibility testing using the Kirby–Bauer disc diffusion method. The multiple antibiotic resistance index was also evaluated. The findings revealed the presence of four bacterial species: Escherichia coli, Enterobacter cloacae, Bacillus mycoides, and Serratia marcescens. The reported mean heterotrophic bacterial counts were 8.5 and 7.25 at the anterior and posterior ends of the bedsheets, respectively. All four isolates exhibited haemolytic activity and lipase production, whereas none demonstrated DNase activity. Antimicrobial susceptibility patterns varied among the isolates, with E. cloacae showing resistance to four tested antibiotics and E. coli exhibiting variable resistance. The study also identified inconsistencies in bedsheet hygiene practices, including infrequent washing and the use of mattresses without bedsheets. These findings highlight the potential of student bedsheets to harbour viable bacteria with concerning virulence and antimicrobial resistance characteristics. The study recommends regular bedsheet laundering, improved personal and environmental hygiene, and continued microbiological surveillance to promote healthier residential environments and reduce potential microbial transmission among university students.},
      keywords = {Bedsheet contamination, bacterial bioburden, antimicrobial resistance, virulence characteristics, hygiene practices},
      month = {January},
  }