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Evaluation of Temperature Effects on Biokinetic Functional Parameters of Petroleum Hydrocarbon Degradation in Freshwater Environment
Subject area: Science,Engineering and Technology · Area of research: Environmental Engineering
Abstract
This study investigated the influence of temperature on the biokinetic functional parameters governing total petroleum hydrocarbon (TPH) degradation in freshwater environments. The research employed the Monod kinetic model to describe the relationship between substrate concentration and degradation rate, while Lineweaver–Burk analysis was used to evaluate the maximum specific degradation rate and substrate affinity-related kinetic constant. Temperature-dependent kinetic behaviour was examined at 15, 30, 45, 60 and 75 °C. The reciprocal relationship between degradation velocity and substrate concentration was evaluated using linear regression equations obtained from the corresponding Lineweaver–Burk plots. The estimated maximum velocity parameters (Vmax) varied considerably with temperature, with reported values of 285.71, 333.33, 1666.67, 500, 500, 50,000 and 166.67 (ppm/hr)⁻¹ at 15, 30, 45, 60 and 75 °C, respectively. The corresponding reported kinetic constants also exhibited substantial variations, indicating a temperature-dependent response of the degradation system. In addition, modified sedimentation relationships based on Stokes' and Newton's laws were formulated to incorporate oil-particle characteristics into the analysis of hydrocarbon behaviour in water. The findings demonstrate that temperature significantly influences the estimated biokinetic parameters and the mathematical description of TPH degradation in freshwater. The study provides a modelling framework for understanding hydrocarbon degradation behaviour and supporting the engineering assessment of petroleum-contaminated aquatic environments. However, further experimental validation is necessary to establish the biological significance of the unusually high-temperature kinetic estimates and verify the consistency of the reported parameters.
Keywords
biokinetics, freshwater environment, temperature effect, total petroleum hydrocarbons, Monod equation, Lineweaver–Burk plot, sedimentation, petroleum pollution
References
[1] Atlas RM. Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol Rev. 1981;45(1):180–209.
[2] Lineweaver H, Burk D. The determination of enzyme dissociation constants. J Am Chem Soc. 1934;56(3):658–666.
[3] Monod J. The growth of bacterial cultures. Annu Rev Microbiol. 1949;3:371–394.
[4] White FM. Fluid mechanics. 8th ed. New York: McGraw-Hill Education; 2016.
[5] Ozioko FC, Ibezim NE, Okirie FU. Comparative evaluation of adsorption and biodegradation mechanisms in PMS-contaminated water using clay and banana-based adsorbent. Int J Pharmacogn Investig Technol. 2026;2(1):50–57.
[6] Kingdom UM, Nkwachi UP, Chinyeze WB. Kinetic and isotherm evaluation of oil-polluted water treatment using low-cost adsorbent materials: implications for Niger Delta environmental remediation. Journal of Environmental Engineering and Studies. 2026;11(2):1–9.
[7] Chidiebere OF, Kingdom UM, Orluma CG. Comparative evaluation of physicochemical parameter dynamics during crude oil degradation in freshwater and saltwater media. Journal of Water Resources and Pollution Studies. 2026;11(1):25–33.
[8] Okechukwu C, Okirie FU, Kingdom UM. Comparison of crude oil degradation in freshwater and saltwater environments using batch reactors. International Journal of Hydraulics and Wastewater Treatment Technologies. 2026;2(1):1–11.
[9] Yadav RC, Srivastava VC. Physicochemical properties of the water of river Ganga at Ghazipur. Indian Journal of Scientific Research. 2011;2:41–44.
[10] Ukpaka CP, Douglas IE. Model development for pH, salinity and conductivity monitoring and predicting the diffusion concentration in stagnant water. Chemical Science Journal. 2016;7(1):1–5.
How to cite this paper
@article{1724927,
author = {Kingdom Matthew Umah, Wali Obinuchi, Patience Ikenyiri},
title = {Evaluation of Temperature Effects on Biokinetic Functional Parameters of Petroleum Hydrocarbon Degradation in Freshwater Environment},
journal = {Iconic Research And Engineering Journals},
year = {2026},
volume = {10},
number = {5},
pages = {1842-1852},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1724927.pdf},
abstract = {This study investigated the influence of temperature on the biokinetic functional parameters governing total petroleum hydrocarbon (TPH) degradation in freshwater environments. The research employed the Monod kinetic model to describe the relationship between substrate concentration and degradation rate, while Lineweaver–Burk analysis was used to evaluate the maximum specific degradation rate and substrate affinity-related kinetic constant. Temperature-dependent kinetic behaviour was examined at 15, 30, 45, 60 and 75 °C. The reciprocal relationship between degradation velocity and substrate concentration was evaluated using linear regression equations obtained from the corresponding Lineweaver–Burk plots. The estimated maximum velocity parameters (Vmax) varied considerably with temperature, with reported values of 285.71, 333.33, 1666.67, 500, 500, 50,000 and 166.67 (ppm/hr)⁻¹ at 15, 30, 45, 60 and 75 °C, respectively. The corresponding reported kinetic constants also exhibited substantial variations, indicating a temperature-dependent response of the degradation system. In addition, modified sedimentation relationships based on Stokes' and Newton's laws were formulated to incorporate oil-particle characteristics into the analysis of hydrocarbon behaviour in water. The findings demonstrate that temperature significantly influences the estimated biokinetic parameters and the mathematical description of TPH degradation in freshwater. The study provides a modelling framework for understanding hydrocarbon degradation behaviour and supporting the engineering assessment of petroleum-contaminated aquatic environments. However, further experimental validation is necessary to establish the biological significance of the unusually high-temperature kinetic estimates and verify the consistency of the reported parameters.},
keywords = {biokinetics, freshwater environment, temperature effect, total petroleum hydrocarbons, Monod equation, Lineweaver–Burk plot, sedimentation, petroleum pollution},
month = {November},
}