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Histologic Evalution of Adult Wistar Rats Kidney Exposed to Generator Fumes, Petrol, Herbicides, Electrocution and Starvation for Infarction
Subject area: Biological & Medical Sciences · Area of research: Histology
Abstract
Introduction: The increasing human population has led to the use of agrochemicals for weeding which improve food production, likewise the use of fuel and other amenity which has exposure human to various stressors is on the increase. Aim: This study histologically assessed the effect of these various stressors such as generator fumes, petrol, paraquat herbicides, electrocution and starvation in Adult Wistar rats’ kidneys. Methodology: It was centered on 42 rats, randomly divided into six groups (1-6): control (normal room air), exposed to generator fumes, paraquat herbicides, electrocution, starvation and petrol respectively. The weight of each rat was recorded and analysed using one-way analysis of variance (ANOVA) for significant difference between each stressor group against control. At interval, the rats were sacrificed, kidneys were harvested, tissue processed, photomicrographs were reported. Result: Findings demonstrate significant weight loss in starvation, and no much changes among other groups except the petrol-exposed groups. The histologic architecture of the rat's kidney placed on starvation showed acute renal injury, while kidney of generator fumes had peritubular spaces and a darker glomerulus when stained with Masson trichrome probably due to carbon monoxide. An irregular vascular wall pointing to an acute kidney and glomerular injury showing signs of infarction when observed in the electrocution group. Conclusion: There was no significant weight loss in rats exposed to petrol, paraquat, generator fumes, and electrocution, but starvation led to a marked reduction in body weight and death. Histological assessment showed signs of acute kidney injury such as tubular damage, dilated capillaries, and glomerular changes especially in the generator fume, electrocution, and starvation groups. In contrast, the kidneys of rats in the petrol, paraquat, and control groups appeared normal. Both Hematoxylin and Eosin and Masson’s Trichrome stains showed similar results, with no signs of fibrosis, supporting the accuracy and consistency of the observed changes.
Keywords
Stressors, kidney, histology, starvation, electrocution
References
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How to cite this paper
@article{1725056,
author = {Ehebha E. Santos, Ajemre Larry, Egbowawa U. Pearl, Ahama E. Efe, Omashim Kaine},
title = {Histologic Evalution of Adult Wistar Rats Kidney Exposed to Generator Fumes, Petrol, Herbicides, Electrocution and Starvation for Infarction},
journal = {Iconic Research And Engineering Journals},
year = {2026},
volume = {10},
number = {5},
pages = {2615-2626},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1725056.pdf},
abstract = {Introduction: The increasing human population has led to the use of agrochemicals for weeding which improve food production, likewise the use of fuel and other amenity which has exposure human to various stressors is on the increase. Aim: This study histologically assessed the effect of these various stressors such as generator fumes, petrol, paraquat herbicides, electrocution and starvation in Adult Wistar rats’ kidneys. Methodology: It was centered on 42 rats, randomly divided into six groups (1-6): control (normal room air), exposed to generator fumes, paraquat herbicides, electrocution, starvation and petrol respectively. The weight of each rat was recorded and analysed using one-way analysis of variance (ANOVA) for significant difference between each stressor group against control. At interval, the rats were sacrificed, kidneys were harvested, tissue processed, photomicrographs were reported. Result: Findings demonstrate significant weight loss in starvation, and no much changes among other groups except the petrol-exposed groups. The histologic architecture of the rat's kidney placed on starvation showed acute renal injury, while kidney of generator fumes had peritubular spaces and a darker glomerulus when stained with Masson trichrome probably due to carbon monoxide. An irregular vascular wall pointing to an acute kidney and glomerular injury showing signs of infarction when observed in the electrocution group. Conclusion: There was no significant weight loss in rats exposed to petrol, paraquat, generator fumes, and electrocution, but starvation led to a marked reduction in body weight and death. Histological assessment showed signs of acute kidney injury such as tubular damage, dilated capillaries, and glomerular changes especially in the generator fume, electrocution, and starvation groups. In contrast, the kidneys of rats in the petrol, paraquat, and control groups appeared normal. Both Hematoxylin and Eosin and Masson’s Trichrome stains showed similar results, with no signs of fibrosis, supporting the accuracy and consistency of the observed changes.},
keywords = {Stressors, kidney, histology, starvation, electrocution},
month = {November},
}