International Peer-Reviewed JournalOpen AccessISSN 2456-8880
irejournals@gmail.com+91-7433024337

Home / Current Issue / Paper 1705065

1705065 Vol 7 · Issue 3 Download Paper

Incidence and Antibiotic Resistance Pattern of Klebsiella Species Isolated from Poultry Feaces in Owo Metropolis

Adeluwoye-Ajayi, Olayemi Amos Bello, Adedoyin Ayowole

Subject area: Science,Engineering and Technology  ·  Area of research: Antibiotics Resistance

Abstract

The wrong and excessive use of antibiotics in the poultry industry poses a threat to public health due to the release of antibiotic-resistant bacteria into the environment and its impact on consumers of poultry products. This research aimed to investigate the occurrence and patterns of antibiotic resistance found in Klebsiella species isolated from poultry faeces in Owo metropolis. Fecal samples were collected from healthy broilers in three privately-owned poultry farms in Owo, using sterile universal bottles and spatulas. Klebsiella species were isolated using MacConkey Agar, and their characteristics on Nutrient Agar were also observed. The isolated Klebsiella species exhibited different characteristics on different media types. All samples from the three farms contained Klebsiella species that were resistant to AUG and CAZ were susceptible to GEN, IMP, AZT, CIP, and NIT. A MARI score of 0.29 was obtained which indicates the presence of multidrug-resistant Klebsiella species in the bird excrement from poultry farms in Owo. Consequently, poultry breeders in Owo should exercise caution when employing antibiotics.

References

[1] Abbot, S. L. (2007). Klebsiella, Enterobacter, Citrobacter, Serratia, Plesiomonas, and others Enterobacteriacee. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry and M. A. Pfaller (Eds.), Manual of Clinical Microbiology (9th edition., page, 698-711). Washinton, USA: American Society of Microbiology Press.

[2] Adelowo, O. O., Fagade, O. E. and Agerso, Y. (2014). Antibiotic resistance and resistance genes in Escherichia coli from poultry farms, southwest Nigeria. Journal of Infection in Developing Countries, 8, 1103-1112.

[3] Agalu, A., Denboba, A. A. and Mekonnen, A. (2014). “Prevalence and antibiotic resistance pattern of urinary tract bacterial infections in Dessie area, North-East Ethiopia,” BMC ResearchNotes, 19, 687.

[4] Agyare, C., Boamah, V. E., Zumbi, C. N., & Osei, F. B. (2018). Antibiotic use in poultry production and its effects on bacterial resistance. Antimicrobial resistance-A global threat, 1-20.

[5] Akbar, A. and Anal, K. A. (2013). Prevalence and antibiogramstudy of Salmonella and Staphylococcus aureusin poultry meat. Asian Pacific Journal of Tropical Biomedicine, 3 (2), 163-168.

[6] Anderson, T. C., Nguyen, T. A., Adams, J. K., Garrett, N. M., Bopp, C. A., Baker, J. B., McNeil, C., Torres, P., Ettestad, P. J. and Erdman, M. M. (2016). Multistate outbreak of human Salmonella typhimurium infections linked to live poultry from agricultural feed stores and mail-order hatcheries, United States 2013. One Health, 2, 144–149.

[7] Aniokette, U., Iroha, C., Ajah, M. and Nwakaeze, A. (2016). Occurrence of multi-drug resistant Gram-negative bacteria from poultry and poultry products sold in Abakaliki. Journal of Agricultural Science and Food Technology, 2, 119-124.

[8] Awogbemi, J., Adeyeye, M. and Akinkunmi, E. O. (2018). A survey of antimicrobial agents usage in poultry farms and antibiotic resistance in Escherichia coli and Staphylococci isolates from the poultry in Ile-Ife, Nigeria. Journal of Infectious Diseases and Epidemiology 4, 047.

[9] Blaak, H., Hamidjaja, R. A., van Hoek, A. H., de Heer, L., de Roda Husman, A. M. and Schets, F. M. (2014). Detection of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli on flies at poultry farms. Applied Environmental Microbiology, 80, 239-246.

[10] Blaak, H., van Hoek, A. H., Hamidjaja, R. A., van der Plaats, R. Q., Kerkhof-de Heer, L., de Roda Husman, A. M., & Schets, F. M. (2015). Distribution, numbers, and diversity of ESBL-producing E. coli in the poultry farm environment. PloS one, 10(8), e0135402.

[11] Boamah, V. E., Agyare, C., Odoi, H. and Dalsgaard, A. (2016). Antibiotic practices and factors influencing the use of antibiotics in selected poultry farms in Ghana. J ournal on Antimicrobial Agents, 2, 120-128.

[12] Boeckel, T. P. V., Brower, C., Gilbert, M., Grenfell, B. T., Levin, S. A., Robinsoni, T. P., Teillant, A. and Laxminarayan, R. (2015). Global trends in antimicrobial use in food animals. Agricultural Sciences 112 (18), 5649-5654.

[13] Bolan, N. S., Szogi, A. A., Chuasavathi T., Seshadri, B. V., Rothrock, M. J. and Panneerselvam, P. (2010). Uses and management of poultry litter. World Poultry Science Journal, 66, 673-698.

[14] Börjesson, S., Jernberg, C., Brolund, A., Edquist, P., Finn, M., Landén, A., ... & Englund, S. (2013). Characterization of plasmid‐mediated A mpC‐producing E. coli from S wedish broilers and association with human clinical isolates. Clinical Microbiology and Infection, 19(7), E309-E311.

[15] Chander, A. and Shrestha, C. D. (2013). “Prevalence of extended spectrum beta lactamase producing Escherichia coli and Klebsiella pneumoniae urinary isolates in a tertiary care hospital in Kathmandu, Nepal,” BMC Research Notes, 6 (1),. 487.

[16] Cheesbrough, M. (2010). Biochemical tests to identify bacteria. In: District laboratory practice in tropical countries (part 2). Cambridge University Press, Cambridge, UK. Page, 71-76.

[17] Cheng, F., Li, Z., Lan, S., Liu, W., Li, X., Zhou, Z., Song, Z., Wu, J., Zhang, M. and Shan, W. (2018). Characterization of Klebsiella pneumoniae associated with cattle infections in southwest China using multi-locus sequence typing (MLST), antibiotic resistance and virulence-associated gene profile analysis. Brazilian Journal of Microbiology, 49, 93–100.

[18] Cheng, F., Li, Z., Lan, S., Liu, W., Li, X., Zhou, Z., Song, Z., Wu, J., Zhang, M and Shan, W. (2018). Characterization of Klebsiella pneumoniae associated with cattle infections in southwest China using multi-locus sequence typing (MLST), antibiotic resistance and virulence-associated gene profile analysis. Brazilian Journal of Microbiology, 49, 93–100.

[19] Clinical and Laboratory Standard Institute (CLSI), (2020). Performance Standards for Antimicrobial Susceptibility Testing. Twenty-eighth Information Supplement CLSI document M100-S26. Wayne, P.A. 38 (3), 1-296.

[20] Davis, G. S., Waits, K., Nordstrom, L, et al. (2018). Antibiotic-resistant Escherichia coli from retail poultry meat with different antibiotic use claims. BMC Microbiology, 18, 174-177.

[21] Disinfection and Sterilization. (2005). Laboratory Biosafety Manual (3rd edition, 60-70). Geneva: WHO.

[22] Donkor, E. S., Newman, M. J. and Yeboah-Manu, D. (2012). Epidemiological aspects of nonhuman antibiotic usage and resistance: implications for the control of antibiotic resistance in Ghana. Tropical Medicine and International Health, 17, 462-468.

[23] Egea, P., López-Cerero, L., Torres, E., del Carmen Gómez-Sánchez, M., Serrano, L., Sánchez-Ortiz, M. D. N., ... & Pascual, A. (2012). Increased raw poultry meat colonization by extended spectrum beta-lactamase-producing Escherichia coli in the south of Spain. International journal of food microbiology, 159(2), 69-73.

[24] Eibach, D., Dekker, D., Boahen, K. G., Akenten, C. W., Sarpong, N., Campos, C. B., ... & May, J. (2018). Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in local and imported poultry meat in Ghana. Veterinary microbiology, 217, 7-12.7-12.

[25] Ekwealor, P. A., Ugwu, M. C., Ezeobi, I.,Amalukwe, G., Ugwu, B. C., Okezie, U., Stanley, C.,and Esimone, C. (2016). Antimicrobial evaluation of bacterial isolates from urine specimen of patients with complaints of urinary tract infections in Awka, Nigeria. International journal of Microbiology, 1-6,

[26] Emokaro, C. O., Akinrinmola, F. K. and Emokpae, O. P. (2016). Economics of backyard poultry farming in Benin City, Edo state, Nigeria. Nigeria Journal Agricultural Food Environments, 12, 50-5.

[27] Euzeby, J. P. (2000). List of bacterial names with standing in nomenclature. http://www. bacterio. cict. fr/.

[28] FAOSTAT (2018). Food and Agricultural Organization of the United Nations. Retrieved May, 19,2020 from www.fao.org/faostat/en/#data/QA.

[29] García-Vello, P., González-Zorn, B. and Saba, C. K. S. (2020). Antibiotic resistance patterns in human, animal, food and environmental isolates in Ghana: a review. Pan African MedicalJournal, 35, 37.

[30] Gebremariam, G., Legese, H., Woldu, Y., Araya, T., Hagos, K., and Gebreyesus, W. A. (2019) “Bacteriological profile, risk factors and antimicrobial susceptibility patterns of symptomatic urinary tract infection among students of Mekelle University, northern Ethiopia,” BMC Infectious Diseases, 19 (1), 950.

[31] Gregova, G., Kmetova, M., Kmet, V., Venglovsky, J. and Feher A. (2012). Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse. Annals of Agricultural and Environmental Medicine, 19, 75-77.

[32] Guetaba, M. Y. (2015). Prevalence and Antibiotic Resistance of Salmonella Sp., Shigella Sp. And Escherichia coli in Fresh Retail Chicken in the Accra Metropolis. University ofGhana;.

[33] Hedman, H. D., Vasco, K. A. and Zhang, L. (2020). A review of antimicrobial resistance in poultry farming within Low-Resource settings. Animals, 10, 1264.

[34] Jayabarath, J. (2015). Isolation and Characterization of Antibiotic Resistant Bacteria from Lutjanus campechanus. World Journal of Pharmaceutical Research, 5, 4-8.

[35] Jakobsen, L., Kurbasic, A., Skjot-Rasmussen, L, et al. (2010). Escherichia coli isolates from broiler chicken meat, broiler chickens, pork, and pigs share phylogroups and antimicrobial resistance with community-dwelling humans and patients with urinary tract infection. Foodborne Pathogenic Diseases, 7, 537-547.

[36] Janda, J. M. and Abbott, S. L. (2006). The Genera Klebsiella and Roaultella. The Enterobacteria. 2 ed. Washington, USA: American Society of Microbiology Press. 115-29.

[37] Johnson, S., Bugyei, K., Nortey, P. and Tasiame, W. (2019). Antimicrobial drug usage and poultry production: case study in Ghana. Animal Production Scence, 59 (1), 177-182.

[38] Johnson, T. J., Logue, C. M., Johnson, J. R., et al. (2012). Associations between multidrug resistance, plasmid content, and virulence potential among extraintestinal pathogenic and commensal Escherichia coli from humans and poultry. FoodbornePathogenic Disease, 9, 37-46.

[39] Klein, E. Y., Boeckel, T. P. V., Martinez, E. M., Pant, S., Gandra, S. and Levin, S. A. (2018). Global increase and geographic convergence in antibiotic consumption between 2000 and 2015. Proceedings of the National Academy of Sciences 115(15):3463-3470.

[40] Kumarasamy, K. K., Toleman, M. A., Walsh, T. R., Bagaria, J., Butt, F., Balakrishnan, R. et al. (2010). Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. The Lancet Infectious Diseases, 10 (9), 597-602.

[41] Maestre-carballa, L., Gomez, M. L., Navarro, A. A., Garcia-heredia, I., Martinez-hernandez, F., and Martinez-garcia, M. (2019). Insights into the antibiotic resistance dissemination in a wastewater effluent microbiome : bacteria, viruses and vesicles matter. Environmental Microbiology, 21(12), 4582-4596.

[42] Manyi-Loh, C., Mamphweli, S., Meyer, E. and Okoh, A. (2018). Antibiotic use in agriculture and its consequential resistance in environmental sources: potential public health implications. Molecules, 23 (4), 795.

[43] Mason, C. A. and Hamer, G. (2005). Survival and activity of Klebsiella pneumoniae at super-optimal temperatures. Bioprocess and Biosystems Engineering, 2 (3), 121-127.

[44] Mshelia, I. T., Atsanda, N. N., Bitrus, A. A., Adam, B. M., Fika, I. I. and Balami, S. B. (2016). Retrospective study of selected endemic viral diseases of poultry diagnosed in Maiduguri North- Eastern Nigeria. Journal of Animal Health Production, 4, 60-4.

[45] Muthuma, E. M., Gitau, G. K. and Aboge, G. O. (2016). Antimicrobial usage in broiler farms in, peri-urban, Nairobi, Kenya. American Journal of Research Communication, 4 (8), 14–29.

[46] Ndir, A., Diop, A., Ka, R., Faye, P. M., Dia-badiane, N. M., Ndoye, B. and Astagneau, P. (2016). Infections caused by extended-spectrum beta-lactamases producing Enterobacteriaceae: clinical and economic impact in patients hospitalized in 2 teaching hospitals in Dakar , Senegal. Antimicrobial Resistance and Infection Control, 5 (13), 1-8.

[47] Nordmann, P., Cuzon, G. and Naas, T. (2009). The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. Lancet Infectious Diseases, 9 (4), 228–236.

[48] Odoki, M., Almustapha Aliero, A., Tibyangye, J., Nyabayo Maniga, J., Wampande, E., Drago Kato, C., ... & Bazira, J. (2019). Prevalence of bacterial urinary tract infections and associated factors among patients attending hospitals in Bushenyi district, Uganda. International journal of microbiology, 2019.

[49] Omeike, S. O., Koleoso, O. B., Iboko, C. J. and Aladegbaye, T. A. (2022). Prevalence and Antibiogram of Bacterial Species in Litter of Selected Poultry Farms in Idi-Ayunre Community, Oyo State, Nigeria Advanced Research In Life Sciences, 6, 12- 18

[50] Podschun, R. and Ullmann, U. (2010). Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clinical Microbiology Reviews, 11(4), 589-603.

[51] Podschun, R., Pietsch, S., Holler, C. and Ullmann, U. (2001). Incidence of Klebsiella species in surface waters and their expression of virulence factors. Applied and Environmental Microbiology, 67 (7), 3325-3327.

[52] Rasmussen, M. M., Opintan, J. A., Frimodt-Moller, N. and Styrishave, B. (2015). Beta-lactamase producing Escherichia coli isolates in imported and locally produced chicken meat from Ghana. PLoS One, 10, e0139706.

[53] Ripabelli, G., Tamburro, M., Guerrizio, G., Fanelli, I., Flocco, R., Scutellà, M. and Sammarco, M. L. (2018). Tracking multidrug-resistant Klebsiella pneumoniae from an Italian hospital: Molecular epidemiology and surveillance by PFGE, RAPD and PCR-Based resistance genes prevalence. Current Microbiology, 75, 977–987

[54] Rosenblueth, M., Martinez, L., Silva, J. and Martinez-Romero, E. (2004). Klebsiella variicola, a novel species with clinical and plant-associated isolates.  Systematic and Applied Microbiology, 27(1), 27-35

[55] Seiffert, S. N., Carattoli, A., Schwendener, S., Collaud, A., Endimiani, A. and Perreten, V. (2017). Plasmids carrying blaCMY -2/4 in Escherichia coli from poultry, poultry meat, and humans belong to a novel IncK subgroup designated inck2. Frontiers Microbiology, 8, 407.

[56] Sohani S, and Sanjeeda I. (2012). Microbiological analysis of surface water in Indore. Research Journal of Recent Sciences, 1, 323-325.

[57] Sule H. and Kumurya A. S. (2016). “(e prevalence of Klebsiella species causing urinary tract infections in murtala Muhammad specialist hospital, Kano, Nigeria,” American Journal ofBiomedical and Life Sciences, 4 (2),. 11–15.

[58] Sung, H-W., Choi, K-S., Kwon, H-M. and Lee, Y-J. (2017). Patterns of antibiotic resistance in Escherichia coli isolated from fresh and recycled poultry litter. Korean Journal of Veterinamy Research, 57, 189-195.

[59] Susethira A. R. and Uma A. (2016). “Prevalence of klebsiella bacteriuria and antimicrobial susceptibility in a tertiary care hospital, Tiruchirapalli, India,” International Journal ofPharmaceutical Chemistry Research, 8 (6), 538–542.

[60] Tan, S. L., Lee, H. Y., Abu, B. F., Abdul, K. M. S, Rukayadi, Y. and Mahyudin, N. A. (2013). Microbiological quality on food handlers hands at primary schools in Hulu Langat District, Malaysia. International Food Research Journal, 20 (5), 2973- 2977.

[61] Thenmozhi, S., Rajeswari, P., Suresh, K.T., Saipriyanga, V. & Kalpana, M. (2014). Multi-drugresistant patterns of biofilm forming Aeromonashydrophila from urine samples. International Journal of Pharmaceutical Sciences and Research. 5(7): 2908-2918

[62] Van der Sluijs, M. T. W., Kuhn, E. M. and Makoschey, B. A. (2010). Single vaccination with an inactivated bovine respiratory syncytial virus vaccine primes the cellular immune response in calves with maternal antibody. BMC Veterinary Research, vol. 6, article 2.

[63] Wang, S. and Orsi, R.H. (2013). Foodborne Infections and Intoxications: Chapter 11. Listeria, Edition 4: Elsevier Inc. Chapters.

How to cite this paper

Adeluwoye-Ajayi, Olayemi Amos, Bello, Adedoyin Ayowole "Incidence and Antibiotic Resistance Pattern of Klebsiella Species Isolated from Poultry Feaces in Owo Metropolis" Iconic Research And Engineering Journals Volume 7 Issue 3 2023 Page 338-347
Adeluwoye-Ajayi, Olayemi Amos, Bello, Adedoyin Ayowole "Incidence and Antibiotic Resistance Pattern of Klebsiella Species Isolated from Poultry Feaces in Owo Metropolis" Iconic Research And Engineering Journals, vol. 7, no. 3, Sep. 2023
Adeluwoye-Ajayi, Olayemi Amos, Bello, Adedoyin Ayowole (2023). Incidence and Antibiotic Resistance Pattern of Klebsiella Species Isolated from Poultry Feaces in Owo Metropolis. Iconic Research And Engineering Journals, 7(3).
Adeluwoye-Ajayi, Olayemi Amos, Bello, Adedoyin Ayowole "Incidence and Antibiotic Resistance Pattern of Klebsiella Species Isolated from Poultry Feaces in Owo Metropolis" Iconic Research And Engineering Journals, vol. 7, no. 3, Sep. 2023.
@article{1705065,
      author = {Adeluwoye-Ajayi, Olayemi Amos, Bello, Adedoyin Ayowole},
      title = {Incidence and Antibiotic Resistance Pattern of Klebsiella Species Isolated from Poultry Feaces in Owo Metropolis},
      journal = {Iconic Research And Engineering Journals},
      year = {2023},
      volume = {7},
      number = {3},
      pages = {338-347},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1705065.pdf},
      abstract = {The wrong and excessive use of antibiotics in the poultry industry poses a threat to public health due to the release of antibiotic-resistant bacteria into the environment and its impact on consumers of poultry products. This research aimed to investigate the occurrence and patterns of antibiotic resistance found in Klebsiella species isolated from poultry faeces in Owo metropolis. Fecal samples were collected from healthy broilers in three privately-owned poultry farms in Owo, using sterile universal bottles and spatulas. Klebsiella species were isolated using MacConkey Agar, and their characteristics on Nutrient Agar were also observed. The isolated Klebsiella species exhibited different characteristics on different media types. All samples from the three farms contained Klebsiella species that were resistant to AUG and CAZ were susceptible to GEN, IMP, AZT, CIP, and NIT. A MARI score of 0.29 was obtained which indicates the presence of multidrug-resistant Klebsiella species in the bird excrement from poultry farms in Owo. Consequently, poultry breeders in Owo should exercise caution when employing antibiotics.},
      month = {September},
  }