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Current Trends in Nucleic Acids
Subject area: Biological & Medical Sciences · Area of research: Haematology/Transfusion Science
Abstract
Recent advancements in nucleic acid research have revolutionized our understanding of genetic regulation, diagnostics, and therapeutics. The emergence of next-generation RNA technologies, including RNA-based vaccines and RNA editing tools such as Adenosine Deaminase Acting on RNA (ADAR)-mediated systems, has expanded the utility of nucleic acids beyond traditional gene expression roles. In parallel, Clustered Regularly Interspaced Short Palindomic Repeats (CRISPR)-Cas systems have evolved into more precise, efficient, and programmable platforms for genome and epigenome editing, with innovations like CRISPR-Cas9 enabling nucleic acid detection and RNA manipulation. Moreover, nanopore sequencing and single-molecule real-time sequencing technologies are driving ultra-fast, high-resolution genomic analysis, empowering personalized medicine and microbiome profiling. Collectively, these trends mark a transformative era in nucleic acid research, fostering breakthroughs in precision medicine, synthetic biology, and disease diagnostics.
Keywords
Nucleic acid, Deoxyribonucleic acid, Ribonucleic acid, Therapeutics, Diagnostics, Gene editing, Vaccine, Vectors, Nanoparticles.
References
[1] Alameh MG, Tombacz I, Bettini E (2021). Vaccines by inducing robust T follicular helper cell and humoral responses. Journal of Immunity, 54: 2877 – 2892.
[2] Allen TM, Cullis PR (2013). Lipid nanoparticles enhance the efficacy of mRNA and protein subunit Liposomal drug delivery systems: from concept to clinical applications. Advanced Drug Delivery Review, 65(1): 36–48.
[3] Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., & Levy, J.M. (2019). Asymptomatic infections. Journal of Infectious Disease, 226: 920 – 927.
[4] Babaei, M., Eshghi, H., Abnous, K., Rahimizadeh, M., & Ramezani, M. (2017). Promising gene delivery system based on polyethylenimine- modified silica nanoparticles. Journal of Cancer Gene and Therapeutics, 24(4): 156 – 164.
[5] Bahl, K., Senn, J.J., & Yuzhakov, O. (2017). Preclinical and clinical demonstration of immunogenicity by mRNA vaccines against H10N8 and H7N9 influenza viruses. Journal of Molecular Therapy, 25: 1316–1327.
[6] Burdett, T., & Nuseibeh, S. (2023). Changing trends in the development of AAV-based gene therapies: a meta-analysis of past and present therapies. Gene Therapeutics, 30(4): 323–335.
[7] Chen, J., Chen, J., & Xu, Q. (2022). Current developments and challenges of mRNA vaccines. Annual Review of Biomedical Engineering, 24: 85–109.
[8] Cerritelli, S.M., & Crouch, R.J. (2009). Ribonucleases H: the enzymes in eukaryotes. Journal of Federation of European Biochemical Societies, 276: 1494 – 1505.
[9] Damase, T.R., Sukhovershin, R., Boada, C., Taraballi, F., Pettigrew, R.I., & Cooke, J.P. (2021). The Limitless Future of RNA Therapeutics. Journal of Frontier in Bioengineering and Biotechnology, 9: 628 – 637.
[10] Danhier, F. (2016). To exploit the tumour microenvironment: Since the EPR effect fails in the clinic, what is the future of nanomedicine? Journal Control Release, 244: 108–121.
[11] Davis, R. (2022). Nucleic acid testing applications in molecular diagnostics. Journal of Industrial Biotechnology, 6(4): 120.
[12] Dias, N., Dheur, S., Nielsen, P.E., Gryaznov, S., Van Aerschot, A., Herdewijn, P., Helene, C., & Saison-Behmoaras, T.E. (1999). Antisense PNA tridecamers targeted region of Haras mRNA arrest polypeptide chain elongation. Journal of Molecular Biology, 294: 403 – 416.
[13] Elbashir, S.M., Hrborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001). Duplexes of 21 – nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature Review, 411: 494 – 498.
[14] Fitzgerald, K., White, S., Borodovsky, A., Bettencourt, B.R., & Strahs, A. (2017). A highly durable RNAi therapeutic inhibitor of PCSK9. National England Journal of Medicine, 376(1): 41–51.
[15] Geisbert, T.W., Lee, A.C.H., & Robbins, M. (2010). Post exposure protection of non-human primates against a lethal Ebola virus challenge with RNA interference: a proof-of-concept study. Lancet, 375: 1896–1905.
[16] Hannay, E., & Sands, P. (2021). One year into the Covid-19 pandemic, testing is as vital as ever. Available at: https://www.telegraph.co.uk/global-health/science-and-disease/ one-year-covid-19-pandemic-testing-vital-ever/.
[17] Hasell, J., Mathieu, E., & Beltekian, D. (2020). A cross-country database of COVID-19 testing. Journal of Science Data, 7: 345.
[18] Hayashi, C.T.H., Cao, Y., & Clark, L.C. (2022). mRNA-LNP expressing PfCSP and Pfs25 vaccine candidates targeting infection and transmission of Plasmodium falciparum. Nature Partner Journal of Vaccines, 7: 155.
[19] Hoffman, S.L., Vekemans, J., Richie, T.L., Duffy, P.E. (2015). The march toward malaria vaccines. America Journal of Medicine, 49 (4): 319–333.
[20] Hu, B., Zhong, L., Weng, Y., Peng, L., Huang, Y., Zhao, Y., & Liang, X.J. (2020). Therapeutic siRNA: State of the art signal transduction. Journal of Targeted Therapy, 5: 101.
[21] Iuliano, A.D., Roguski, K.M., & Chang, H.H. (2018). Estimates of global seasonal influenza-associated respiratory mortality: a modelling study. Lancet, 391: 1285–1300.
[22] Jackson, N.A.C., Kester, K.E., Casimiro, D., Gurunathan, S., & DeRosa, F. (2020). The promise of mRNA vaccines: a biotech and industrial perspective. Journal of Nature Partner, 5: 11.
[23] Jagger, B.W., Dowd, K.A., & Chen, R.E. (2019). Protective efficacy of nucleic acid vaccines against transmission of Zika virus during pregnancy in mice. Journal of Infectious Disease, 220: 1577–1588.
[24] Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A., and Charpentier, E. Jirikowski G.F., Sanna, P.P., Maciejewski-Lenoir, D., & Bloom, F.E. (1992). Reversal of diabetes insipidus in Brattleboro rats: intrahypothalamic injection of vasopressin mRNA. Journal of Science, 255: 996–998.
[25] Kariko, K., Buckstein, M., Ni, H., & Weissman, D. (2005). Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA. Journal of Immunity, 23: 165–175.
[26] Kristen, A.V., Ajroud-Driss, S., Conceicao, I., Gorevic, P., kyriakides, T., & Obici, L.P. (2019). An RNAi therapeutic for te treatment of hereditary transthyretin-mediated Amyloidosis. Journal of Disease Management, 9: 5 – 23.
[27] Labatut, A.E., & Mattheolabakis, G. (2018). Non-viral based miR delivery and recent developments. European Journal of Pharmacology and Biopharmacology, 128: 82–90.
[28] Larsen, S.E., Baldwin, S.L., & Coler, R.N. (2023). Tuberculosis vaccines update: is an RNA-based vaccine feasible for tuberculosis? International Journal of Infectious Diseases, 130 (1): 47–51.
[29] Lee, K., Kim, M., Seo, Y., & Lee, H. (2018). Development of mRNA vaccines and their prophylactic and therapeutic applications. Journal of Nano Resource, 11: 5173 – 5192.
[30] Lee, S.H., Park, S.M., & Kim, B.N. (2019). Emerging ultrafast nucleic acid amplification technologies for next generation molecular diagnostics. Journal of Biosenseor and Bioelectron, 141: 111 – 148.
[31] Lewnard, J.A., & Cobey, S. (2018). Immune history and influenza vaccine effectiveness. Journal of Vaccines, 6: 28.
[32] Liang, X. H., Sun, H., Nichols, J.G., & Crooke, S.T. (2017). RNase H1 – Dependent Antisense Oligonucleotides are Robustly Active in Directing RNA Cleavage in Both the Cytoplasm and the Nucleus. Journal of Molecular Therapy, 25: 2075 – 2092.
[33] Lindgren, G., Ols, S., & Liang, F. (2017). Induction of robust B cell responses after influenza mRNA vaccination is accompanied by circulating hemagglutinin-specific ICOS+ PD-1+ CXCR3+ T follicular helper cells. Frontier of Immunology, 8: 1539.
[34] Lujan, H., Griffin, W.C., Taube, J.H., & Sayes, C.M. (2019). Synthesis and characterization of nanometer-sized liposomes for encapsulation and microRNA transfer to breast cancer cells. International Journal of Nanomedicine, 14: 5159–5173.
[35] Manikandan, C., Kaushik, A., & Sen, D. (2020). Viral vector: potential therapeutic for glioblastoma multiforme. Cancer Gene Therapeutics, 27(5): 270–279.
[36] Martinez, D.R., Schafer, A., & Leist, S.R. (2021). Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice. Journal of Science, 373: 991–998.
[37] Montefiori, D.C., Roederer, M., Morris, L., & Seaman, M.S. (2018). Neutralization tiers of HIV-1. Curriculum Opined on HIV AIDS, 13: 128–136.
[38] Mu, Z., Haynes, B.F., & Cain, D.W. (2021). HIV mRNA vaccines-progress and future paths. Journal of Vaccines, 9: 134.
[39] Mu, Z., Wiehe, K., & Saunders, K.O. (2022). mRNA-encoded HIV-1 Env trimer ferritin nanoparticles induce monoclonal antibodies that neutralize heterologous HIV-1 isolates in mice. Journal of Cell Replication, 38: 110514.
[40] Naeem, S., Zhang, J., Zhang, Y., & Wang, Y. (2025). Nucleic Acid therapeutics: past, present, and future. Journal of Molecular Therapy, 36: 1 -25.
[41] Nambiar, T.S., Baudrier, L., Billon, P., & Ciccia, A. (2022). CRISPR-based genome editing through the lens of DNA repair. Journal of Molecular Cell, 82: 348 – 388.
[42] Ondoa, P., Kebede, Y., & Massinga, M. (2020). COVID-19 testing in Africa: lessons learnt. Lancet Microbe, 1:103–104.
[43] Pacesa, M., Pelea, O., & Jinek, M. (2024). Past, present, and future of CRISPR genome editing technologies. Journal of Cell, 187: 1076 – 1100.
[44] Pardi, N., Hogan, M.J., & Pelc, R.S. (2017). Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination. Journal of Nature, 543: 248–251.
[45] Pattnaik, A., Sahoo, B.R., & Pattnaik, A.K. (2020). Current status of Zika virus vaccines: successes and challenges. Journal of Vaccines, 8: 266.
[46] Raguram, A., Banskota, S., & Liu, D.R. (2022). Therapeutic in vivo delivery of gene editing agents. Journal of Cell, 185: 2806 – 2827.
[47] Raj, D.K., Das Mohapatra, A., & Jnawali, A. (2020). Anti-PfGARP activates programmed cell death of parasites and reduces severe malaria. Journal of Nature, 582: 104–108.
[48] Richner, J.M, Himansu, S., & Dowd, K.A. (2017). Modified mRNA vaccines protect against Zika virus infection. Journal of Cell, 168: 1114–1125.
[49] Rinaldi, C., & Wood, M.J.A. (2018). Antisense oligonucleotides: the next frontier for treatment of neurological disorders. Nature Review, 14: 9 – 21.
[50] Rurik, J.G., Tombacz, I., & Yadegari, A. (2022). CAR T cells produced in vivo to treat cardiac injury. Journal of Science, 375: 91–99.
[51] Sato, Y., Sakurai, Y., Kajimoto, K., Nakamura, T., Yamada, Y., Akita H (2017). Innovative technologies in nanomedicines: from passive targeting to active targeting/from controlled pharmacokinetics to controlled intracellular pharmacokinetics. Macromolecule Bioscience, 17(1): 1600179.
[52] Saunders KO, Pardi N, Parks R (2021). Lipid nanoparticle encapsulated nucleoside-modified mRNA vaccines elicit polyfunctional HIV-1 antibodies comparable to proteins in zika virus. Nature Review, 5: 105 – 112.
[53] Schnee, M., Vogel, A.B., & Voss, D. (2016). An mRNA vaccine encoding rabies virus glycoprotein induces protection against lethal infection in mice and correlates of protection in adult and newborn pigs. Public Library of Science, 10: 4746.
[54] Schrager, L.K., Vekemens, J., Drager, N., Lewinsohn, D.M., & Olesen, O.F. (2020). The status of tuberculosis vaccine development. Lancet Infectious Diseases, 20: 28–37.
[55] Sung YK, Kim SW (2019). Recent advances in the development of gene delivery systems. Biomaterial Resource, 23: 8.
[56] Waghela, I.N., Mallory, K.L., & Taylor J.A. (2022). Exploring in vitro expression and immune potency in mice using mRNA encoding the Plasmodium falciparum malaria antigen, CelTOS. Frontier of Immunology, 13: 1026052.
[57] Wang, Q., Liang, Q., Dou, J., Zhou, H., Zeng, C., & Pan, H. (2023). Breaking through the basement membrane barrier to improve nanotherapeutic delivery to tumours. Nature Nanotechnology, 19: 95.
[58] Wang, Y., Bruggeman, K.F, Franks, S., Gautam, V., Hodgetts, S.I., Harvey, A.R. (2022). Is viral vector gene delivery more effective using biomaterials? 10(1): 1238.
[59] Wang, P., He, D., Li, Y., Wang, W., & Luo, X. (2019). Eliminating mcr-1-harbouring plasmids in clinical isolates using the CRISPR/cas 9 system. Journal of Antimicrobiology and Chemotherapy, 74: 2559 – 2565.
[60] Whiley, D.M., Tapsall, J.W., & Sloots, T.P. (2006). Nucleic acid amplification testing for Neisseria gonorrhoeae: an ongoing challenge. Journal of Molecular Diagnostics, 8(1): 1 – 15.
[61] Wittrup, A., & Lieberman, J. (2015). Knocking down disease: a progress report on siRNA therapeutics. Journal of Nature Review and Genetics, 16: 543 – 55
[62] Wu Y, Tang Y, Xie S, Zheng X, Zhang S, Mao J (2020). Chimeric peptide supramolecular nanoparticles for plectin-1targeted miRNA-9 delivery in pancreatic cancer. 10(3): 1151–1165.
[63] Xiong, Q., Lee, G.Y., Ding, J., Li, W., Shi J (2018). Biomedical applications of mRNA nanomedicine. Nanomedicine Resource, 11(10): 5281–5309.
[64] Xue T, Stavropoulos E, Yang M (2004). RNA encoding the MPT83 antigen induces protective immune responses against Mycobacterium tuberculosis infection. Journal of Infectious Immunology, 72: 6324–6329.
[65] Young LS, Searle PF, Onion D, Mautner V (2006). Viral gene therapy strategies: from basic science to clinical application. Journal of Pathology, 208(2): 299–318.
[66] Zhang C, Maruggi G, Shan H, Li J ( 2019). Advances in mRNA vaccines for infectious diseases. Frontier of Immunology, 10: 594.
[67] Zhang L, Liao Y, Tang L (2019). MicroRNA-34 family: a potential tumour suppressor and therapeutic candidate in cancer. Journal of Experimental Clinical Cancer Resource, 38(1): 1170.
How to cite this paper
@article{1710336,
author = {Okereke, Chibueze Aphogu, Prof. Ufelle Silas},
title = {Current Trends in Nucleic Acids},
journal = {Iconic Research And Engineering Journals},
year = {2025},
volume = {9},
number = {2},
pages = {1052-1063},
issn = {2456-8880},
url = {https://www.irejournals.com/formatedpaper/1710336.pdf},
abstract = {Recent advancements in nucleic acid research have revolutionized our understanding of genetic regulation, diagnostics, and therapeutics. The emergence of next-generation RNA technologies, including RNA-based vaccines and RNA editing tools such as Adenosine Deaminase Acting on RNA (ADAR)-mediated systems, has expanded the utility of nucleic acids beyond traditional gene expression roles. In parallel, Clustered Regularly Interspaced Short Palindomic Repeats (CRISPR)-Cas systems have evolved into more precise, efficient, and programmable platforms for genome and epigenome editing, with innovations like CRISPR-Cas9 enabling nucleic acid detection and RNA manipulation. Moreover, nanopore sequencing and single-molecule real-time sequencing technologies are driving ultra-fast, high-resolution genomic analysis, empowering personalized medicine and microbiome profiling. Collectively, these trends mark a transformative era in nucleic acid research, fostering breakthroughs in precision medicine, synthetic biology, and disease diagnostics. },
keywords = {Nucleic acid, Deoxyribonucleic acid, Ribonucleic acid, Therapeutics, Diagnostics, Gene editing, Vaccine, Vectors, Nanoparticles. },
month = {August},
}