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The Effect of pH on Steady-State Fermentation of Soya Bean

Rufai Nasir Abubakar Mannir Rawayau Ubiri Benjamin Oghenekparobo Olurinde Victoria Oluwapelumi Lateefat Ololade Mustapha Saleem Adetunji Adeniyi Hamza Magaji

Subject area: Science,Engineering and Technology  ·  Area of research: Food Technology

Abstract

Fermentation is also crucial in nutritional and sensory improvements of soybeans thus rendering them to be more food-friendly. To enable the steady fermentation of soybean pods, the research analyses the effects of pH and temperature on three variables, including protein content, moisture level, and growth of microbes. It is carried out with an inoculation of Bacillus subtilis and Lactobacillus plantarum mixed culture and underlying the methodological approach response-surface methodology is used to determine the most favourable operating conditions. The results showed that acidic pH especially at pH of 3 was found to have the greatest protein enrichment and also promoted maximum bacterial activity at moderate temperatures between 25 and 30 o C. The protein content fell at higher pH and high temperatures, the level of moisture also fell with increase in temperature. These results demonstrate that the quality of the products and the performance of the microorganisms depend on the control of the fermentation environment of these microorganisms. This work contributes to a better comprehension of steady processes related to the soybean fermentation, clarifying the influence of pH and temperature on fermentation and improving the practise in the industries. Finally, the investigation demonstrates that nutritional benefits can be enhanced through controlled fermentation in order to raise the chances of sustainable use of soybean.

Keywords

Fermentation, pH, Protein content, Soybean, Temperature

References

[1] Chen, K. I., Erh, M. H., Su, N. W., Liu, W. H., Chou, C. C., & Cheng, K. C. (2012). Soyfoods and soybean products: from traditional use to modern applications. Applied microbiology and biotechnology, 96(1), 9-22.

[2] Rizzo, G., & Baroni, L. (2018). Soy, soy foods and their role in vegetarian diets. Nutrients, 10(1), 43.

[3] Reinwald, S., Akabas, S. R., & Weaver, C. M. (2010). Whole versus the piecemeal approach to evaluating soy. The Journal of nutrition, 140(12), 2335S-2343S.

[4] Tamang, J. P., Sarkar, P. K., & Hesseltine, C. W. (1988). Traditional fermented foods and beverages of Darjeeling and Sikkim–a review. Journal of the Science of Food and Agriculture, 44(4), 375-385.

[5] Mukherjee, R., Chakraborty, R., & Dutta, A. (2015). Role of fermentation in improving nutritional quality of soybean meal—a review. Asian-Australasian journal of animal sciences, 29(11), 1523.

[6] Hu, C., Wong, W. T., Wu, R., & Lai, W. F. (2020). Biochemistry and use of soybean isoflavones in functional food development. Critical Reviews in Food Science and Nutrition, 60(12), 2098-2112.

[7] Lee, I. B., Choi, K. J., Yu, K. K., & Chang, K. W. (1992). Tocopherols and fatty acids in plant seeds from Korea. Applied Biological Chemistry, 35(1), 1-5.

[8] Rollán, G. C., Gerez, C. L., & LeBlanc, J. G. (2019). Lactic fermentation as a strategy to improve the nutritional and functional values of pseudocereals. Frontiers in Nutrition, 6, 98.

[9] Tangyu, M., Muller, J., Bolten, C. J., & Wittmann, C. (2019). Fermentation of plant-based milk alternatives for improved flavour and nutritional value. Applied microbiology and biotechnology, 103(23), 9263-9275.

[10] Messina, M. (2016). Soy and health update: evaluation of the clinical and epidemiologic literature. Nutrients, 8(12), 754.

[11] Moses, T., Papadopoulou, K. K., & Osbourn, A. (2014). Metabolic and functional diversity of saponins, biosynthetic intermediates and semi-synthetic derivatives. Critical reviews in biochemistry and molecular biology, 49(6), 439-462.

[12] Podolak, I., Galanty, A., & Sobolewska, D. (2010). Saponins as cytotoxic agents: a review. Phytochemistry Reviews, 9(3), 425-474.

[13] Ganesan, K., & Xu, B. (2017). A critical review on polyphenols and health benefits of black soybeans. Nutrients, 9(5), 455.

[14] Neacsu, M., Raikos, V., Benavides-Paz, Y., Duncan, S. H., Duncan, G. J., Christie, J. S., ... & Russell, W. R. (2020). Sapogenol is a major microbial metabolite in human plasma associated with high protein soy-based diets: the relevance for functional food formulations. Foods, 9(4), 422.

[15] Myers, R. H., Montgomery, D. C., & Anderson-Cook, C. M. (2016). Response surface methodology: process and product optimization using designed experiments. John Wiley & Sons.

[16] Shabbir, U., Tyagi, A., Ham, H. J., Elahi, F., & Oh, D. H. (2022). Effect of fermentation on the bioactive compounds of the black soybean and their anti-Alzheimer’s activity. Frontiers in nutrition, 9, 880361.

[17] Nielsen, S. S. (2017). Moisture content determination. In Food analysis laboratory manual (pp. 105-115). Cham: Springer International Publishing.

[18] Mariam, S. H., Zegeye, N., Tariku, T., Andargie, E., Endalafer, N., & Aseffa, A. (2014). Potential of cell-free supernatants from cultures of selected lactic acid bacteria and yeast obtained from local fermented foods as inhibitors of Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus. BMC research notes, 7(1), 606.

[19] Stone, H., Bleibaum, R. N., & Thomas, H. A. (2020). Sensory evaluation practices. Academic press.

[20] Montgomery, D. C. (2017). Design and analysis of experiments. John wiley & sons.

[21] Behera, S. S., Ray, R. C., & Zdolec, N. (2018). Lactobacillus plantarum with functional properties: an approach to increase safety and shelf‐life of fermented foods. BioMed research international, 2018(1), 9361614.

How to cite this paper

Rufai Nasir, Abubakar Mannir Rawayau, Ubiri Benjamin Oghenekparobo, Olurinde Victoria Oluwapelumi; Lateefat Ololade Mustapha, Saleem Adetunji Adeniyi; Hamza Magaji "The Effect of pH on Steady-State Fermentation of Soya Bean" Iconic Research And Engineering Journals Volume 9 Issue 3 2025 Page 1896-1900
Rufai Nasir, Abubakar Mannir Rawayau, Ubiri Benjamin Oghenekparobo, Olurinde Victoria Oluwapelumi; Lateefat Ololade Mustapha, Saleem Adetunji Adeniyi; Hamza Magaji "The Effect of pH on Steady-State Fermentation of Soya Bean" Iconic Research And Engineering Journals, vol. 9, no. 3, Sep. 2025
Rufai Nasir, Abubakar Mannir Rawayau, Ubiri Benjamin Oghenekparobo, Olurinde Victoria Oluwapelumi; Lateefat Ololade Mustapha, Saleem Adetunji Adeniyi; Hamza Magaji (2025). The Effect of pH on Steady-State Fermentation of Soya Bean. Iconic Research And Engineering Journals, 9(3).
Rufai Nasir, Abubakar Mannir Rawayau, Ubiri Benjamin Oghenekparobo, Olurinde Victoria Oluwapelumi; Lateefat Ololade Mustapha, Saleem Adetunji Adeniyi; Hamza Magaji "The Effect of pH on Steady-State Fermentation of Soya Bean" Iconic Research And Engineering Journals, vol. 9, no. 3, Sep. 2025.
@article{1710381,
      author = {Rufai Nasir, Abubakar Mannir Rawayau, Ubiri Benjamin Oghenekparobo, Olurinde Victoria Oluwapelumi; Lateefat Ololade Mustapha, Saleem Adetunji Adeniyi; Hamza Magaji},
      title = {The Effect of pH on Steady-State Fermentation of Soya Bean},
      journal = {Iconic Research And Engineering Journals},
      year = {2025},
      volume = {9},
      number = {3},
      pages = {1896-1900},
      issn = {2456-8880},
      url = {https://www.irejournals.com/formatedpaper/1710381.pdf},
      abstract = {Fermentation is also crucial in nutritional and sensory improvements of soybeans thus rendering them to be more food-friendly. To enable the steady fermentation of soybean pods, the research analyses the effects of pH and temperature on three variables, including protein content, moisture level, and growth of microbes. It is carried out with an inoculation of Bacillus subtilis and Lactobacillus plantarum mixed culture and underlying the methodological approach response-surface methodology is used to determine the most favourable operating conditions. The results showed that acidic pH especially at pH of 3 was found to have the greatest protein enrichment and also promoted maximum bacterial activity at moderate temperatures between 25 and 30 o C. The protein content fell at higher pH and high temperatures, the level of moisture also fell with increase in temperature. These results demonstrate that the quality of the products and the performance of the microorganisms depend on the control of the fermentation environment of these microorganisms. This work contributes to a better comprehension of steady processes related to the soybean fermentation, clarifying the influence of pH and temperature on fermentation and improving the practise in the industries. Finally, the investigation demonstrates that nutritional benefits can be enhanced through controlled fermentation in order to raise the chances of sustainable use of soybean.},
      keywords = {Fermentation, pH, Protein content, Soybean, Temperature},
      month = {September},
  }